Transcript: Human NM_033445.2

Homo sapiens H2A.W histone (H2AW), mRNA.

Source:
NCBI, updated 2019-08-06
Taxon:
Homo sapiens (human)
Gene:
H2AW (92815)
Length:
496
CDS:
43..435

Additional Resources:

NCBI RefSeq record:
NM_033445.2
NBCI Gene record:
H2AW (92815)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_033445.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000106911 CGAGTACTTGACTGCCGAGAT pLKO.1 210 CDS 100% 4.050 2.835 N H2AW n/a
2 TRCN0000106910 CCGCGGTGCTCGAGTACTTGA pLKO.1 200 CDS 100% 0.000 0.000 N H2AW n/a
3 TRCN0000092689 CGACAACAAGAAGACGCGCAT pLKO.1 258 CDS 100% 2.160 1.296 N Hist2h2aa2 n/a
4 TRCN0000429526 CGACAACAAGAAGACGCGCAT pLKO_005 258 CDS 100% 2.160 1.296 N H2AW n/a
5 TRCN0000106914 CCAAGGCTAAGTCGCGCTCGT pLKO.1 80 CDS 100% 0.000 0.000 N H2AW n/a
6 TRCN0000096997 GCCCAAGAAGACGGAGAGCCA pLKO.1 393 CDS 100% 0.000 0.000 N Hist2h2aa1 n/a
7 TRCN0000106912 TGCTCCGCAAGGGCAACTATT pLKO.1 143 CDS 100% 13.200 6.600 Y H2AW n/a
8 TRCN0000074588 CAACAAGAAGACGCGCATCAT pLKO.1 261 CDS 100% 4.950 2.475 Y H2AC18 n/a
9 TRCN0000242129 CAACAAGAAGACGCGCATCAT pLKO_005 261 CDS 100% 4.950 2.475 Y Hist1h2ad n/a
10 TRCN0000242133 CAACGACGAGGAGCTCAACAA pLKO_005 309 CDS 100% 4.950 2.475 Y Hist1h2ag n/a
11 TRCN0000364413 CAACGACGAGGAGCTCAACAA pLKO_005 309 CDS 100% 4.950 2.475 Y H2AX n/a
12 TRCN0000097001 ACAACAAGAAGACGCGCATCA pLKO.1 260 CDS 100% 4.050 2.025 Y Hist2h2ac n/a
13 TRCN0000256521 ACAACAAGAAGACGCGCATCA pLKO_005 260 CDS 100% 4.050 2.025 Y H2AC19 n/a
14 TRCN0000257783 ACAAGAAGACGCGCATCATCC pLKO_005 263 CDS 100% 4.050 2.025 Y Hist1h2an n/a
15 TRCN0000429705 ACAAGAAGACGCGCATCATCC pLKO_005 263 CDS 100% 4.050 2.025 Y H2AC20 n/a
16 TRCN0000257997 GACAACAAGAAGACGCGCATC pLKO_005 259 CDS 100% 2.250 1.125 Y Hist1h2af n/a
17 TRCN0000369835 GCAACGACGAGGAGCTCAACA pLKO_005 308 CDS 100% 1.650 0.825 Y H2AC17 n/a
18 TRCN0000106913 ACTGCCGAGATCCTGGAGCTT pLKO.1 220 CDS 100% 0.880 0.440 Y H2AW n/a
19 TRCN0000433810 CGACGAGGAGCTCAACAAGCT pLKO_005 312 CDS 100% 0.880 0.440 Y H2AW n/a
20 TRCN0000255257 CAAGAAGACGCGCATCATCCC pLKO_005 264 CDS 100% 0.720 0.360 Y Hist2h2ab n/a
21 TRCN0000097023 CGAGGAGCTCAACAAGCTGCT pLKO.1 315 CDS 100% 0.720 0.360 Y Hist1h2ae n/a
22 TRCN0000092692 GCGACAACAAGAAGACGCGCA pLKO.1 257 CDS 100% 0.180 0.090 Y Hist2h2aa2 n/a
23 TRCN0000097030 CCGCAACGACGAGGAGCTCAA pLKO.1 306 CDS 100% 0.000 0.000 Y Hist1h2ab n/a
24 TRCN0000097032 CCTGCAGCTGGCCATCCGCAA pLKO.1 291 CDS 100% 0.000 0.000 Y Hist1h2ab n/a
25 TRCN0000073282 CCTGCCCAACATCCAGGCCGT pLKO.1 366 CDS 100% 0.000 0.000 Y H2AX n/a
26 TRCN0000242131 GCAGCTGGCCATCCGCAACGA pLKO_005 294 CDS 100% 0.000 0.000 Y Hist1h2ag n/a
27 TRCN0000097031 GCTGGCCATCCGCAACGACGA pLKO.1 297 CDS 100% 0.000 0.000 Y Hist1h2ab n/a
28 TRCN0000097017 TGCTCCGCAAGGGCAACTACT pLKO.1 143 CDS 100% 1.650 0.825 Y Hist1h2ap n/a
29 TRCN0000074592 GCCATCCGCAACGACGAGGAA pLKO.1 301 CDS 100% 0.000 0.000 Y H2AC18 n/a
30 TRCN0000096998 GCTCAACAAGCTGCTGGGCAA pLKO.1 321 CDS 100% 0.720 0.360 Y Hist2h2aa1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_033445.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04591 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04591 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000472223 CCTTGCTACGATGTATGAGACTTA pLX_317 100% 100% 100% V5 n/a
4 TRCN0000479809 GGTCGAATGGTCGCATTAGATTCC pLX_317 80.6% 86.9% 97.6% V5 (many diffs) n/a
5 ccsbBroadEn_15631 pDONR223 0% 86.6% 97.6% None (many diffs) n/a
6 ccsbBroad304_15631 pLX_304 0% 86.6% 97.6% V5 (many diffs) n/a
7 ccsbBroadEn_01893 pDONR223 100% 86.9% 97.6% None (many diffs) n/a
8 ccsbBroad304_01893 pLX_304 97.8% 86.9% 97.6% V5 (many diffs) n/a
9 TRCN0000469277 CTTGTACACGCATGGACCTTCTTT pLX_317 100% 86.9% 97.6% V5 (many diffs) n/a
10 ccsbBroadEn_01891 pDONR223 100% 86.9% 97.6% None (many diffs) n/a
11 ccsbBroad304_01891 pLX_304 0% 86.9% 97.6% V5 (many diffs) n/a
12 TRCN0000481190 CGTGTTGGCCGTTCGATTTAGAAT pLX_317 98.5% 86.9% 97.6% V5 (many diffs) n/a
13 ccsbBroadEn_15630 pDONR223 0% 86.6% 97.6% None (many diffs) n/a
14 ccsbBroad304_15630 pLX_304 0% 86.6% 97.6% V5 (many diffs) n/a
15 TRCN0000466536 CGATTGTCATTCGGCCGACTATTC pLX_317 51.9% 86.6% 97.6% V5 (many diffs) n/a
16 ccsbBroadEn_11266 pDONR223 100% 86.6% 97.6% None (many diffs) n/a
17 ccsbBroad304_11266 pLX_304 0% 86.6% 97.6% V5 (many diffs) n/a
18 TRCN0000473665 CTTGAACTGGACTCCCACCGCACC pLX_317 98.5% 86.6% 97.6% V5 (many diffs) n/a
19 ccsbBroadEn_01892 pDONR223 100% 86.4% 97.6% None (many diffs) n/a
20 ccsbBroad304_01892 pLX_304 0% 86.4% 97.6% V5 (many diffs) n/a
21 TRCN0000473552 GACAAACTTCCATCCTCTTGAACC pLX_317 98.5% 86.4% 97.6% V5 (many diffs) n/a
22 ccsbBroadEn_01894 pDONR223 100% 86.4% 99.2% None (many diffs) n/a
23 ccsbBroad304_01894 pLX_304 0% 86.4% 99.2% V5 (many diffs) n/a
24 TRCN0000466135 ATCGATAACCATCGCTTACCTGGA pLX_317 72.6% 86.4% 99.2% V5 (many diffs) n/a
25 ccsbBroadEn_02055 pDONR223 100% 86.1% 97.6% None (many diffs) n/a
26 ccsbBroad304_02055 pLX_304 0% 86.1% 97.6% V5 (many diffs) n/a
27 TRCN0000466333 TGGGGTTTAATTGCATTCTTGTTA pLX_317 72.6% 86.1% 97.6% V5 (many diffs) n/a
28 ccsbBroadEn_15629 pDONR223 0% 86.1% 99.2% None (many diffs) n/a
29 ccsbBroad304_15629 pLX_304 0% 86.1% 99.2% V5 (many diffs) n/a
30 TRCN0000469353 AGACAGTAAACCCGGGATGCGCAC pLX_317 73.4% 86.1% 99.2% V5 (many diffs) n/a
31 ccsbBroadEn_15628 pDONR223 0% 85.1% 95.3% None (many diffs) n/a
32 ccsbBroad304_15628 pLX_304 0% 85.1% 95.3% V5 (many diffs) n/a
33 TRCN0000474646 TCTACAGTCTCGTAGCTATGTTCA pLX_317 100% 85.1% 95.3% V5 (many diffs) n/a
34 ccsbBroadEn_07222 pDONR223 100% 85.1% 95.3% None (many diffs) n/a
35 ccsbBroad304_07222 pLX_304 0% 85.1% 95.3% V5 (many diffs) n/a
36 TRCN0000474296 CCGAGAACTTAGACACGTTCATCC pLX_317 98.5% 85.1% 95.3% V5 (many diffs) n/a
37 ccsbBroadEn_01895 pDONR223 100% 85.1% 97.6% None (many diffs) n/a
38 ccsbBroad304_01895 pLX_304 0% 85.1% 97.6% V5 (many diffs) n/a
39 TRCN0000468567 AACCATAGCTAACCCTGCACACAC pLX_317 100% 85.1% 97.6% V5 (many diffs) n/a
40 ccsbBroadEn_09253 pDONR223 100% 84.6% 96.1% None (many diffs) n/a
41 ccsbBroad304_09253 pLX_304 0% 84.6% 96.1% V5 (many diffs) n/a
42 ccsbBroadEn_01896 pDONR223 100% 84.1% 96.1% None (many diffs) n/a
43 ccsbBroad304_01896 pLX_304 0% 84.1% 96.1% V5 (many diffs) n/a
44 TRCN0000480994 TCCGAGTATGCGTATGATAAGCCC pLX_317 94.3% 84.1% 96.1% V5 (many diffs) n/a
45 ccsbBroadEn_03650 pDONR223 100% 83.5% 94.6% None (many diffs) n/a
46 ccsbBroad304_03650 pLX_304 0% 83.5% 94.6% V5 (many diffs) n/a
47 TRCN0000478286 ACAGCCCAAGTATGTTGCTGTGAT pLX_317 83.7% 83.5% 94.6% V5 (many diffs) n/a
48 ccsbBroadEn_00717 pDONR223 100% 78.3% 83.9% None (many diffs) n/a
49 ccsbBroad304_00717 pLX_304 0% 78.3% 83.9% V5 (many diffs) n/a
50 TRCN0000469289 TCCAAGCCCTCATTGGAGCTGGTC pLX_317 100% 78.3% 83.9% V5 (many diffs) n/a
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