Construct: ORF ccsbBroad304_03798
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010373.1_s304c1, BRDN0000401992
- Derived from:
- ccsbBroadEn_03798
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHTF2 (57157)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03798
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127357.2 | 100% | 100% | |
2 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_005250508.5 | 100% | 100% | |
3 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_005250509.4 | 100% | 100% | |
4 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127358.2 | 99.4% | 99.4% | 101_102insGTTTATTAAACT |
5 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366086.1 | 99.4% | 99.4% | 101_102insGTTTATTAAACT |
6 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_020432.5 | 99.4% | 99.4% | 101_102insGTTTATTAAACT |
7 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366089.1 | 95.6% | 95.6% | 45_146del |
8 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_006716063.4 | 94.2% | 94.2% | 1236_1237ins129 |
9 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_011516422.3 | 94.1% | 94.1% | 1636_1637ins132 |
10 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366084.1 | 93.6% | 93.6% | 101_102insGTTTATTAAACT;1624_1625ins132 |
11 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366087.1 | 93.6% | 93.6% | 101_102insGTTTATTAAACT;1624_1625ins132 |
12 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366081.1 | 92.5% | 92.5% | 101_102insGTTTATTAAACT;1842_1843ins156 |
13 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_011516423.3 | 90.2% | 90.2% | 1015_1016ins219 |
14 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_017012459.2 | 89.7% | 89.7% | 101_102insGTTTATTAAACT;1003_1004ins219 |
15 | human | 57157 | PHTF2 | putative homeodomain transc... | XM_011516424.2 | 81.2% | 81.2% | 0_1ins423 |
16 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366082.2 | 72.2% | 72.1% | (many diffs) |
17 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366088.2 | 72.2% | 72.1% | (many diffs) |
18 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366085.2 | 62.5% | 62.4% | (many diffs) |
19 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001366083.1 | 60.4% | 60.1% | (many diffs) |
20 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127359.1 | 46.7% | 44.9% | (many diffs) |
21 | human | 57157 | PHTF2 | putative homeodomain transc... | NM_001127360.1 | 46.2% | 44.4% | (many diffs) |
22 | mouse | 68770 | Phtf2 | putative homeodomain transc... | NM_172992.3 | 88.6% | 93.3% | (many diffs) |
23 | mouse | 68770 | Phtf2 | putative homeodomain transc... | XM_006535801.1 | 79.7% | 84.2% | (many diffs) |
24 | mouse | 68770 | Phtf2 | putative homeodomain transc... | XM_006535803.1 | 52.4% | 51% | (many diffs) |
25 | mouse | 68770 | Phtf2 | putative homeodomain transc... | XM_006535804.3 | 40.2% | 40% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2322
- ORF length:
- 2253
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgtccaaa gtcacagatg ctatagtctg gtatcaaaag aagattggag 121 catatgatca acaaatatgg gaaaaatctg ttgaacagag agaaatcaag tttattaaac 181 tggggctaag gaataaacca aagaaaacag cacatgtgaa accagacctc atagatgttg 241 atcttgtaag agggtctgca tttgcaaagg caaagcctga aagtccttgg acttctctga 301 ccagaaaggg aattgttcga gttgtatttt tccccttttt cttccggtgg tggttacaag 361 taacatcaaa ggtcatcttt ttctggcttc ttgtccttta tcttcttcaa gttgctgcaa 421 tagtattatt ctgctccact tctagcccac acagcatacc tctgacagag gtgattgggc 481 cgatatggct gatgctgctc ctgggaactg tgcattgcca gattgtttcc acaagaacac 541 ccaaacctcc tctaagtaca gggggtaaaa gaagaaggaa attaagaaaa gcagcccatt 601 tggaagtaca tagggaagga gatggttcta gtaccacaga taacacacaa gagggagcag 661 ttcagaacca cggtacaagc acctctcaca gcgttggcac tgtcttcaga gatctctggc 721 atgctgcttt ctttttatca ggatcaaaga aagcaaagaa ttcaattgat aaatcaactg 781 aaactgacaa tggctatgta tcccttgatg ggaagaagac tgttaaaagc ggtgaagatg 841 gaatacaaaa ccatgaacct cagtgtgaaa ctattcgacc agaagagaca gcctggaaca 901 caggaacact gaggaatggt cctagcaaag atacccaaag gacaataaca aatgtctctg 961 atgaagtctc cagtgaggaa ggtcctgaaa caggatactc attacgtcgt catgtggaca 1021 ggacttctga aggtgttctt cggaatagaa agtcacacca ttataagaaa cattacccta 1081 atgaggacgc ccctaaatcg ggtactagtt gcagctctcg ctgttcaagt tccagacagg 1141 attctgagag tgcaaggcca gaatctgaaa cagaagatgt gttatgggaa gacttgttac 1201 attgtgcaga atgccattca tcttgtacca gtgagacaga tgtggaaaat catcagatta 1261 atccatgtgt gaaaaaagaa tatagagatg acccttttca tcagagtcat ttgccctggc 1321 tccatagttc ccacccagga ttagaaaaaa taagtgctat agtatgggaa ggtaatgatt 1381 gtaagaaagc agacatgtct gtacttgaaa tcagtggaat gataatgaac agagtgaaca 1441 gccatatacc aggaatagga taccagattt ttggaaatgc agtctctctc atactgggtt 1501 taactccatt tgttttccga ctttctcaag ctacagactt ggaacaactc acagcacatt 1561 ctgcttcaga actttatgtg attgcatttg gttctaatga agatgtcata gttctttcta 1621 tggttataat aagttttgtg gttcgcgtgt ctcttgtgtg gattttcttt tttttgctct 1681 gtgtagcaga aagaacttat aaacagcgat tactttttgc aaaactcttt ggacatttaa 1741 catctgcaag gagggctcga aaatctgagg ttcctcattt ccggttgaag aaagtacaga 1801 atataaaaat gtggctatct ctccgttcct atcttaagcg tcgaggtcct cagcgatcag 1861 ttgatgtaat agtttcatct gctttcttat tgactatctc agttgtattt atctgttgtg 1921 cccagctact tcatgtacac gagatcttcc ttgattgtca ctacaattgg gaattggtaa 1981 tctggtgcat ctcgttaaca ctttttctcc taagatttgt tacccttgga tcagaaacaa 2041 gtaaaaaata tagtaatacc tcaatattac ttactgaaca gataaacctc tacttgaaaa 2101 tggagaaaaa acctaacaaa aaggaggaac tgacactagt gaataatgtt ttaaaactgg 2161 ctactaaact gctaaaggag ttggacagtc cttttagatt atatgggctt acaatgaatc 2221 cgctgcttta taacatcacc caggttgtta tcctgtcagc tgtttctggt gttatcagtg 2281 acttgcttgg atttaattta aagctatgga agattaagtc attgccaact ttcttgtaca 2341 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 2401 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta