Transcript: Human NM_080596.2

Homo sapiens H2A clustered histone 12 (H2AC12), mRNA.

Source:
NCBI, updated 2019-09-25
Taxon:
Homo sapiens (human)
Gene:
H2AC12 (85235)
Length:
481
CDS:
48..434

Additional Resources:

NCBI RefSeq record:
NM_080596.2
NBCI Gene record:
H2AC12 (85235)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_080596.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000106761 CGCAAGGGTAATTATGCCGAG pLKO.1 153 CDS 100% 1.200 1.680 N H2AC12 n/a
2 TRCN0000106762 CTGCTCCGCAAGGGTAATTAT pLKO.1 147 CDS 100% 15.000 10.500 N H2AC12 n/a
3 TRCN0000436805 GGTGGTGTCTTGCCCAATATC pLKO_005 363 CDS 100% 13.200 7.920 N H2AC12 n/a
4 TRCN0000106763 CAACAAGAAGACCCGTATCAT pLKO.1 266 CDS 100% 5.625 3.375 N H2AC12 n/a
5 TRCN0000432920 AGACTGAGAGCCACCATAAGG pLKO_005 406 CDS 100% 4.950 2.970 N H2AC12 n/a
6 TRCN0000106760 CGACAACAAGAAGACCCGTAT pLKO.1 263 CDS 100% 4.050 2.430 N H2AC12 n/a
7 TRCN0000435499 GGAGTACCTGACCGCTGAGAT pLKO_005 215 CDS 100% 1.650 0.990 N H2AC12 n/a
8 TRCN0000242133 CAACGACGAGGAGCTCAACAA pLKO_005 314 CDS 100% 4.950 2.475 Y Hist1h2ag n/a
9 TRCN0000364413 CAACGACGAGGAGCTCAACAA pLKO_005 314 CDS 100% 4.950 2.475 Y H2AX n/a
10 TRCN0000096995 CTCAACAAGCTGCTGGGCAAA pLKO.1 327 CDS 100% 4.050 2.025 Y Hist2h2aa1 n/a
11 TRCN0000369835 GCAACGACGAGGAGCTCAACA pLKO_005 313 CDS 100% 1.650 0.825 Y H2AC17 n/a
12 TRCN0000433810 CGACGAGGAGCTCAACAAGCT pLKO_005 317 CDS 100% 0.880 0.440 Y H2AW n/a
13 TRCN0000106764 GCTGCTGGGCAAAGTCACCAT pLKO.1 335 CDS 100% 0.880 0.440 Y H2AC12 n/a
14 TRCN0000097023 CGAGGAGCTCAACAAGCTGCT pLKO.1 320 CDS 100% 0.720 0.360 Y Hist1h2ae n/a
15 TRCN0000096998 GCTCAACAAGCTGCTGGGCAA pLKO.1 326 CDS 100% 0.720 0.360 Y Hist2h2aa1 n/a
16 TRCN0000106659 CCAAGGCCAAGACCCGCTCTT pLKO.1 85 CDS 100% 0.000 0.000 Y H2AC14 n/a
17 TRCN0000097030 CCGCAACGACGAGGAGCTCAA pLKO.1 311 CDS 100% 0.000 0.000 Y Hist1h2ab n/a
18 TRCN0000106667 CTGGGCAAAGTCACCATCGCA pLKO.1 339 CDS 100% 0.250 0.125 Y H2AC13 n/a
19 TRCN0000097015 CCAAGGCCAAGACCCGCTCCT pLKO.1 85 CDS 100% 0.000 0.000 Y Hist1h2ap n/a
20 TRCN0000255946 CCCGCGACAACAAGAAGACGC pLKO_005 259 CDS 100% 0.000 0.000 Y Hist1h2ah n/a
21 TRCN0000256450 CTCGCGCCAAGGCCAAGACTC pLKO_005 79 CDS 100% 0.000 0.000 Y Hist1h2ai n/a
22 TRCN0000074592 GCCATCCGCAACGACGAGGAA pLKO.1 306 CDS 100% 0.000 0.000 Y H2AC18 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_080596.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09253 pDONR223 100% 99.2% 100% None 9A>G;15C>T;21A>G n/a
2 ccsbBroad304_09253 pLX_304 0% 99.2% 100% V5 9A>G;15C>T;21A>G n/a
3 ccsbBroadEn_15628 pDONR223 0% 90.6% 99.2% None (many diffs) n/a
4 ccsbBroad304_15628 pLX_304 0% 90.6% 99.2% V5 (many diffs) n/a
5 TRCN0000474646 TCTACAGTCTCGTAGCTATGTTCA pLX_317 100% 90.6% 99.2% V5 (many diffs) n/a
6 ccsbBroadEn_07222 pDONR223 100% 90.3% 99.2% None (many diffs) n/a
7 ccsbBroad304_07222 pLX_304 0% 90.3% 99.2% V5 (many diffs) n/a
8 TRCN0000474296 CCGAGAACTTAGACACGTTCATCC pLX_317 98.5% 90.3% 99.2% V5 (many diffs) n/a
9 ccsbBroadEn_02055 pDONR223 100% 89.4% 98.4% None (many diffs) n/a
10 ccsbBroad304_02055 pLX_304 0% 89.4% 98.4% V5 (many diffs) n/a
11 TRCN0000466333 TGGGGTTTAATTGCATTCTTGTTA pLX_317 72.6% 89.4% 98.4% V5 (many diffs) n/a
12 ccsbBroadEn_01891 pDONR223 100% 89.4% 98.4% None (many diffs) n/a
13 ccsbBroad304_01891 pLX_304 0% 89.4% 98.4% V5 (many diffs) n/a
14 TRCN0000481190 CGTGTTGGCCGTTCGATTTAGAAT pLX_317 98.5% 89.4% 98.4% V5 (many diffs) n/a
15 ccsbBroadEn_01892 pDONR223 100% 88.7% 98.4% None (many diffs) n/a
16 ccsbBroad304_01892 pLX_304 0% 88.7% 98.4% V5 (many diffs) n/a
17 TRCN0000473552 GACAAACTTCCATCCTCTTGAACC pLX_317 98.5% 88.7% 98.4% V5 (many diffs) n/a
18 ccsbBroadEn_01893 pDONR223 100% 88.4% 98.4% None (many diffs) n/a
19 ccsbBroad304_01893 pLX_304 97.8% 88.4% 98.4% V5 (many diffs) n/a
20 TRCN0000469277 CTTGTACACGCATGGACCTTCTTT pLX_317 100% 88.4% 98.4% V5 (many diffs) n/a
21 ccsbBroadEn_01895 pDONR223 100% 86.1% 98.4% None (many diffs) n/a
22 ccsbBroad304_01895 pLX_304 0% 86.1% 98.4% V5 (many diffs) n/a
23 TRCN0000468567 AACCATAGCTAACCCTGCACACAC pLX_317 100% 86.1% 98.4% V5 (many diffs) n/a
24 ccsbBroadEn_01894 pDONR223 100% 85.1% 96.9% None (many diffs) n/a
25 ccsbBroad304_01894 pLX_304 0% 85.1% 96.9% V5 (many diffs) n/a
26 TRCN0000466135 ATCGATAACCATCGCTTACCTGGA pLX_317 72.6% 85.1% 96.9% V5 (many diffs) n/a
27 ccsbBroadEn_15629 pDONR223 0% 84.8% 96.9% None (many diffs) n/a
28 ccsbBroad304_15629 pLX_304 0% 84.8% 96.9% V5 (many diffs) n/a
29 TRCN0000469353 AGACAGTAAACCCGGGATGCGCAC pLX_317 73.4% 84.8% 96.9% V5 (many diffs) n/a
30 ccsbBroadEn_15630 pDONR223 0% 84.6% 96.9% None (many diffs) n/a
31 ccsbBroad304_15630 pLX_304 0% 84.6% 96.9% V5 (many diffs) n/a
32 TRCN0000466536 CGATTGTCATTCGGCCGACTATTC pLX_317 51.9% 84.6% 96.9% V5 (many diffs) n/a
33 ccsbBroadEn_15631 pDONR223 0% 84.3% 96.9% None (many diffs) n/a
34 ccsbBroad304_15631 pLX_304 0% 84.3% 96.9% V5 (many diffs) n/a
35 TRCN0000479809 GGTCGAATGGTCGCATTAGATTCC pLX_317 80.6% 84.1% 96.9% V5 (many diffs) n/a
36 ccsbBroadEn_11266 pDONR223 100% 84.3% 96.9% None (many diffs) n/a
37 ccsbBroad304_11266 pLX_304 0% 84.3% 96.9% V5 (many diffs) n/a
38 TRCN0000473665 CTTGAACTGGACTCCCACCGCACC pLX_317 98.5% 84.3% 96.9% V5 (many diffs) n/a
39 ccsbBroadEn_04591 pDONR223 100% 84.1% 96.1% None (many diffs) n/a
40 ccsbBroad304_04591 pLX_304 0% 84.1% 96.1% V5 (many diffs) n/a
41 TRCN0000472223 CCTTGCTACGATGTATGAGACTTA pLX_317 100% 84.1% 96.1% V5 (many diffs) n/a
42 ccsbBroadEn_01896 pDONR223 100% 83.3% 96.1% None (many diffs) n/a
43 ccsbBroad304_01896 pLX_304 0% 83.3% 96.1% V5 (many diffs) n/a
44 TRCN0000480994 TCCGAGTATGCGTATGATAAGCCC pLX_317 94.3% 83.3% 96.1% V5 (many diffs) n/a
45 ccsbBroadEn_03650 pDONR223 100% 81.9% 95.3% None (many diffs) n/a
46 ccsbBroad304_03650 pLX_304 0% 81.9% 95.3% V5 (many diffs) n/a
47 TRCN0000478286 ACAGCCCAAGTATGTTGCTGTGAT pLX_317 83.7% 81.9% 95.3% V5 (many diffs) n/a
48 ccsbBroadEn_00717 pDONR223 100% 76% 82.5% None (many diffs) n/a
49 ccsbBroad304_00717 pLX_304 0% 76% 82.5% V5 (many diffs) n/a
50 TRCN0000469289 TCCAAGCCCTCATTGGAGCTGGTC pLX_317 100% 76% 82.5% V5 (many diffs) n/a
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