Transcript: Mouse NR_132443.1

Mus musculus histone cluster 1, H2al (Hist1h2al), non-coding RNA.

Source:
NCBI, updated 2015-10-06
Taxon:
Mus musculus (mouse)
Gene:
Hist1h2al (667728)
Length:
2983
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_132443.1
NBCI Gene record:
Hist1h2al (667728)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NR_132443.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000106658 CAACAAGAAGACTCGCATCAT pLKO.1 205 3UTR 100% 4.950 2.970 N H2AC14 n/a
2 TRCN0000074574 CGACAACAAGAAGACTCGCAT pLKO.1 202 3UTR 100% 2.640 1.584 N H2AC6 n/a
3 TRCN0000300638 CGACAACAAGAAGACTCGCAT pLKO_005 202 3UTR 100% 2.640 1.584 N H2AC6 n/a
4 TRCN0000096996 GAAGACGGAGAGCCACCATAA pLKO.1 343 3UTR 100% 10.800 5.400 Y Hist2h2aa1 n/a
5 TRCN0000242133 CAACGACGAGGAGCTCAACAA pLKO_005 253 3UTR 100% 4.950 2.475 Y Hist1h2ag n/a
6 TRCN0000364413 CAACGACGAGGAGCTCAACAA pLKO_005 253 3UTR 100% 4.950 2.475 Y H2AX n/a
7 TRCN0000096995 CTCAACAAGCTGCTGGGCAAA pLKO.1 266 3UTR 100% 4.050 2.025 Y Hist2h2aa1 n/a
8 TRCN0000369835 GCAACGACGAGGAGCTCAACA pLKO_005 252 3UTR 100% 1.650 0.825 Y H2AC17 n/a
9 TRCN0000255256 GGAGTACCTAACGGCCGAGAT pLKO_005 154 3UTR 100% 1.350 0.675 Y Hist2h2ab n/a
10 TRCN0000433810 CGACGAGGAGCTCAACAAGCT pLKO_005 256 3UTR 100% 0.880 0.440 Y H2AW n/a
11 TRCN0000096994 CCACCATAAGGCGAAGGGCAA pLKO.1 355 3UTR 100% 0.720 0.360 Y Hist2h2aa1 n/a
12 TRCN0000097023 CGAGGAGCTCAACAAGCTGCT pLKO.1 259 3UTR 100% 0.720 0.360 Y Hist1h2ae n/a
13 TRCN0000096998 GCTCAACAAGCTGCTGGGCAA pLKO.1 265 3UTR 100% 0.720 0.360 Y Hist2h2aa1 n/a
14 TRCN0000097003 TGGAGTACCTAACGGCCGAGA pLKO.1 153 3UTR 100% 0.720 0.360 Y Hist2h2ac n/a
15 TRCN0000256452 ACGGCCGAGATCCTGGAGCTG pLKO_005 164 3UTR 100% 0.000 0.000 Y Hist1h2ai n/a
16 TRCN0000242134 CAACATCCAGGCCGTGCTGCT pLKO_005 316 3UTR 100% 0.000 0.000 Y Hist1h2ag n/a
17 TRCN0000092688 CCAAGGCCAAGTCGCGGTCTT pLKO.1 24 3UTR 100% 0.000 0.000 Y Hist2h2aa2 n/a
18 TRCN0000097030 CCGCAACGACGAGGAGCTCAA pLKO.1 250 3UTR 100% 0.000 0.000 Y Hist1h2ab n/a
19 TRCN0000097032 CCTGCAGCTGGCCATCCGCAA pLKO.1 235 3UTR 100% 0.000 0.000 Y Hist1h2ab n/a
20 TRCN0000073282 CCTGCCCAACATCCAGGCCGT pLKO.1 310 3UTR 100% 0.000 0.000 Y H2AX n/a
21 TRCN0000092690 CGGCGGTGCTGGAGTACCTAA pLKO.1 144 3UTR 100% 0.000 0.000 Y Hist2h2aa2 n/a
22 TRCN0000242131 GCAGCTGGCCATCCGCAACGA pLKO_005 238 3UTR 100% 0.000 0.000 Y Hist1h2ag n/a
23 TRCN0000096999 GCCAAGGCCAAGTCGCGGTCT pLKO.1 23 3UTR 100% 0.000 0.000 Y Hist2h2ac n/a
24 TRCN0000096997 GCCCAAGAAGACGGAGAGCCA pLKO.1 337 3UTR 100% 0.000 0.000 Y Hist2h2aa1 n/a
25 TRCN0000097002 GCGCAAGGGCAACTACGCGGA pLKO.1 91 3UTR 100% 0.000 0.000 Y Hist2h2ac n/a
26 TRCN0000092691 GCTGCTGCGCAAGGGCAACTA pLKO.1 85 3UTR 100% 0.000 0.000 Y Hist2h2aa2 n/a
27 TRCN0000097000 GCTGGAGTACCTAACGGCCGA pLKO.1 151 3UTR 100% 0.000 0.000 Y Hist2h2ac n/a
28 TRCN0000097031 GCTGGCCATCCGCAACGACGA pLKO.1 241 3UTR 100% 0.000 0.000 Y Hist1h2ab n/a
29 TRCN0000255259 TGCTGCGCAAGGGCAACTACG pLKO_005 87 3UTR 100% 0.000 0.000 Y Hist2h2ab n/a
30 TRCN0000267543 TGTGCACCGGCTGCTGCGCAA pLKO_005 76 3UTR 100% 0.000 0.000 Y Hist2h2ab n/a
31 TRCN0000255946 CCCGCGACAACAAGAAGACGC pLKO_005 198 3UTR 100% 0.000 0.000 Y Hist1h2ah n/a
32 TRCN0000074592 GCCATCCGCAACGACGAGGAA pLKO.1 245 3UTR 100% 0.000 0.000 Y H2AC18 n/a
33 TRCN0000106666 GCCGAGATCCTGGAGCTGGCT pLKO.1 167 3UTR 100% 0.000 0.000 Y H2AC13 n/a
34 TRCN0000368904 TGCTGCTGCCCAAGAAGACCA pLKO_005 330 3UTR 100% 0.880 0.440 Y H2AX n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_132443.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00717 pDONR223 100% 12.2% None (many diffs) n/a
2 ccsbBroad304_00717 pLX_304 0% 12.2% V5 (many diffs) n/a
3 TRCN0000469289 TCCAAGCCCTCATTGGAGCTGGTC pLX_317 100% 12.2% V5 (many diffs) n/a
4 ccsbBroadEn_01891 pDONR223 100% 11.2% None (many diffs) n/a
5 ccsbBroad304_01891 pLX_304 0% 11.2% V5 (many diffs) n/a
6 TRCN0000481190 CGTGTTGGCCGTTCGATTTAGAAT pLX_317 98.5% 11.2% V5 (many diffs) n/a
7 ccsbBroadEn_04591 pDONR223 100% 11.2% None (many diffs) n/a
8 ccsbBroad304_04591 pLX_304 0% 11.2% V5 (many diffs) n/a
9 TRCN0000472223 CCTTGCTACGATGTATGAGACTTA pLX_317 100% 11.2% V5 (many diffs) n/a
10 ccsbBroadEn_15630 pDONR223 0% 11.1% None (many diffs) n/a
11 ccsbBroad304_15630 pLX_304 0% 11.1% V5 (many diffs) n/a
12 TRCN0000466536 CGATTGTCATTCGGCCGACTATTC pLX_317 51.9% 11.1% V5 (many diffs) n/a
13 ccsbBroadEn_02055 pDONR223 100% 11.1% None (many diffs) n/a
14 ccsbBroad304_02055 pLX_304 0% 11.1% V5 (many diffs) n/a
15 TRCN0000466333 TGGGGTTTAATTGCATTCTTGTTA pLX_317 72.6% 11.1% V5 (many diffs) n/a
16 ccsbBroadEn_01893 pDONR223 100% 11.1% None (many diffs) n/a
17 ccsbBroad304_01893 pLX_304 97.8% 11.1% V5 (many diffs) n/a
18 TRCN0000469277 CTTGTACACGCATGGACCTTCTTT pLX_317 100% 11.1% V5 (many diffs) n/a
19 TRCN0000479809 GGTCGAATGGTCGCATTAGATTCC pLX_317 80.6% 11.1% V5 (many diffs) n/a
20 ccsbBroadEn_15631 pDONR223 0% 11% None (many diffs) n/a
21 ccsbBroad304_15631 pLX_304 0% 11% V5 (many diffs) n/a
22 ccsbBroadEn_01892 pDONR223 100% 11.1% None (many diffs) n/a
23 ccsbBroad304_01892 pLX_304 0% 11.1% V5 (many diffs) n/a
24 TRCN0000473552 GACAAACTTCCATCCTCTTGAACC pLX_317 98.5% 11.1% V5 (many diffs) n/a
25 ccsbBroadEn_11266 pDONR223 100% 11% None (many diffs) n/a
26 ccsbBroad304_11266 pLX_304 0% 11% V5 (many diffs) n/a
27 TRCN0000473665 CTTGAACTGGACTCCCACCGCACC pLX_317 98.5% 11% V5 (many diffs) n/a
28 ccsbBroadEn_01895 pDONR223 100% 11% None (many diffs) n/a
29 ccsbBroad304_01895 pLX_304 0% 11% V5 (many diffs) n/a
30 TRCN0000468567 AACCATAGCTAACCCTGCACACAC pLX_317 100% 11% V5 (many diffs) n/a
31 ccsbBroadEn_07222 pDONR223 100% 10.9% None (many diffs) n/a
32 ccsbBroad304_07222 pLX_304 0% 10.9% V5 (many diffs) n/a
33 TRCN0000474296 CCGAGAACTTAGACACGTTCATCC pLX_317 98.5% 10.9% V5 (many diffs) n/a
34 ccsbBroadEn_15628 pDONR223 0% 10.9% None (many diffs) n/a
35 ccsbBroad304_15628 pLX_304 0% 10.9% V5 (many diffs) n/a
36 TRCN0000474646 TCTACAGTCTCGTAGCTATGTTCA pLX_317 100% 10.9% V5 (many diffs) n/a
37 ccsbBroadEn_01894 pDONR223 100% 10.9% None (many diffs) n/a
38 ccsbBroad304_01894 pLX_304 0% 10.9% V5 (many diffs) n/a
39 TRCN0000466135 ATCGATAACCATCGCTTACCTGGA pLX_317 72.6% 10.9% V5 (many diffs) n/a
40 ccsbBroadEn_01896 pDONR223 100% 10.9% None (many diffs) n/a
41 ccsbBroad304_01896 pLX_304 0% 10.9% V5 (many diffs) n/a
42 TRCN0000480994 TCCGAGTATGCGTATGATAAGCCC pLX_317 94.3% 10.9% V5 (many diffs) n/a
43 ccsbBroadEn_15629 pDONR223 0% 10.8% None (many diffs) n/a
44 ccsbBroad304_15629 pLX_304 0% 10.8% V5 (many diffs) n/a
45 TRCN0000469353 AGACAGTAAACCCGGGATGCGCAC pLX_317 73.4% 10.8% V5 (many diffs) n/a
46 ccsbBroadEn_03650 pDONR223 100% 10.7% None (many diffs) n/a
47 ccsbBroad304_03650 pLX_304 0% 10.7% V5 (many diffs) n/a
48 TRCN0000478286 ACAGCCCAAGTATGTTGCTGTGAT pLX_317 83.7% 10.7% V5 (many diffs) n/a
49 ccsbBroadEn_09253 pDONR223 100% 10.7% None (many diffs) n/a
50 ccsbBroad304_09253 pLX_304 0% 10.7% V5 (many diffs) n/a
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