Construct: ORF TRCN0000492337
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019512.2_s317c1
- DNA Barcode:
- ACACGTCCGAGTCATCCTGCGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TNIK (23043)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492337
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23043 | TNIK | TRAF2 and NCK interacting k... | NM_001161561.3 | 99.9% | 100% | 2244G>C |
| 2 | human | 23043 | TNIK | TRAF2 and NCK interacting k... | NM_001161562.3 | 99.3% | 99.3% | 2244G>C;2295_2296ins24 |
| 3 | human | 23043 | TNIK | TRAF2 and NCK interacting k... | NM_015028.4 | 97.8% | 97.8% | 1332_1418del;2331G>C |
| 4 | human | 23043 | TNIK | TRAF2 and NCK interacting k... | NM_001161560.3 | 97.2% | 97.2% | 1332_1418del;2331G>C;2382_2383ins24 |
| 5 | human | 23043 | TNIK | TRAF2 and NCK interacting k... | NM_001161565.3 | 95.8% | 95.8% | 1519_1520ins165;2079G>C |
| 6 | human | 23043 | TNIK | TRAF2 and NCK interacting k... | NM_001161566.3 | 95.2% | 95.2% | 1519_1520ins165;2079G>C;2130_2131ins24 |
| 7 | human | 23043 | TNIK | TRAF2 and NCK interacting k... | NM_001161563.3 | 93.7% | 93.8% | 1332_1418del;1606_1607ins165;2166G>C |
| 8 | human | 23043 | TNIK | TRAF2 and NCK interacting k... | NM_001161564.3 | 93.2% | 93.2% | (many diffs) |
| 9 | mouse | 665113 | Tnik | TRAF2 and NCK interacting k... | XM_006535521.2 | 91% | 98.7% | (many diffs) |
| 10 | mouse | 665113 | Tnik | TRAF2 and NCK interacting k... | NM_001163008.1 | 90.5% | 98.1% | (many diffs) |
| 11 | mouse | 665113 | Tnik | TRAF2 and NCK interacting k... | XM_006535520.2 | 89.1% | 95% | (many diffs) |
| 12 | mouse | 665113 | Tnik | TRAF2 and NCK interacting k... | NM_026910.1 | 89.1% | 96.6% | (many diffs) |
| 13 | mouse | 665113 | Tnik | TRAF2 and NCK interacting k... | XM_006535519.2 | 88.9% | 96.4% | (many diffs) |
| 14 | mouse | 665113 | Tnik | TRAF2 and NCK interacting k... | NM_001163007.1 | 88.5% | 96.1% | (many diffs) |
| 15 | mouse | 665113 | Tnik | TRAF2 and NCK interacting k... | XM_006535518.2 | 87.4% | 93.2% | (many diffs) |
| 16 | mouse | 665113 | Tnik | TRAF2 and NCK interacting k... | XM_006535517.2 | 87.2% | 93% | (many diffs) |
| 17 | mouse | 665113 | Tnik | TRAF2 and NCK interacting k... | NM_001163009.1 | 86.9% | 92.7% | (many diffs) |
| 18 | mouse | 665113 | Tnik | TRAF2 and NCK interacting k... | XM_011249705.2 | 85.4% | 91% | (many diffs) |
| 19 | mouse | 665113 | Tnik | TRAF2 and NCK interacting k... | XM_011249704.2 | 83.6% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 87
- ORF end:
- 4080
- ORF length:
- 3993
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcacctct agagccatgg cgagcgactc cccggctcga agcctggatg 121 aaatagatct ctcggctctg agggaccccg cagggatctt tgaattggtg gaacttgttg 181 gaaatggaac atacgggcaa gtttataagg gtcgtcatgt caaaacgggc cagcttgcag 241 ccatcaaggt tatggatgtc acaggggatg aagaggaaga aatcaaacaa gaaattaaca 301 tgttgaagaa atattctcat caccggaata ttgctacata ctatggtgct tttatcaaaa 361 agaacccacc aggcatggat gaccaacttt ggttggtgat ggagttttgt ggtgctggct 421 ctgtcaccga cctgatcaag aacacaaaag gtaacacgtt gaaagaggag tggattgcat 481 acatctgcag ggaaatctta cgggggctga gtcacctgca ccagcataaa gtgattcatc 541 gagatattaa agggcaaaat gtcttgctga ctgaaaatgc agaagttaaa ctagtggact 601 ttggagtcag tgctcagctt gatcgaacag tgggcaggag gaatactttc attggaactc 661 cctactggat ggcaccagaa gttattgcct gtgatgaaaa cccagatgcc acatatgatt 721 tcaagagtga cttgtggtct ttgggtatca ccgccattga aatggcagaa ggtgctcccc 781 ctctctgtga catgcacccc atgagagctc tcttcctcat cccccggaac ccagcgcctc 841 ggctgaagtc taagaagtgg tcaaaaaaat tccagtcatt tattgagagc tgcttggtaa 901 agaatcacag ccagcgacca gcaacagaac aattgatgaa gcatccattt atacgagacc 961 aacctaatga gcgacaggtc cgcattcaac tcaaggacca tattgataga acaaagaaga 1021 agcgaggaga aaaagatgag acagagtatg agtacagtgg aagtgaggaa gaagaggagg 1081 agaatgactc aggagagccc agctccatcc tgaatctgcc aggggagtcg acgctgcgga 1141 gggactttct gaggctgcag ctggccaaca aggagcgttc tgaggcccta cggaggcagc 1201 agctggagca gcagcagcgg gagaatgagg agcacaagcg gcagctgctg gccgagcgtc 1261 agaagcgcat cgaggagcag aaagagcaga ggcggcggct ggaggagcaa caaaggcgag 1321 agaaggagct gcggaagcag caggagaggg agcagcgccg gcactatgag gagcagatgc 1381 gccgggagga ggagaggagg cgtgcggagc atgaacagga atataagcgc aaacaattgg 1441 aagaacagag acaagcagaa agactgcaga ggcagctaaa gcaagaaaga gactacttag 1501 tttcccttca gcatcagcgg caggagcaga ggcctgtgga gaagaagcca ctgtaccatt 1561 acaaagaagg aatgagtcct agtgagaagc cagcatgggc caaggaggta gaagaacggt 1621 caaggctcaa ccggcaaagt tcccctgcca tgcctcacaa ggttgccaac aggatatctg 1681 accccaacct gcccccaagg tcggagtcct tcagcattag tggagttcag cctgctcgaa 1741 caccccccat gctcagacca gtcgatcccc agatcccaca tctggtagct gtaaaatccc 1801 agggacctgc cttgaccgcc tcccagtcag tgcacgagca gcccacaaag ggcctctctg 1861 ggtttcagga ggctctgaac gtgacctccc accgcgtgga gatgccacgc cagaactcag 1921 atcccacctc ggaaaatcct cctctcccca ctcgcattga aaagtttgac cgaagctctt 1981 ggttacgaca ggaagaagac attccaccaa aggtgcctca aagaacaact tctatatccc 2041 cagcattagc cagaaagaat tctcctggga atggtagtgc tctgggaccc agactaggat 2101 ctcaacccat cagagcaagc aaccctgatc tccggagaac tgagcccatc ttggagagcc 2161 ccttgcagag gaccagcagt ggcagttcct ccagctccag cacccctagc tcccagccca 2221 gctcccaagg aggctcccag cctggatcac aagcaggatc cagtgaacgc accagagttc 2281 gagccaacag taagtcagaa ggatcacctg tgctccccca tgagcctgcc aaggtgaaac 2341 cagaagaatc cagggacatt acccggccca gtcgaccagc tagctacaaa aaagctatag 2401 atgaggatct gacggcatta gccaaagaac taagagaact ccggattgaa gaaacaaacc 2461 gcccaatgaa gaaggtgact gattactcct cctccagtga ggagtcagaa agtagcgagg 2521 aagaggagga agatggagag agcgagaccc atgatgggac agtggctgtc agcgacatac 2581 ccagactgat accaacagga gctccaggca gcaacgagca gtacaatgtg ggaatggtgg 2641 ggacgcatgg gctggagacc tctcatgcgg acagtttcag cggcagtatt tcaagagaag 2701 gaaccttgat gattagagag acgtctggag agaagaagcg atctggccac agtgacagca 2761 atggctttgc tggccacatc aacctccctg acctggtgca gcagagccat tctccagctg 2821 gaaccccgac tgagggactg gggcgcgtct caacccattc ccaggagatg gactctggga 2881 ctgaatatgg catggggagc agcaccaaag cctccttcac cccctttgtg gaccccagag 2941 tataccagac gtctcccact gatgaagatg aagaggatga ggaatcatca gccgcagctc 3001 tgtttactag cgaacttctt aggcaagaac aggccaaact caatgaagca agaaagattt 3061 cggtggtaaa tgtaaaccca accaacattc ggcctcatag cgacacacca gaaatcagaa 3121 aatacaagaa acgattcaac tcagaaatac tttgtgcagc tctgtggggt gtaaaccttc 3181 tggtggggac tgaaaatggc ctgatgcttt tggaccgaag tgggcaaggc aaagtctata 3241 atctgatcaa ccggaggcga tttcagcaga tggatgtgct agagggactg aatgtccttg 3301 tgacaatttc aggaaagaag aataagctac gagtttacta tctttcatgg ttaagaaaca 3361 gaatactaca taatgaccca gaagtagaaa agaaacaagg ctggatcact gttggggact 3421 tggaaggctg tatacattat aaagttgtta aatatgaaag gatcaaattt ttggtgattg 3481 ccttaaagaa tgctgtggaa atatatgctt gggctcctaa accgtatcat aaattcatgg 3541 catttaagtc ttttgcagat ctccagcaca agcctctgct agttgatctc acggtagaag 3601 aaggtcaaag attaaaggtt atttttggtt cacacactgg tttccatgta attgatgttg 3661 attcaggaaa ctcttatgat atctacatac catctcatat tcagggcaat atcactcctc 3721 atgctattgt catcttgcct aaaacagatG GAATGGAAAT GCTTGTTTGC TATGAGGATG 3781 AGGGGGTGTA TGTAAACACC TATGGCCGGA TAACTAAGGA TGTGGTGCTC CAATGGGGAG 3841 AAATGCCCAC GTCTGTGGCC TACATTCATT CCAATCAGAT AATGGGCTGG GGCGAGAAAG 3901 CTATTGAGAT CCGGTCAGTG GAAACAGGAC ATTTGGATGG AGTATTTATG CATAAGCGAG 3961 CTCAAAGGTT AAAGTTTCTA TGTGAAAGAA ATGATAAGGT ATTTTTTGCA TCCGTGCGAT 4021 CTGGAGGAAG TAGCCAAGTG TTTTTCATGA CCCTCAACAG AAATTCCATG ATGAACTGGA 4081 AGGGTGGGCG CGCCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 4141 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA TTGAAAGTAT 4201 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CACGTCCGAG TCATCCTGCG 4261 GACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att