Construct: ORF ccsbBroad304_03863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017020.1_s304c1, BRDN0000404136
- Derived from:
- ccsbBroadEn_03863
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JAM2 (58494)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03863
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58494 | JAM2 | junctional adhesion molecule 2 | NM_021219.4 | 100% | 100% | |
| 2 | human | 58494 | JAM2 | junctional adhesion molecule 2 | NM_001270408.2 | 94.1% | 89.7% | (many diffs) |
| 3 | human | 58494 | JAM2 | junctional adhesion molecule 2 | NM_001270407.1 | 87.9% | 87.9% | 129_130ins108 |
| 4 | human | 58494 | JAM2 | junctional adhesion molecule 2 | XM_024452123.1 | 70.4% | 70.4% | 0_1ins264 |
| 5 | human | 58494 | JAM2 | junctional adhesion molecule 2 | XM_024452124.1 | 70.4% | 70.4% | 0_1ins264 |
| 6 | human | 58494 | JAM2 | junctional adhesion molecule 2 | XR_937537.3 | 21.3% | 1_532del;1354_1555del;1629_4194del | |
| 7 | human | 58494 | JAM2 | junctional adhesion molecule 2 | NR_072999.2 | 20.4% | 1_564del;1257_1258insGTAG;1455_4347del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gaggaggagc cgccaccgcc tcctcctgct gctgctgcgc tacctggtgg 121 tcgccctggg ctatcataag gcctatgggt tttctgcccc aaaagaccaa caagtagtca 181 cagcagtaga gtaccaagag gctattttag cctgcaaaac cccaaagaag actgtttcct 241 ccagattaga gtggaagaaa ctgggtcgga gtgtctcctt tgtctactat caacagactc 301 ttcaaggtga ttttaaaaat cgagctgaga tgatagattt caatatccgg atcaaaaatg 361 tgacaagaag tgatgcgggg aaatatcgtt gtgaagttag tgccccatct gagcaaggcc 421 aaaacctgga agaggataca gtcactctgg aagtattagt ggctccagca gttccatcat 481 gtgaagtacc ctcttctgct ctgagtggaa ctgtggtaga gctacgatgt caagacaaag 541 aagggaatcc agctcctgaa tacacatggt ttaaggatgg catccgtttg ctagaaaatc 601 ccagacttgg ctcccaaagc accaacagct catacacaat gaatacaaaa actggaactc 661 tgcaatttaa tactgtttcc aaactggaca ctggagaata ttcctgtgaa gcccgcaatt 721 ctgttggata tcgcaggtgt cctgggaaac gaatgcaagt agatgatctc aacataagtg 781 gcatcatagc agccgtagta gttgtggcct tagtgatttc cgtttgtggc cttggtgtat 841 gctatgctca gaggaaaggc tacttttcaa aagaaacctc cttccagaag agtaattctt 901 catctaaagc cacgacaatg agtgaaaatg atttcaagca cacaaaatcc tttataattt 961 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1021 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1081 ggatta