Construct: ORF ccsbBroad304_00841

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004983.1_s304c1, BRDN0000461320
Derived from:
ccsbBroadEn_00841
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IKBKB (3551)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00841
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3551 IKBKB inhibitor of nuclear factor... XM_005273494.3 58.6% 57% (many diffs)
2 human 3551 IKBKB inhibitor of nuclear factor... XM_005273492.4 56% 54.4% (many diffs)
3 human 3551 IKBKB inhibitor of nuclear factor... NM_001556.3 52.3% 50.8% (many diffs)
4 human 3551 IKBKB inhibitor of nuclear factor... XM_005273490.3 49.2% 47.7% (many diffs)
5 human 3551 IKBKB inhibitor of nuclear factor... XM_011544517.2 49.2% 47.7% (many diffs)
6 human 3551 IKBKB inhibitor of nuclear factor... NM_001190720.2 48.6% 45.9% (many diffs)
7 human 3551 IKBKB inhibitor of nuclear factor... NM_001242778.2 44.6% 42.1% (many diffs)
8 human 3551 IKBKB inhibitor of nuclear factor... XM_011544518.2 43.1% 39.5% (many diffs)
9 human 3551 IKBKB inhibitor of nuclear factor... XM_005273491.5 42% 39.6% (many diffs)
10 human 3551 IKBKB inhibitor of nuclear factor... XM_011544519.2 42% 39.6% (many diffs)
11 human 3551 IKBKB inhibitor of nuclear factor... XM_011544520.2 37.2% 31.5% (many diffs)
12 human 3551 IKBKB inhibitor of nuclear factor... XM_005273493.4 36.3% 32% (many diffs)
13 human 3551 IKBKB inhibitor of nuclear factor... XR_001745530.2 31.6% (many diffs)
14 human 3551 IKBKB inhibitor of nuclear factor... NR_040009.2 31.5% (many diffs)
15 human 3551 IKBKB inhibitor of nuclear factor... NR_033819.2 30.8% (many diffs)
16 human 3551 IKBKB inhibitor of nuclear factor... NR_033818.2 29.9% (many diffs)
17 human 3551 IKBKB inhibitor of nuclear factor... XR_949402.3 24.8% (many diffs)
18 human 3551 IKBKB inhibitor of nuclear factor... XM_005273495.2 23.4% 19.3% (many diffs)
19 human 3551 IKBKB inhibitor of nuclear factor... XM_005273496.4 23.4% 19.3% (many diffs)
20 human 3551 IKBKB inhibitor of nuclear factor... XM_005273498.4 23.4% 19.3% (many diffs)
21 human 3551 IKBKB inhibitor of nuclear factor... XM_011544521.2 23.4% 19.3% (many diffs)
22 mouse 16150 Ikbkb inhibitor of kappaB kinase ... XM_006509023.3 81.6% 84.4% (many diffs)
23 mouse 16150 Ikbkb inhibitor of kappaB kinase ... XM_006509022.3 66.7% 68.4% (many diffs)
24 mouse 16150 Ikbkb inhibitor of kappaB kinase ... XM_006509021.2 64.8% 66.5% (many diffs)
25 mouse 16150 Ikbkb inhibitor of kappaB kinase ... XM_006509020.3 50.1% 51.2% (many diffs)
26 mouse 16150 Ikbkb inhibitor of kappaB kinase ... NM_010546.2 47.9% 48.9% (many diffs)
27 mouse 16150 Ikbkb inhibitor of kappaB kinase ... XM_006509019.3 47.6% 48.6% (many diffs)
28 mouse 16150 Ikbkb inhibitor of kappaB kinase ... NM_001159774.1 46.7% 47.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1329
ORF length:
1260
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gagctggtca ccttccctga caacgcagac atgtggggcc tgggaaatga
121aagagcgcct tgggacaggg ggatttggaa atgtcatccg atggcacaat caggaaacag
181gtgagcagat tgccatcaag cagtgccggc aggagctcag cccccggaac cgagagcggt
241ggtgcctgga gatccagatc atgagaaggc tgacccaccc caatgtggtg gctgcccgag
301atgtccctga ggggatgcag aacttggcgc ccaatgacct gcccctgctg gccatggagt
361actgccaagg aggagatctc cggaagtacc tgaaccagtt tgagaactgc tgtggtctgc
421gggaaggtgc catcctcacc ttgctgagtg acattgcctc tgcgcttaga taccttcatg
481aaaacagaat catccatcgg gatctaaagc cagaaaacat cgtcctgcag caaggagaac
541agaggttaat acacaaaatt attgacctag gatatgccaa ggagctggat cagggcagtc
601tttgcacatc attcgtgggg accctgcagt acctggcccc agagctactg gagcAGCAGA
661AGTACACAGT GACCGTCGAC TACTGGAGCT TCGGCACCCT GGCCTTTGAG TGCATCACGG
721GCTTCCGGCC CTTCCTCCCC AACTGGCAGC CCGTGCAGTG GCATTCAAAA GTGCGGCAGA
781AGAGTGAGGT GGACATTGTT GTTAGCGAAG ACTTGAATGG AACGGTGAAG TTTTCAAGCT
841CTTTACCCTA CCCCAATAAT CTTAACAGTG TCCTGGCTGA GCGACTGGAG AAGTGGCTGC
901AACTGATGCT GATGTGGCAC CCCCGACAGA GGGGCACGGA TCCCACGTAT GGGCCCAATG
961GCTGCTTCAA GGCCCTGGAT GACATCTTAA ACTTAAAGCT GGTTCATATC TTGAACATGG
1021TCACGGGCAC CATCCACACC TACCCTGTGA CAGAGGATGA GAGTCTGCAG AGCTTGAAGG
1081CCAGAATCCA ACAGGACACG GGCATCCCAG AGGAGGACCA GGAGCTGCTG CAGGAAGCGG
1141GCCTGGCGTT GATCCCCGAT AAGCCTGCCA CTCAGTGTAT TTCAGACGGC AAGGTGAGCC
1201CTGGCTTCGT ACACACCATC CTGTTTACCT TGGCTGTGCC TCCTGGGAAA CTCAACACAC
1261TTTCAGATTT CAATTCTGCT TTGTCATGTA GTCTGTTAAT CACACAGTGC GGATACCTGG
1321CTGGTTTTTT GCCAACTTTC TTGTACaaag tggttggtaa gcctatccct aaccctctcc
1381tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
1441tcaacctctg gatta

Download FASTA (ORF) (Full)