Transcript: Mouse NM_178186.3

Mus musculus histone cluster 1, H2ag (Hist1h2ag), mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Hist1h2ag (319167)
Length:
472
CDS:
23..415

Additional Resources:

NCBI RefSeq record:
NM_178186.3
NBCI Gene record:
Hist1h2ag (319167)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_178186.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000074588 CAACAAGAAGACGCGCATCAT pLKO.1 241 CDS 100% 4.950 2.475 Y H2AC18 n/a
2 TRCN0000242129 CAACAAGAAGACGCGCATCAT pLKO_005 241 CDS 100% 4.950 2.475 Y Hist1h2ad n/a
3 TRCN0000242133 CAACGACGAGGAGCTCAACAA pLKO_005 289 CDS 100% 4.950 2.475 Y Hist1h2ag n/a
4 TRCN0000364413 CAACGACGAGGAGCTCAACAA pLKO_005 289 CDS 100% 4.950 2.475 Y H2AX n/a
5 TRCN0000097001 ACAACAAGAAGACGCGCATCA pLKO.1 240 CDS 100% 4.050 2.025 Y Hist2h2ac n/a
6 TRCN0000256521 ACAACAAGAAGACGCGCATCA pLKO_005 240 CDS 100% 4.050 2.025 Y H2AC19 n/a
7 TRCN0000257783 ACAAGAAGACGCGCATCATCC pLKO_005 243 CDS 100% 4.050 2.025 Y Hist1h2an n/a
8 TRCN0000429705 ACAAGAAGACGCGCATCATCC pLKO_005 243 CDS 100% 4.050 2.025 Y H2AC20 n/a
9 TRCN0000257997 GACAACAAGAAGACGCGCATC pLKO_005 239 CDS 100% 2.250 1.125 Y Hist1h2af n/a
10 TRCN0000092689 CGACAACAAGAAGACGCGCAT pLKO.1 238 CDS 100% 2.160 1.080 Y Hist2h2aa2 n/a
11 TRCN0000429526 CGACAACAAGAAGACGCGCAT pLKO_005 238 CDS 100% 2.160 1.080 Y H2AW n/a
12 TRCN0000369835 GCAACGACGAGGAGCTCAACA pLKO_005 288 CDS 100% 1.650 0.825 Y H2AC17 n/a
13 TRCN0000097017 TGCTCCGCAAGGGCAACTACT pLKO.1 123 CDS 100% 1.650 0.825 Y Hist1h2ap n/a
14 TRCN0000097020 GAAGACCGAGAGCCACCACAA pLKO.1 379 CDS 100% 1.350 0.675 Y Hist1h2ae n/a
15 TRCN0000433810 CGACGAGGAGCTCAACAAGCT pLKO_005 292 CDS 100% 0.880 0.440 Y H2AW n/a
16 TRCN0000255257 CAAGAAGACGCGCATCATCCC pLKO_005 244 CDS 100% 0.720 0.360 Y Hist2h2ab n/a
17 TRCN0000097023 CGAGGAGCTCAACAAGCTGCT pLKO.1 295 CDS 100% 0.720 0.360 Y Hist1h2ae n/a
18 TRCN0000092692 GCGACAACAAGAAGACGCGCA pLKO.1 237 CDS 100% 0.180 0.090 Y Hist2h2aa2 n/a
19 TRCN0000258002 ACCTGACGGCCGAGATCCTGG pLKO_005 195 CDS 100% 0.000 0.000 Y Hist1h2af n/a
20 TRCN0000256452 ACGGCCGAGATCCTGGAGCTG pLKO_005 200 CDS 100% 0.000 0.000 Y Hist1h2ai n/a
21 TRCN0000256979 AGGCTCGCGCCAAGGCCAAGA pLKO_005 51 CDS 100% 0.000 0.000 Y Hist1h2ad n/a
22 TRCN0000242134 CAACATCCAGGCCGTGCTGCT pLKO_005 352 CDS 100% 0.000 0.000 Y Hist1h2ag n/a
23 TRCN0000257802 CAAGGCCAAGACCCGCTCCTC pLKO_005 61 CDS 100% 0.000 0.000 Y Hist1h2an n/a
24 TRCN0000255948 CAAGGCTCGCGCCAAGGCCAA pLKO_005 49 CDS 100% 0.000 0.000 Y Hist1h2ah n/a
25 TRCN0000097015 CCAAGGCCAAGACCCGCTCCT pLKO.1 60 CDS 100% 0.000 0.000 Y Hist1h2ap n/a
26 TRCN0000255946 CCCGCGACAACAAGAAGACGC pLKO_005 234 CDS 100% 0.000 0.000 Y Hist1h2ah n/a
27 TRCN0000097030 CCGCAACGACGAGGAGCTCAA pLKO.1 286 CDS 100% 0.000 0.000 Y Hist1h2ab n/a
28 TRCN0000097018 CCGCAAGGGCAACTACTCGGA pLKO.1 127 CDS 100% 0.000 0.000 Y Hist1h2ap n/a
29 TRCN0000255950 CCTGACGGCCGAGATCCTGGA pLKO_005 196 CDS 100% 0.000 0.000 Y Hist1h2ah n/a
30 TRCN0000097032 CCTGCAGCTGGCCATCCGCAA pLKO.1 271 CDS 100% 0.000 0.000 Y Hist1h2ab n/a
31 TRCN0000073282 CCTGCCCAACATCCAGGCCGT pLKO.1 346 CDS 100% 0.000 0.000 Y H2AX n/a
32 TRCN0000097028 CGAGAGCCACCACAAGGCCAA pLKO.1 385 CDS 100% 0.000 0.000 Y Hist1h2ac n/a
33 TRCN0000257770 CGGCCGTGCTGGAGTACCTGA pLKO_005 180 CDS 100% 0.000 0.000 Y Hist1h2an n/a
34 TRCN0000250198 CTCCGCAAGGGCAACTACTCG pLKO_005 125 CDS 100% 0.000 0.000 Y Hist1h2af n/a
35 TRCN0000257792 CTGCTCCGCAAGGGCAACTAC pLKO_005 122 CDS 100% 0.000 0.000 Y Hist1h2an n/a
36 TRCN0000369944 CTGCTCCGCAAGGGCAACTAC pLKO_005 122 CDS 100% 0.000 0.000 Y H2AC17 n/a
37 TRCN0000242130 GCAACTACTCGGAGCGCGTGG pLKO_005 135 CDS 100% 0.000 0.000 Y Hist1h2ad n/a
38 TRCN0000242131 GCAGCTGGCCATCCGCAACGA pLKO_005 274 CDS 100% 0.000 0.000 Y Hist1h2ag n/a
39 TRCN0000097022 GCCCAAGAAGACCGAGAGCCA pLKO.1 373 CDS 100% 0.000 0.000 Y Hist1h2ae n/a
40 TRCN0000255949 GCCGTGCTGGAGTACCTGACG pLKO_005 182 CDS 100% 0.000 0.000 Y Hist1h2ah n/a
41 TRCN0000256987 GCTCCGCAAGGGCAACTACTC pLKO_005 124 CDS 100% 0.000 0.000 Y Hist1h2ad n/a
42 TRCN0000097014 GCTGCTCCGCAAGGGCAACTA pLKO.1 121 CDS 100% 0.000 0.000 Y Hist1h2ap n/a
43 TRCN0000097016 GCTGGAGTACCTGACGGCCGA pLKO.1 187 CDS 100% 0.000 0.000 Y Hist1h2ap n/a
44 TRCN0000097031 GCTGGCCATCCGCAACGACGA pLKO.1 277 CDS 100% 0.000 0.000 Y Hist1h2ab n/a
45 TRCN0000242128 GGAGTACCTGACGGCCGAGAT pLKO_005 190 CDS 100% 0.000 0.000 Y Hist1h2ad n/a
46 TRCN0000242136 GGCAACTACTCGGAGCGCGTG pLKO_005 134 CDS 100% 0.000 0.000 Y Hist1h2ak n/a
47 TRCN0000250199 GGCCGTGCTGGAGTACCTGAC pLKO_005 181 CDS 100% 0.000 0.000 Y Hist1h2af n/a
48 TRCN0000265807 GGCTGCTCCGCAAGGGCAACT pLKO_005 120 CDS 100% 0.000 0.000 Y Hist1h2ai n/a
49 TRCN0000256453 GGGCAACTACTCGGAGCGCGT pLKO_005 133 CDS 100% 0.000 0.000 Y Hist1h2ai n/a
50 TRCN0000257780 TACCTGACGGCCGAGATCCTG pLKO_005 194 CDS 100% 0.000 0.000 Y Hist1h2an n/a
51 TRCN0000255947 TCCGCAAGGGCAACTACTCGG pLKO_005 126 CDS 100% 0.000 0.000 Y Hist1h2ah n/a
52 TRCN0000256451 TGGAGTACCTGACGGCCGAGA pLKO_005 189 CDS 100% 0.000 0.000 Y Hist1h2ai n/a
53 TRCN0000106912 TGCTCCGCAAGGGCAACTATT pLKO.1 123 CDS 100% 13.200 6.600 Y H2AW n/a
54 TRCN0000106732 CTGCTCCGCAAGGGCAACTAT pLKO.1 122 CDS 100% 1.875 0.938 Y H2AC16 n/a
55 TRCN0000368904 TGCTGCTGCCCAAGAAGACCA pLKO_005 366 CDS 100% 0.880 0.440 Y H2AX n/a
56 TRCN0000106659 CCAAGGCCAAGACCCGCTCTT pLKO.1 60 CDS 100% 0.000 0.000 Y H2AC14 n/a
57 TRCN0000256450 CTCGCGCCAAGGCCAAGACTC pLKO_005 54 CDS 100% 0.000 0.000 Y Hist1h2ai n/a
58 TRCN0000074592 GCCATCCGCAACGACGAGGAA pLKO.1 281 CDS 100% 0.000 0.000 Y H2AC18 n/a
59 TRCN0000106666 GCCGAGATCCTGGAGCTGGCT pLKO.1 203 CDS 100% 0.000 0.000 Y H2AC13 n/a
60 TRCN0000096998 GCTCAACAAGCTGCTGGGCAA pLKO.1 301 CDS 100% 0.720 0.360 Y Hist2h2aa1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178186.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04591 pDONR223 100% 92% 99.2% None (many diffs) n/a
2 ccsbBroad304_04591 pLX_304 0% 92% 99.2% V5 (many diffs) n/a
3 TRCN0000472223 CCTTGCTACGATGTATGAGACTTA pLX_317 100% 92% 99.2% V5 (many diffs) n/a
4 ccsbBroadEn_01893 pDONR223 100% 90.2% 98.4% None (many diffs) n/a
5 ccsbBroad304_01893 pLX_304 97.8% 90.2% 98.4% V5 (many diffs) n/a
6 TRCN0000469277 CTTGTACACGCATGGACCTTCTTT pLX_317 100% 90.2% 98.4% V5 (many diffs) n/a
7 ccsbBroadEn_01891 pDONR223 100% 89.2% 98.4% None (many diffs) n/a
8 ccsbBroad304_01891 pLX_304 0% 89.2% 98.4% V5 (many diffs) n/a
9 TRCN0000481190 CGTGTTGGCCGTTCGATTTAGAAT pLX_317 98.5% 89.2% 98.4% V5 (many diffs) n/a
10 ccsbBroadEn_01892 pDONR223 100% 88.4% 98.4% None (many diffs) n/a
11 ccsbBroad304_01892 pLX_304 0% 88.4% 98.4% V5 (many diffs) n/a
12 TRCN0000473552 GACAAACTTCCATCCTCTTGAACC pLX_317 98.5% 88.4% 98.4% V5 (many diffs) n/a
13 ccsbBroadEn_02055 pDONR223 100% 88.2% 98.4% None (many diffs) n/a
14 ccsbBroad304_02055 pLX_304 0% 88.2% 98.4% V5 (many diffs) n/a
15 TRCN0000466333 TGGGGTTTAATTGCATTCTTGTTA pLX_317 72.6% 88.2% 98.4% V5 (many diffs) n/a
16 ccsbBroadEn_01895 pDONR223 100% 87.9% 98.4% None (many diffs) n/a
17 ccsbBroad304_01895 pLX_304 0% 87.9% 98.4% V5 (many diffs) n/a
18 TRCN0000468567 AACCATAGCTAACCCTGCACACAC pLX_317 100% 87.9% 98.4% V5 (many diffs) n/a
19 ccsbBroadEn_01894 pDONR223 100% 87.4% 98.4% None (many diffs) n/a
20 ccsbBroad304_01894 pLX_304 0% 87.4% 98.4% V5 (many diffs) n/a
21 TRCN0000466135 ATCGATAACCATCGCTTACCTGGA pLX_317 72.6% 87.4% 98.4% V5 (many diffs) n/a
22 ccsbBroadEn_15628 pDONR223 0% 87.1% 96.1% None (many diffs) n/a
23 ccsbBroad304_15628 pLX_304 0% 87.1% 96.1% V5 (many diffs) n/a
24 TRCN0000474646 TCTACAGTCTCGTAGCTATGTTCA pLX_317 100% 87.1% 96.1% V5 (many diffs) n/a
25 ccsbBroadEn_07222 pDONR223 100% 87.1% 96.1% None (many diffs) n/a
26 ccsbBroad304_07222 pLX_304 0% 87.1% 96.1% V5 (many diffs) n/a
27 TRCN0000474296 CCGAGAACTTAGACACGTTCATCC pLX_317 98.5% 87.1% 96.1% V5 (many diffs) n/a
28 ccsbBroadEn_15629 pDONR223 0% 87.1% 98.4% None (many diffs) n/a
29 ccsbBroad304_15629 pLX_304 0% 87.1% 98.4% V5 (many diffs) n/a
30 TRCN0000469353 AGACAGTAAACCCGGGATGCGCAC pLX_317 73.4% 87.1% 98.4% V5 (many diffs) n/a
31 ccsbBroadEn_09253 pDONR223 100% 86.4% 96.9% None (many diffs) n/a
32 ccsbBroad304_09253 pLX_304 0% 86.4% 96.9% V5 (many diffs) n/a
33 TRCN0000479809 GGTCGAATGGTCGCATTAGATTCC pLX_317 80.6% 85.8% 96.9% V5 (many diffs) n/a
34 ccsbBroadEn_15631 pDONR223 0% 85.6% 96.9% None (many diffs) n/a
35 ccsbBroad304_15631 pLX_304 0% 85.6% 96.9% V5 (many diffs) n/a
36 ccsbBroadEn_15630 pDONR223 0% 85.8% 96.9% None (many diffs) n/a
37 ccsbBroad304_15630 pLX_304 0% 85.8% 96.9% V5 (many diffs) n/a
38 TRCN0000466536 CGATTGTCATTCGGCCGACTATTC pLX_317 51.9% 85.8% 96.9% V5 (many diffs) n/a
39 ccsbBroadEn_03650 pDONR223 100% 85.8% 93.8% None (many diffs) n/a
40 ccsbBroad304_03650 pLX_304 0% 85.8% 93.8% V5 (many diffs) n/a
41 TRCN0000478286 ACAGCCCAAGTATGTTGCTGTGAT pLX_317 83.7% 85.8% 93.8% V5 (many diffs) n/a
42 ccsbBroadEn_11266 pDONR223 100% 85.6% 96.9% None (many diffs) n/a
43 ccsbBroad304_11266 pLX_304 0% 85.6% 96.9% V5 (many diffs) n/a
44 TRCN0000473665 CTTGAACTGGACTCCCACCGCACC pLX_317 98.5% 85.6% 96.9% V5 (many diffs) n/a
45 ccsbBroadEn_01896 pDONR223 100% 85.3% 95.3% None (many diffs) n/a
46 ccsbBroad304_01896 pLX_304 0% 85.3% 95.3% V5 (many diffs) n/a
47 TRCN0000480994 TCCGAGTATGCGTATGATAAGCCC pLX_317 94.3% 85.3% 95.3% V5 (many diffs) n/a
48 ccsbBroadEn_00717 pDONR223 100% 80.9% 83.2% None (many diffs) n/a
49 ccsbBroad304_00717 pLX_304 0% 80.9% 83.2% V5 (many diffs) n/a
50 TRCN0000469289 TCCAAGCCCTCATTGGAGCTGGTC pLX_317 100% 80.9% 83.2% V5 (many diffs) n/a
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