Construct: ORF TRCN0000491519
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020566.2_s317c1
- DNA Barcode:
- CTTACGAAATCTTTTTAATGGCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K12 (7786)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491519
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7786 | MAP3K12 | mitogen-activated protein k... | NM_006301.4 | 77.8% | 77.7% | 2004_2010delAGTAGGG;2014_2577delinsC |
| 2 | human | 7786 | MAP3K12 | mitogen-activated protein k... | XM_017019956.2 | 77.8% | 77.7% | 2004_2010delAGTAGGG;2014_2577delinsC |
| 3 | human | 7786 | MAP3K12 | mitogen-activated protein k... | NM_001193511.2 | 74.9% | 74.7% | 138_236del;2103_2109delAGTAGGG;2113_2676delinsC |
| 4 | human | 7786 | MAP3K12 | mitogen-activated protein k... | XM_005269138.4 | 74.9% | 74.7% | 138_236del;2103_2109delAGTAGGG;2113_2676delinsC |
| 5 | human | 7786 | MAP3K12 | mitogen-activated protein k... | XM_006719588.4 | 74.9% | 74.7% | 138_236del;2103_2109delAGTAGGG;2113_2676delinsC |
| 6 | human | 7786 | MAP3K12 | mitogen-activated protein k... | XM_011538725.3 | 74.9% | 74.7% | 138_236del;2103_2109delAGTAGGG;2113_2676delinsC |
| 7 | human | 7786 | MAP3K12 | mitogen-activated protein k... | XM_005269140.2 | 59.7% | 59.6% | 138_236del;1359_1359delGins748 |
| 8 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | NM_001163643.1 | 68.5% | 73.7% | (many diffs) |
| 9 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | NM_009582.4 | 68.5% | 73.7% | (many diffs) |
| 10 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | XM_017316624.1 | 68.5% | 73.7% | (many diffs) |
| 11 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | XM_017316625.1 | 68.5% | 73.7% | (many diffs) |
| 12 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | XR_001781538.1 | 46.8% | (many diffs) | |
| 13 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | XR_001781537.1 | 42.6% | (many diffs) | |
| 14 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | XM_017316626.1 | 32.4% | 28.3% | (many diffs) |
| 15 | mouse | 26404 | Map3k12 | mitogen-activated protein k... | XM_006521013.2 | 32.3% | 28.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 2091
- ORF length:
- 2007
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatggctt gcctccatga gacccgaaca ccctctcctt 121 cctttggggg ctttgtgtct accctaagtg aggcatccat gcgcaagctg gacccagaca 181 cttctgactg cactcccgag aaggacctga cgcctaccca tgtcctgcag ctacatgagc 241 aggatgcagg gggcccaggg ggagcagctg ggtcacctga gagtcgggca tccagagttc 301 gagctgacga ggtgcgactg cagtgccaga gtggcagtgg cttccttgag ggcctctttg 361 gctgcctgcg ccctgtctgg accatgattg gcaaagccta ctccactgag cacaagcagc 421 agcaggaaga cctttgggag gtcccctttg aggaaatcct ggacctgcag tgggtgggct 481 caggggccca gggtgctgtc ttcctggggc gcttccacgg ggaggaggtg gctgtgaaga 541 aggtgcgaga cctcaaagaa accgacatca agcacttgcg aaagctgaag caccccaaca 601 tcatcacttt caagggtgtg tgcacccagg ctccctgcta ctgcatcctc atggagttct 661 gcgcccaggg ccagctgtat gaggtactgc gggctggccg ccctgtcacc ccctccttac 721 tggttgactg gtccatgggc atcgctggtg gcatgaacta cctgcacctg cacaagatta 781 tccacaggga tctcaagtca cccaacatgc taatcaccta cgacgatgtg gtgaagatct 841 cagattttgg cacttccaag gagctgagtg acaagagcac caagatgtcc tttgcaggga 901 cagtagcctg gatggcccct gaggtgatcc gcaatgaacc tgtgtctgag aaggtcgaca 961 tctggtcctt tggcgtggtg ctatgggaac tgctgactgg tgagatcccc tacaaagacg 1021 tagattcctc agccattatc tggggtgtgg gaagcaacag tctccatctg cccgtgccct 1081 ccagttgccc agatggtttc aagatcctgc ttcgccagtg ctggaatagc aaaccacgaa 1141 atcgcccatc attccgacag atcctgctgc atctggacat tgcctcagct gatgtactct 1201 ccacacccca ggagacttac tttaagtccc aggcagagtg gcgggaagaa gtaaaactgc 1261 actttgaaaa gattaagtca gaagggacct gtctgcaccg cctagaagag gaactggtga 1321 tgaggaggag ggaggagctc agacacgccc tggacatcag ggagcactat gaaaggaagc 1381 tggagagagc caacaacctg tatatggaac ttaatgccct catgttgcag ctggaactca 1441 aggagaggga gctgctcagg cgagagcaag ctttagagcg gaggtgccca ggcctgctga 1501 agccacaccc ttcccggggc ctcctgcatg gaaacacaat ggagaagctt atcaagaaga 1561 ggaatgtgcc acagaagctg tcaccccata gcaaaaggcc agatatcctc aagacggagt 1621 ctttgctccc taaactagat gcagccctga gtggggtggg gcttcctggg tgtcctaagg 1681 gccccccctc accaggacgg agtcgccgtg gcaagacccg tcaccgcaag gccagcgcca 1741 aggggagctg tggggacctg cctgggcttc gtacagctgt gccaccccat gaacctggag 1801 gaccaggaag cccagggggc ctaggagggg gaccctcagc ctgggaggcc tgccctcccg 1861 ccctccgtgg gcttcatcat gacctcctgc TCCGCAAAAT GTCTTCATCG TCCCCAGACC 1921 TGCTGTCAGC AGCACTAGGG TCCCGGGGCC GGGGGGCCAC AGGCGGAGCT GGGGATCCTG 1981 GCTCACCACC TCCGGCCCGG GGTGACACCC CACCAAGTGA GGGCTCAGCC CCTGGCTCCA 2041 CCAGCCCAGA TTCACCTGGG GGAGCCAAAG GGGAACCACC TCCTCCCCTC TGAGGTTTCT 2101 CAGGTGGCAA TGTAACTCAT TTTTTTGTCC CACCATTTAT CTTCTCTGCC CAAGCCCTGT 2161 CTTAAGGCCC AGGGGGAGGT TAGGAGACTG ATCTAGAAAG GGTGGGCGCG CCGACCCAGC 2221 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 2281 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 2341 ATCTTGTGGA AAGGACGACT TACGAAATCT TTTTAATGGC CGACGCGTTA AGTCgacaat 2401 caacctctgg attacaaaat ttgtgaaaga tt