Construct: ORF ccsbBroad304_01440
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015996.1_s304c1, BRDN0000408127
- Derived from:
- ccsbBroadEn_01440
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RPL39 (6170)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01440
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6170 | RPL39 | ribosomal protein L39 | NM_001000.4 | 100% | 100% | |
| 2 | human | 116832 | RPL39L | ribosomal protein L39 like | NM_052969.3 | 94.7% | 92.1% | (many diffs) |
| 3 | human | 170958 | ZNF525 | zinc finger protein 525 | NR_145447.2 | 7.5% | (many diffs) | |
| 4 | human | 170958 | ZNF525 | zinc finger protein 525 | NR_145446.2 | 6.6% | (many diffs) | |
| 5 | human | 388558 | ZNF808 | zinc finger protein 808 | XR_935832.3 | 3.3% | (many diffs) | |
| 6 | human | 388558 | ZNF808 | zinc finger protein 808 | XR_002958314.1 | 3.1% | (many diffs) | |
| 7 | human | 170958 | ZNF525 | zinc finger protein 525 | NR_145445.2 | 2.7% | (many diffs) | |
| 8 | mouse | 68172 | Rpl39l | ribosomal protein L39-like | NM_026594.2 | 91.5% | 94.1% | (many diffs) |
| 9 | mouse | 68172 | Rpl39l | ribosomal protein L39-like | XM_006522506.3 | 91.5% | 94.1% | (many diffs) |
| 10 | mouse | 67248 | Rpl39 | ribosomal protein L39 | NM_026055.2 | 89.5% | 100% | (many diffs) |
| 11 | mouse | 102634812 | LOC102634812 | ribosomal protein L39 pseud... | NR_136931.1 | 7.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 219
- ORF length:
- 153
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaacc 61 tcgccatgtc ttctcacaag actttcagga ttaagcgatt cctggccaag aaacaaaagc 121 aaaatcgtcc cattccccag tggattcgga tgaaaactgg aaataaaatc aggtacaact 181 ccaaaaggag acattggaga agaaccaagc tgggtctata aggaactttc ttgtacaaag 241 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 301 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta