Construct: ORF ccsbBroad304_01896
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017555.1_s304c1, BRDN0000410805
- Derived from:
- ccsbBroadEn_01896
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2AC20 (8338)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01896
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8338 | H2AC20 | H2A clustered histone 20 | NM_003517.2 | 100% | 100% | |
| 2 | human | 723790 | H2AC19 | H2A clustered histone 19 | NM_001040874.1 | 94.3% | 98.4% | (many diffs) |
| 3 | human | 8337 | H2AC18 | H2A clustered histone 18 | NM_003516.2 | 94.3% | 98.4% | (many diffs) |
| 4 | human | 317772 | H2AC21 | H2A clustered histone 21 | NM_175065.2 | 85.4% | 95.3% | (many diffs) |
| 5 | human | 8329 | H2AC13 | H2A clustered histone 13 | NM_003509.2 | 85.1% | 96.9% | (many diffs) |
| 6 | human | 8331 | H2AC14 | H2A clustered histone 14 | NM_021066.2 | 85% | 96.1% | (many diffs) |
| 7 | human | 8334 | H2AC6 | H2A clustered histone 6 | NM_003512.3 | 84.6% | 96.9% | (many diffs) |
| 8 | human | 8336 | H2AC17 | H2A clustered histone 17 | NM_003514.2 | 84.6% | 96.9% | (many diffs) |
| 9 | human | 8969 | H2AC11 | H2A clustered histone 11 | NM_021064.4 | 84.6% | 96.9% | (many diffs) |
| 10 | human | 8332 | H2AC16 | H2A clustered histone 16 | NM_003511.2 | 84.1% | 96.9% | (many diffs) |
| 11 | human | 92815 | H2AW | H2A.W histone | NM_033445.2 | 84.1% | 96.1% | (many diffs) |
| 12 | human | 85235 | H2AC12 | H2A clustered histone 12 | NM_080596.2 | 83.3% | 96.1% | (many diffs) |
| 13 | human | 3013 | H2AC7 | H2A clustered histone 7 | NM_021065.3 | 83% | 96.1% | (many diffs) |
| 14 | human | 55766 | H2AJ | H2A.J histone | NM_177925.3 | 82.6% | 96.8% | (many diffs) |
| 15 | human | 8330 | H2AC15 | H2A clustered histone 15 | NM_003510.2 | 82.3% | 96.9% | (many diffs) |
| 16 | human | 8335 | H2AC4 | H2A clustered histone 4 | NM_003513.2 | 81.2% | 95.3% | (many diffs) |
| 17 | human | 3014 | H2AX | H2A.X variant histone | NM_002105.2 | 75.6% | 83.9% | (many diffs) |
| 18 | mouse | 319176 | Hist2h2ac | histone cluster 2, H2ac | NM_175662.2 | 88.3% | 100% | (many diffs) |
| 19 | mouse | 15267 | Hist2h2aa1 | histone cluster 2, H2aa1 | NM_013549.2 | 87.4% | 98.4% | (many diffs) |
| 20 | mouse | 319192 | Hist2h2aa2 | histone cluster 2, H2aa2 | NM_178212.3 | 87.4% | 98.4% | (many diffs) |
| 21 | mouse | 319167 | Hist1h2ag | histone cluster 1, H2ag | NM_178186.3 | 85.3% | 95.3% | (many diffs) |
| 22 | mouse | 319166 | Hist1h2ae | histone cluster 1, H2ae | NM_178187.4 | 85.3% | 95.3% | (many diffs) |
| 23 | mouse | 232440 | H2afj | H2A histone family, member J | NM_177688.4 | 85.2% | 96.1% | (many diffs) |
| 24 | mouse | 665433 | Hist1h2ao | histone cluster 1, H2ao | NM_001177544.2 | 85.1% | 95.3% | (many diffs) |
| 25 | mouse | 319170 | Hist1h2an | histone cluster 1, H2an | NM_178184.2 | 85.1% | 95.3% | (many diffs) |
| 26 | mouse | 319171 | Hist1h2ap | histone cluster 1, H2ap | NM_178185.2 | 85.1% | 95.3% | (many diffs) |
| 27 | mouse | 319165 | Hist1h2ad | histone cluster 1, H2ad | NM_178188.4 | 85.1% | 95.3% | (many diffs) |
| 28 | mouse | 319164 | Hist1h2ac | histone cluster 1, H2ac | NM_178189.5 | 84.8% | 95.3% | (many diffs) |
| 29 | mouse | 319173 | Hist1h2af | histone cluster 1, H2af | NM_175661.2 | 84.8% | 94.6% | (many diffs) |
| 30 | mouse | 319191 | Hist1h2ai | histone cluster 1, H2ai | NM_178182.2 | 83.7% | 95.3% | (many diffs) |
| 31 | mouse | 319168 | Hist1h2ah | histone cluster 1, H2ah | NM_175659.2 | 83.5% | 94.6% | (many diffs) |
| 32 | mouse | 319172 | Hist1h2ab | histone cluster 1, H2ab | NM_175660.3 | 83.5% | 95.3% | (many diffs) |
| 33 | mouse | 621893 | Hist2h2ab | histone cluster 2, H2ab | NM_178213.4 | 83.3% | 96.1% | (many diffs) |
| 34 | mouse | 319169 | Hist1h2ak | histone cluster 1, H2ak | NM_178183.2 | 82.7% | 94.6% | (many diffs) |
| 35 | mouse | 15270 | H2afx | H2A histone family, member X | NM_010436.2 | 75.9% | 83.2% | (many diffs) |
| 36 | mouse | 667728 | Hist1h2al | histone cluster 1, H2al | NR_132443.1 | 10.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 453
- ORF length:
- 387
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tggtcgtggc aaacaaggag gcaaggcccg cgccaaggcc aagtcgcgct 121 cgtcccgcgc tggcctccag ttcccggtag ggcgagtgca ccgcttgctg cgcaaaggca 181 actacgcgga gcgggtgggg gccggcgcgc ccgtctacat ggcggcggtc ctcgagtacc 241 tgaccgccga gatcctggag ctggcgggca acgcggctcg ggacaacaag aagacgcgca 301 tcatccctcg tcacctccag ctggccatcc gcaacgacga ggaactgaac aagctgctgg 361 gcaaagtcac catcgcccag ggcggcgttt tgcctaacat ccaggccgtt ctgttaccaa 421 agaaaaccga aagccacaaa gccaaaagca aatacccaac tttcttgtac aaagtggttg 481 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 541 accggtggcg cgttaagtcg acaatcaacc tctggatta