Construct: ORF TRCN0000491845
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017323.2_s317c1
- Derived from:
- ccsbBroadEn_06242
- DNA Barcode:
- TAGATACCCGGTCTCTAGAACCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GABRA6 (2559)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491845
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2559 | GABRA6 | gamma-aminobutyric acid typ... | NM_000811.3 | 99.8% | 100% | 951G>T;1344C>G |
| 2 | mouse | 14399 | Gabra6 | gamma-aminobutyric acid (GA... | NM_001099641.2 | 86.6% | 91.3% | (many diffs) |
| 3 | mouse | 14399 | Gabra6 | gamma-aminobutyric acid (GA... | NM_008068.3 | 84.5% | 89.1% | (many diffs) |
| 4 | mouse | 14399 | Gabra6 | gamma-aminobutyric acid (GA... | XM_006532197.3 | 84.1% | 89.6% | (many diffs) |
| 5 | mouse | 14399 | Gabra6 | gamma-aminobutyric acid (GA... | XM_006532198.3 | 70.6% | 74.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1428
- ORF length:
- 1359
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtcgtct ctgccctggc tgtgcattat tctgtggcta gaaaatgccc 121 tagggaaact cgaagttgaa ggcaacttct actcagaaaa cgtcagtcgg atcctggaca 181 acttgcttga aggctatgac aatcggctgc ggccgggatt tggaggtgct gtcactgaag 241 tcaaaacaga catttatgtg accagttttg ggcccgtgtc agatgtggag atggagtata 301 cgatggatgt ttttttccgc cagacctgga ctgatgagag gttgaagttt ggggggccaa 361 ctgagattct gagtctgaat aatttgatgg tcagtaaaat ctggacgcct gacacctttt 421 tcagaaatgg taaaaagtcc attgctcaca acatgacaac tcctaataaa ctcttcagaa 481 taatgcagaa tggaaccatt ttatacacca tgaggcttac catcaatgct gactgtccca 541 tgaggctggt taactttcct atggatgggc atgcttgtcc actcaagttt gggagctatg 601 cttatcccaa aagtgaaatc atatatacgt ggaaaaaagg accactttac tcagtagaag 661 tcccagaaga atcttcaagc cttctccagt atgatctgat tggacaaaca gtatctagtg 721 agacaattaa atctaacaca ggtgaatacg ttataatgac agtttacttc cacttgcaaa 781 ggaagatggg ctacttcatg atacagatat acactccttg cattatgaca gtcattcttt 841 cccaggtgtc tttctggatt aataaggagt ccgtcccagc aagaactgtt tttgggatca 901 ccactgtttt aactatgacc actttgagca tcagtgcccg gcactctttg ccaaaagtgt 961 catatgccac tgccatggat tggttcatag ctgtttgctt tgcattcgtc ttctctgctc 1021 ttatcgagtt cgcagctgtc aactacttta ccaatcttca gacacagaag gccaaaagga 1081 aggcacagtt tgcagcccca cccacagtga caatatcaaa agctactgaa cctttggaag 1141 ctgagattgt tttgcatcct gactccaaat atcatctgaa gaaaaggatc acttctctgt 1201 ctttgccaat agtttcatct tccgaggcca ataaagtgct cacgagagcg cccatcttac 1261 aatcaacacc tgtcacaccc ccaccactct cgccagcctt tGGAGGCACC AGTAAAATAG 1321 ACCAGTATTC TCGAATTCTC TTCCCAGTTG CATTTGCAGG ATTCAACCTT GTGTACTGGG 1381 TAGTTTATCT TTCCAAAGAT ACAATGGAAG TGAGTAGCAG TGTTGAATTG CCAACTTTCT 1441 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1501 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1561 GTGGAAAGGA CGATAGATAC CCGGTCTCTA GAACCTCACG CGTTAAGTCg acaatcaacc 1621 tctggattac aaaatttgtg aaagatt