Construct: ORF ccsbBroad304_02531
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013593.1_s304c1, BRDN0000399178
- Derived from:
- ccsbBroadEn_02531
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSPH1 (10808)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02531
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10808 | HSPH1 | heat shock protein family H... | NM_006644.4 | 100% | 100% | |
| 2 | human | 10808 | HSPH1 | heat shock protein family H... | NM_001349704.2 | 98.4% | 97.7% | (many diffs) |
| 3 | human | 10808 | HSPH1 | heat shock protein family H... | NM_001286504.1 | 97.5% | 95.9% | (many diffs) |
| 4 | human | 10808 | HSPH1 | heat shock protein family H... | XM_011534887.3 | 96% | 93.7% | (many diffs) |
| 5 | human | 10808 | HSPH1 | heat shock protein family H... | NM_001286503.1 | 94.8% | 94.8% | 1584_1585ins132 |
| 6 | human | 10808 | HSPH1 | heat shock protein family H... | XM_017020363.2 | 93.3% | 92.6% | (many diffs) |
| 7 | human | 10808 | HSPH1 | heat shock protein family H... | XM_005266236.2 | 92.4% | 90.8% | (many diffs) |
| 8 | human | 10808 | HSPH1 | heat shock protein family H... | XM_017020362.2 | 90.9% | 88.7% | (many diffs) |
| 9 | human | 10808 | HSPH1 | heat shock protein family H... | XM_011534888.2 | 90.9% | 90.9% | 429_430ins234 |
| 10 | human | 10808 | HSPH1 | heat shock protein family H... | NM_001286505.1 | 88.5% | 86.9% | (many diffs) |
| 11 | human | 10808 | HSPH1 | heat shock protein family H... | XM_017020364.2 | 85.7% | 85.7% | 429_430ins234;1350_1351ins132 |
| 12 | mouse | 15505 | Hsph1 | heat shock 105kDa/110kDa pr... | NM_013559.2 | 86.2% | 93.3% | (many diffs) |
| 13 | mouse | 15505 | Hsph1 | heat shock 105kDa/110kDa pr... | XM_006504916.2 | 82.9% | 87.7% | (many diffs) |
| 14 | mouse | 15505 | Hsph1 | heat shock 105kDa/110kDa pr... | NM_001347534.1 | 81.5% | 88.7% | (many diffs) |
| 15 | mouse | 15505 | Hsph1 | heat shock 105kDa/110kDa pr... | XM_006504915.3 | 76.4% | 82.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2640
- ORF length:
- 2574
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggtggtgggg ttggacgtgg gctcgcagag ctgctacatc gcggtagccc 121 gggccggggg catcgagacc atcgccaatg agttcagcga ccggtgcacc ccgtcagtca 181 tatcatttgg atcaaaaaat agaacaatcg gagttgcagc caaaaatcag caaatcactc 241 atgcaaacaa tacggtgtct aacttcaaaa gatttcatgg ccgagcattc aatgacccct 301 tcattcaaaa ggagaaggaa aacttgagtt acgatttggt tccattgaaa aatggtggag 361 ttggaataaa ggtaatgtac atgggtgaag aacatctatt tagtgtggag cagataacag 421 ccatgttgtt gactaagctg aaggaaactg ctgaaaacag cctcaagaaa ccagtaacag 481 attgtgttat ttcagtcccc tccttcttta cagatgctga gaggcgatct gtgttagatg 541 ctgcacagat tgttggccta aactgtttaa gacttatgaa tgacatgaca gctgttgctt 601 tgaattacgg aatttataag caggatctcc caagcctgga tgagaaacct cggatagtgg 661 tttttgttga tatgggacat tcagcttttc aagtgtctgc ttgtgctttt aacaagggaa 721 aattgaaggt actgggaaca gcttttgatc ctttcttagg aggaaaaaac ttcgatgaaa 781 agttagtgga acatttttgt gcagaattta aaactaagta caagttggat gcaaaatcca 841 aaatacgagc actcctacgt ctgtatcagg aatgtgaaaa actgaaaaag ctaatgagct 901 ctaacagcac agaccttcca ctgaatatcg aatgctttat gaatgataaa gatgtttccg 961 gaaagatgaa caggtcacaa tttgaagaac tctgtgctga acttctgcaa aagatagaag 1021 taccccttta ttcactgttg gaacaaactc atctcaaagt agaagatgtg agtgcagttg 1081 agattgttgg aggcgctaca cgaattccag ctgtgaagga aagaattgcc aaattctttg 1141 gaaaagatat tagcacaaca ctcaatgcag atgaagcagt agccagagga tgtgcattac 1201 agtgtgcaat actttccccg gcatttaaag ttagagaatt ttccgtcaca gatgcagttc 1261 cttttccaat atctctgatc tggaaccatg attcagaaga tactgaaggt gttcatgaag 1321 tctttagtcg aaaccatgct gctcctttct ccaaagttct cacctttctg agaagggggc 1381 cttttgagct agaagctttc tattctgatc cccaaggagt tccatatcca gaagcaaaaa 1441 taggccgctt tgtagttcag aatgtttctg cacagaaaga tggagaaaaa tctagagtaa 1501 aagtcaaagt gcgagtcaac acccatggca ttttcaccat ctctacggca tctatggtgg 1561 agaaagtccc aactgaggag aatgaaatgt cttctgaagc tgacatggag tgtctgaatc 1621 agagaccacc agaaaaccca gacactgata aaaatgtcca gcaagacaac agtgaagctg 1681 gaacacagcc ccaggtacaa actgatgctc aacaaacctc acagtctccc ccttcacctg 1741 aacttacctc agaagaaaac aaaatcccag atgctgacaa agcaaatgaa aaaaaagttg 1801 accagcctcc agaagctaaa aagcccaaaa taaaggtggt gaatgttgag ctgcctattg 1861 aagccaactt ggtctggcag ttagggaaag accttcttaa catgtatatt gagacagagg 1921 gtaagatgat aatgcaagat aaattggaaa aagaaaggaa tgatgctaaa aatgcagttg 1981 aggaatatgt gtatgagttc agagacaagc tgtgtggacc atatgaaaaa tttatatgtg 2041 agcaggatca tcaaaatttt ttgagactcc tcacagaaac tgaagactgg ctgtatgaag 2101 aaggagagga ccaagctaaa caagcatatg ttgacaagtt ggaagaatta atgaaaattg 2161 gcactccagt taaagttcgg tttcaggaag ctgaagaacg gccaaaaatg tttgaagaac 2221 taggacagag gctgcagcat tatgccaaga tagcagctga cttcagaaat aaggatgaga 2281 aatacaacca tattgatgag tctgaaatga aaaaagtgga gaagtctgtt aatgaagtga 2341 tggaatggat gaataatgtc atgaatgctc aggctaaaaa gagtcttgat caggatccag 2401 ttgtacgtgc tcaggaaatt aaaacaaaaa tcaaggaatt gaacaacaca tgtgaacccg 2461 ttgtaacaca accgaaacca aaaattgaat cacccaaact ggaaagaact ccaaatggcc 2521 caaatattga taaaaaggaa gaagatttag aagacaaaaa caattttggt gctgaacctc 2581 cacatcagaa tggtgaatgt taccctaatg agaaaaattc tgttaatatg gacttggact 2641 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 2701 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 2761 ggatta