Construct: ORF TRCN0000491519

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020566.2_s317c1
DNA Barcode:
CTTACGAAATCTTTTTAATGGCCG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MAP3K12 (7786)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491519
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7786 MAP3K12 mitogen-activated protein k... NM_006301.4 77.8% 77.7% 2004_2010delAGTAGGG;2014_2577delinsC
2 human 7786 MAP3K12 mitogen-activated protein k... XM_017019956.2 77.8% 77.7% 2004_2010delAGTAGGG;2014_2577delinsC
3 human 7786 MAP3K12 mitogen-activated protein k... NM_001193511.2 74.9% 74.7% 138_236del;2103_2109delAGTAGGG;2113_2676delinsC
4 human 7786 MAP3K12 mitogen-activated protein k... XM_005269138.4 74.9% 74.7% 138_236del;2103_2109delAGTAGGG;2113_2676delinsC
5 human 7786 MAP3K12 mitogen-activated protein k... XM_006719588.4 74.9% 74.7% 138_236del;2103_2109delAGTAGGG;2113_2676delinsC
6 human 7786 MAP3K12 mitogen-activated protein k... XM_011538725.3 74.9% 74.7% 138_236del;2103_2109delAGTAGGG;2113_2676delinsC
7 human 7786 MAP3K12 mitogen-activated protein k... XM_005269140.2 59.7% 59.6% 138_236del;1359_1359delGins748
8 mouse 26404 Map3k12 mitogen-activated protein k... NM_001163643.1 68.5% 73.7% (many diffs)
9 mouse 26404 Map3k12 mitogen-activated protein k... NM_009582.4 68.5% 73.7% (many diffs)
10 mouse 26404 Map3k12 mitogen-activated protein k... XM_017316624.1 68.5% 73.7% (many diffs)
11 mouse 26404 Map3k12 mitogen-activated protein k... XM_017316625.1 68.5% 73.7% (many diffs)
12 mouse 26404 Map3k12 mitogen-activated protein k... XR_001781538.1 46.8% (many diffs)
13 mouse 26404 Map3k12 mitogen-activated protein k... XR_001781537.1 42.6% (many diffs)
14 mouse 26404 Map3k12 mitogen-activated protein k... XM_017316626.1 32.4% 28.3% (many diffs)
15 mouse 26404 Map3k12 mitogen-activated protein k... XM_006521013.2 32.3% 28.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
84
ORF end:
2091
ORF length:
2007
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggctccgc ggcccccttc accatggctt gcctccatga gacccgaaca ccctctcctt
121cctttggggg ctttgtgtct accctaagtg aggcatccat gcgcaagctg gacccagaca
181cttctgactg cactcccgag aaggacctga cgcctaccca tgtcctgcag ctacatgagc
241aggatgcagg gggcccaggg ggagcagctg ggtcacctga gagtcgggca tccagagttc
301gagctgacga ggtgcgactg cagtgccaga gtggcagtgg cttccttgag ggcctctttg
361gctgcctgcg ccctgtctgg accatgattg gcaaagccta ctccactgag cacaagcagc
421agcaggaaga cctttgggag gtcccctttg aggaaatcct ggacctgcag tgggtgggct
481caggggccca gggtgctgtc ttcctggggc gcttccacgg ggaggaggtg gctgtgaaga
541aggtgcgaga cctcaaagaa accgacatca agcacttgcg aaagctgaag caccccaaca
601tcatcacttt caagggtgtg tgcacccagg ctccctgcta ctgcatcctc atggagttct
661gcgcccaggg ccagctgtat gaggtactgc gggctggccg ccctgtcacc ccctccttac
721tggttgactg gtccatgggc atcgctggtg gcatgaacta cctgcacctg cacaagatta
781tccacaggga tctcaagtca cccaacatgc taatcaccta cgacgatgtg gtgaagatct
841cagattttgg cacttccaag gagctgagtg acaagagcac caagatgtcc tttgcaggga
901cagtagcctg gatggcccct gaggtgatcc gcaatgaacc tgtgtctgag aaggtcgaca
961tctggtcctt tggcgtggtg ctatgggaac tgctgactgg tgagatcccc tacaaagacg
1021tagattcctc agccattatc tggggtgtgg gaagcaacag tctccatctg cccgtgccct
1081ccagttgccc agatggtttc aagatcctgc ttcgccagtg ctggaatagc aaaccacgaa
1141atcgcccatc attccgacag atcctgctgc atctggacat tgcctcagct gatgtactct
1201ccacacccca ggagacttac tttaagtccc aggcagagtg gcgggaagaa gtaaaactgc
1261actttgaaaa gattaagtca gaagggacct gtctgcaccg cctagaagag gaactggtga
1321tgaggaggag ggaggagctc agacacgccc tggacatcag ggagcactat gaaaggaagc
1381tggagagagc caacaacctg tatatggaac ttaatgccct catgttgcag ctggaactca
1441aggagaggga gctgctcagg cgagagcaag ctttagagcg gaggtgccca ggcctgctga
1501agccacaccc ttcccggggc ctcctgcatg gaaacacaat ggagaagctt atcaagaaga
1561ggaatgtgcc acagaagctg tcaccccata gcaaaaggcc agatatcctc aagacggagt
1621ctttgctccc taaactagat gcagccctga gtggggtggg gcttcctggg tgtcctaagg
1681gccccccctc accaggacgg agtcgccgtg gcaagacccg tcaccgcaag gccagcgcca
1741aggggagctg tggggacctg cctgggcttc gtacagctgt gccaccccat gaacctggag
1801gaccaggaag cccagggggc ctaggagggg gaccctcagc ctgggaggcc tgccctcccg
1861ccctccgtgg gcttcatcat gacctcctgc TCCGCAAAAT GTCTTCATCG TCCCCAGACC
1921TGCTGTCAGC AGCACTAGGG TCCCGGGGCC GGGGGGCCAC AGGCGGAGCT GGGGATCCTG
1981GCTCACCACC TCCGGCCCGG GGTGACACCC CACCAAGTGA GGGCTCAGCC CCTGGCTCCA
2041CCAGCCCAGA TTCACCTGGG GGAGCCAAAG GGGAACCACC TCCTCCCCTC TGAGGTTTCT
2101CAGGTGGCAA TGTAACTCAT TTTTTTGTCC CACCATTTAT CTTCTCTGCC CAAGCCCTGT
2161CTTAAGGCCC AGGGGGAGGT TAGGAGACTG ATCTAGAAAG GGTGGGCGCG CCGACCCAGC
2221TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA
2281TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT
2341ATCTTGTGGA AAGGACGACT TACGAAATCT TTTTAATGGC CGACGCGTTA AGTCgacaat
2401caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)

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