Construct: ORF TRCN0000492109
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005002.1_s317c1
- Derived from:
- ccsbBroadEn_07248
- DNA Barcode:
- GCCTACGAGACGTCTGAGGCGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPFIA1 (8500)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492109
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | NM_003626.4 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_017018449.1 | 98.9% | 98.9% | (many diffs) |
| 3 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | NM_177423.2 | 98.3% | 98.2% | (many diffs) |
| 4 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545317.2 | 97.7% | 97.7% | (many diffs) |
| 5 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545319.1 | 97.2% | 97.2% | (many diffs) |
| 6 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545316.2 | 96.9% | 96.9% | (many diffs) |
| 7 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545315.2 | 96.5% | 96.5% | (many diffs) |
| 8 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545314.3 | 95.4% | 95.3% | (many diffs) |
| 9 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_017018448.1 | 94.7% | 94.7% | (many diffs) |
| 10 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545313.2 | 94.6% | 94.6% | (many diffs) |
| 11 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545312.2 | 94% | 93.9% | (many diffs) |
| 12 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545311.3 | 93.9% | 93.8% | (many diffs) |
| 13 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545310.3 | 93.6% | 93.5% | (many diffs) |
| 14 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545308.3 | 92.5% | 92.5% | (many diffs) |
| 15 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545307.3 | 92.4% | 92.4% | (many diffs) |
| 16 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_006718716.3 | 91.8% | 91.8% | (many diffs) |
| 17 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545306.3 | 91.8% | 91.8% | (many diffs) |
| 18 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545309.2 | 90.8% | 90.4% | (many diffs) |
| 19 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_017018450.1 | 78.8% | 73.8% | (many diffs) |
| 20 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | NR_045286.2 | 57.8% | (many diffs) | |
| 21 | human | 8500 | PPFIA1 | PTPRF interacting protein a... | XM_011545318.3 | 46% | 46% | (many diffs) |
| 22 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_006508610.2 | 85.7% | 95.5% | (many diffs) |
| 23 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_006508611.2 | 84.8% | 94.2% | (many diffs) |
| 24 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_006508609.3 | 83.9% | 93.6% | (many diffs) |
| 25 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_017322223.1 | 83.9% | 93.5% | (many diffs) |
| 26 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_017322222.1 | 83.7% | 93.2% | (many diffs) |
| 27 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_006508612.2 | 83.6% | 93.1% | (many diffs) |
| 28 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_006508608.3 | 83.3% | 92.8% | (many diffs) |
| 29 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_006508607.3 | 83.2% | 92.8% | (many diffs) |
| 30 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_017322224.1 | 83.1% | 92.3% | (many diffs) |
| 31 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | NM_001033319.2 | 83% | 92.5% | (many diffs) |
| 32 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_006508605.3 | 82.7% | 92.2% | (many diffs) |
| 33 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_006508603.3 | 82.6% | 92.1% | (many diffs) |
| 34 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_017322220.1 | 82% | 91.4% | (many diffs) |
| 35 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_006508601.3 | 81.9% | 91.3% | (many diffs) |
| 36 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_011241995.2 | 81.6% | 90.9% | (many diffs) |
| 37 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | NM_001195086.1 | 81.3% | 90.6% | (many diffs) |
| 38 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_006508599.3 | 81.3% | 90.6% | (many diffs) |
| 39 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_006508598.3 | 81.3% | 90.6% | (many diffs) |
| 40 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_006508604.3 | 81.1% | 90% | (many diffs) |
| 41 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_011241991.2 | 80.6% | 89.9% | (many diffs) |
| 42 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_017322221.1 | 80.4% | 89.3% | (many diffs) |
| 43 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_011241996.2 | 80% | 89.2% | (many diffs) |
| 44 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_011241992.2 | 79.2% | 87.9% | (many diffs) |
| 45 | mouse | 233977 | Ppfia1 | protein tyrosine phosphatas... | XM_011241997.2 | 39.6% | 43.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3672
- ORF length:
- 3606
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 taggcatgat gtgcgaggtg atgccgacca tcagcgaagc agaaggcccc cctggaggag 121 gtggaggcca tggttccggc tccccttcac agccagatgc agattcacat tttgaacagt 181 tgatggtctc catgctagaa gaaagggacc gccttcttga tacactgaga gagactcaag 241 aaacgctggc cttaacccag gggaagttac acgaggttgg tcatgaaaga gattccttgc 301 agagacagct caacacagca cttccacagg agttcgcagc acttactaaa gaactcaatg 361 tatgcaggga acagctcctt gaaagggaag aagaaattgc tgaactgaaa gcagaaagga 421 ataacaccag gctgctgtta gagcatttgg aatgccttgt ctccaggcat gagcggtctc 481 ttaggatgac cgtggtgaag agacaagcgc agtctccagc aggcgtgtcc agcgaagtgg 541 aagtgctgaa agcactgaag tccttatttg aacaccacaa agctctggat gaaaaggtga 601 gagagcgatt acgagtagca cttgaaagat gtagtttgtt agaagaggaa ttaggtgcca 661 cacacaaaga gctaatgatt cttaaagaac agaataatca gaaaaaaact ctaacagatg 721 gagtgctgga cataaaccat gaacaagaaa atacaccaag cacgagtgga aagagatctt 781 ctgatggttc tttaagccac gaggaagacc ttgctaaagt aattgagctc caagaaatca 841 taagtaagca gtcaagggaa cagagccaaa tgaaagaacg cctggcttcc ctttccagtc 901 atgtgacaga actggaagag gatctggaca cggctagaaa agatctcatc aaatctgaag 961 aaatgaacac aaaattgcaa cgagatgtcc gtgaagccat ggcccaaaag gaagatatgg 1021 aagagagaat cactactctt gaaaaacgct acctcgctgc acagcgtgaa gccacatctg 1081 tgcatgacct caatgataaa cttgaaaatg aaattgcaaa taaagattct atgcatcgac 1141 agactgaaga taaaaaccgc cagttacagg agcgcttgga attggcagag caaaagctgc 1201 aacagacact gaggaaggca gagacgctcc cggaggtgga ggcggagctg gcccagaggg 1261 tggcagcgct ttccaaggct gaagagagac acggcaacat tgaagaaagg ttacgacaga 1321 tggaagcaca gttggaggag aagaatcaag aactgcagcg ggcaaggcaa agagaaaaaa 1381 tgaacgaaga acataataaa cgtttatcag acactgttga caagctgctt tcagaatcta 1441 atgagaggct tcaacttcat cttaaagaga gaatggctgc tttggaagat aagaactctc 1501 ttttaagaga agttgaaagt gcaaaaaagc agttagaagg aacacaacac gataaggatc 1561 agcttgtcct aaacattgaa gcactgaggg ctgaactaga ccacatgaga ctaagaggtg 1621 cttcacttca tcatggccga ccccacttgg gcagtgtccc agatttcagg ttccccatgg 1681 cagatggcca cacagactcc tacagcacca gtgcagtgct gcggcgcaca cagaaaggcc 1741 ggctggcagc cctgcgagat gagccttcca aggtacaaac tcttaatgag caggattggg 1801 aacgtgccca gcaagctagt gtcttggcaa atgtagcaca agcattcgag agtgatgctg 1861 acgtgtctga tggtgaagat gacagggaca ctctcctcag ctcagttgac ctgctatcgc 1921 ccagcgggca ggccgacgcg cacacactag ccatgatgct tcaggagcag ctggacgcca 1981 tcaacaaaga gatcaggttg attcaggaag aaaaagaaaa tacagagcag cgggcagagg 2041 agattgaaag tcgagttggc agtggaagtc tagacaatct tggtcgtttt agatcaatga 2101 gctccattcc cccctaccct gcttcctcgc ttgctagctc ctcccctccg ggcagtgggc 2161 gctccacccc acgaaggatc cctcacagcc cagctcggga agtggacaga ctgggcgtca 2221 tgaccctttt gccaccttcc agagaagagg tacgagatga caagacaacc ataaagtgtg 2281 aaacctcccc gccttcctcc ccgagagccc ttcggttaga ccggctgcac aaaggggcgc 2341 tgcacaccgt tagccacgag gacatcaggg acataaggaa ctccacaggc tcccaggatg 2401 gtcccgtgag caaccccagc agtagcaaca gtagccagga ctcgctccac aaagccccaa 2461 agaagaaagg cattaagtcc tccattggcc gcttgtttgg caagaaagaa aagggccgac 2521 ctggacaaac tggcaaagaa gcattaggac aagctggtgt ttccgagacg gataactcat 2581 ctcaggatgc cttgggactt agcaaattgg ggggacaggc tgaaaaaaat cgtaaacttc 2641 aaaaaaagca tgaattgctg ggggaagccc ggagacaagg tttacctttt gcccaatggg 2701 acgggccaac ggttgtggtc tggctagagc tctgggttgg gatgccagcc tggtatgtgg 2761 ctgcctgccg agcaaacgtg aaaagcgggg ccatcatgtc ggccctgtcc gacacagaga 2821 tccagcgtga gattggcatc agcaaccccc tgcacaggct gaagctgagg ctggccatcc 2881 aggagatcat gtcgctgacc agcccgtctg ccccgcccac atctagaacg acactcgcct 2941 atggggacat gaaccacgag tggatcggca acgagtggct ccccagcctg ggcctccccc 3001 agtaccgcag ctacttcatg gagtgccttg tagacgccag gatgctggac cacttgacca 3061 agaaagacct tcgagggcag ctgaaaatgg tcgacagttt tcacagaaac agtttccagt 3121 gtggaattat gtgcctgaga aggttaaatt atgaccggaa agaactggaa agaaaaagag 3181 aagaaagtca gagtgaaata aaagacgtgc ttgtttggag caatgatcga gtgattcgct 3241 ggatcctgtc aattggcctt aaagaatatg caaacaatct tatagagagt ggtgttcacg 3301 gagcacttct ggccttagat gaaaccTTCG ACTTCAGTGC ACTGGCACTG CTGTTACAGA 3361 TCCCAACGCA GAACACACAG GCTCGTGCTG TCTTGGAAAG AGAATTTAAC AACCTTTTGG 3421 TCATGGGGAC TGATAGAAGG TTTGATGAAG ATGATGATAA AAGCTTTAGG AGAGCACCTT 3481 CATGGAGAAA AAAGTTTAGA CCAAAGGACA TTCGTGGCTT AGCTGCTGGG TCAGCAGAGA 3541 CTCTCCCTGC AAACTTCCGG GTGACTTCTT CTATGTCTTC CCCCTCTATG CAGCCAAAGA 3601 AGATGCAGAT GGACGGCAAT GTATCAGGAA CACAGAGGTT GGATTCTGCT ACAGTCAGGA 3661 CTTACTCCTG CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 3721 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 3781 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCC TACGAGACGT CTGAGGCGCC 3841 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t