Construct: ORF TRCN0000491548
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019850.2_s317c1
- DNA Barcode:
- AAGAATCCAGGAGATGAAACCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP4K3 (8491)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491548
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8491 | MAP4K3 | mitogen-activated protein k... | NM_003618.4 | 99.9% | 99.8% | 189T>C;2682_2683insG |
| 2 | human | 8491 | MAP4K3 | mitogen-activated protein k... | NM_001270425.2 | 97.5% | 97.5% | 189T>C;1056_1057ins63;2619_2620insG |
| 3 | human | 8491 | MAP4K3 | mitogen-activated protein k... | XR_939730.2 | 60.2% | (many diffs) | |
| 4 | human | 8491 | MAP4K3 | mitogen-activated protein k... | XM_024453183.1 | 49.9% | 49.9% | 0_1ins1341;1341_1342insG |
| 5 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | NM_001290345.1 | 88.9% | 95.9% | (many diffs) |
| 6 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524192.2 | 87.8% | 94.8% | (many diffs) |
| 7 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524196.2 | 87.2% | 94% | (many diffs) |
| 8 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524197.2 | 86.6% | 93.7% | (many diffs) |
| 9 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524194.2 | 86.1% | 92.9% | (many diffs) |
| 10 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524195.2 | 85.5% | 92.6% | (many diffs) |
| 11 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524198.2 | 84.9% | 91.8% | (many diffs) |
| 12 | mouse | 225028 | Map4k3 | mitogen-activated protein k... | XM_006524199.3 | 78.4% | 84.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2754
- ORF length:
- 2685
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaaccccggc ttcgatttgt cccgccggaa cccgcaggag gacttcgagc 121 tgattcagcg catcggcagc ggcacctacg gcgacgtcta caaggcacgg aatgttaaca 181 ctggtgaatt agcagcaatt aaagtaataa aattggaacc aggagaagac tttgcagttg 241 tgcagcaaga aattatcatg atgaaagact gtaaacaccc aaatattgtt gcttattttg 301 gaagctatct caggcgagat aagctttgga tttgcatgga gttttgtgga ggtggttctt 361 tacaggatat ttatcacgta actggacctc tgtcagaact gcaaattgca tatgttagca 421 gagaaacact gcagggatta tattatcttc acagtaaagg aaaaatgcac agagatataa 481 agggagctaa cattctatta acggataatg gtcatgtgaa attggctgat tttggagtat 541 ctgcacagat aacagctaca attgccaaac ggaagtcttt cattggcaca ccatattgga 601 tggctccaga agttgcagct gttgagagga aggggggtta caatcaactc tgtgatctct 661 gggcagtggg aatcactgcc atagaacttg cagagcttca gcctcctatg tttgacttac 721 acccaatgag agcattattt ctaatgacaa aaagcaattt tcagcctcct aaactaaagg 781 ataaaatgaa atggtcaaat agttttcatc actttgtgaa aatggcactt accaaaaatc 841 cgaaaaaaag acctactgct gaaaaattat tacagcatcc ttttgtaaca caacatttga 901 cacggtcttt ggcaatcgag ctgttggata aagtaaataa tccagatcat tccacttacc 961 atgatttcga tgatgatgat cctgagcctc ttgttgctgt accacataga attcactcaa 1021 caagtagaaa cgtgagagaa gaaaaaacac gctcagagat aacctttggc caagtgaaat 1081 ttgatccacc cttaagaaag gagacagaac cacatcatga acttcccgac agtgatggtt 1141 ttttggacag ttcagaagaa atatactaca ctgcaagatc taatctggat ctgcaactgg 1201 aatatggaca aggacaccaa ggtggttact ttttaggtgc aaacaagagt cttctcaagt 1261 ctgttgaaga agaattgcat cagcgaggac acgtcgcaca tttagaagat gatgaaggag 1321 atgatgatga atctaaacac tcaactctga aagcaaaaat tccacctcct ttgccaccaa 1381 agcctaagtc tatcttcata ccacaggaaa tgcattctac tgaggatgaa aatcaaggaa 1441 caatcaagag atgtcccatg tcagggagcc cagcaaagcc atcccaagtt ccacctagac 1501 caccacctcc cagattaccc ccacacaaac ctgttgcctt aggaaatgga atgagctcct 1561 tccagttaaa tggtgaacga gatggctcat tatgtcaaca acagaatgaa catagaggca 1621 caaacctttc aagaaaagaa aagaaagatg taccaaagcc tattagtaat ggtcttcctc 1681 caacacctaa agtgcatatg ggtgcatgtt tttcaaaagt ttttaatggg tgtcccttga 1741 aaattcactg tgcatcatca tggataaacc cagatacaag agatcagtac ttgatatttg 1801 gtgccgaaga agggatttat accctcaatc ttaatgaact tcatgaaaca tcaatggaac 1861 agctattccc tcgaaggtgt acatggttgt atgtaatgaa caattgcttg ctatcaatat 1921 ctggtaaagc ttctcagctt tattcccata atttaccagg gctttttgat tatgcaagac 1981 aaatgcaaaa gttacctgtt gctattccag cacacaaact ccctgacaga atactgccaa 2041 ggaaattttc tgtatcagca aaaatccctg aaaccaaatg gtgccagaag tgttgtgttg 2101 taagaaatcc ttacacgggc cataaatacc tatgtggagc acttcagact agcattgttc 2161 tattagaatg ggttgaacca atgcagaaat ttatgttaat taagcacata gattttccta 2221 taccatgtcc acttagaatg tttgaaatgc tggtagttcc tgaacaggag taccctttag 2281 tttgtgttgg tgtcagtaga ggtagagact tcaaccaagt ggttcgattt gagacggtca 2341 atccaaattc tacctcttca tggtttacag aaTCAGATAC CCCACAGACA AATGTTACTC 2401 ATGTAACCCA ACTGGAGAGA GATACCATCC TTGTATGCTT GGACTGTTGT ATAAAAATAG 2461 TAAATCTCCA AGGAAGATTA AAATCTAGCA GGAAATTGTC ATCAGAACTC ACCTTTGATT 2521 TCCAGATTGA ATCAATAGTG TGCCTACAAG ACAGTGTGCT AGCTTTCTGG AAACATGGAA 2581 TGCAAGGTAG AAGTTTTAGA TCTAATGAGG TAACACAAGA AATTTCAGAT AGCACAAGAA 2641 TTTTCAGGCT GCTTGGATCT GACAGGGTCG TGGTTTTGGA AAGTAGGCCA ACTGATAACC 2701 CCACAGCAAA TAGCAATTTG TACATCCTGG CGGGTCATGA AAACAGTTAC GACCCAGCTT 2761 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2821 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2881 CTTGTGGAAA GGACGAAAGA ATCCAGGAGA TGAAACCATC ACGCGTTAAG TCgacaatca 2941 acctctggat tacaaaattt gtgaaagatt