Construct: ORF ccsbBroad304_05666

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003816.1_s304c1, BRDN0000401645
Derived from:
ccsbBroadEn_05666
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KRTAP5-6 (440023)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05666
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 440023 KRTAP5-6 keratin associated protein 5-6 NM_001012416.1 100% 100%
2 human 440050 KRTAP5-7 keratin associated protein 5-7 NM_001012503.2 72.7% 73.9% (many diffs)
3 human 440021 KRTAP5-2 keratin associated protein 5-2 NM_001004325.2 67.4% 66.1% (many diffs)
4 human 440051 KRTAP5-11 keratin associated protein ... NM_001005405.3 66.8% 66.2% (many diffs)
5 human 57830 KRTAP5-8 keratin associated protein 5-8 NM_021046.3 65.2% 65.7% (many diffs)
6 human 3846 KRTAP5-9 keratin associated protein 5-9 NM_005553.3 64.1% 60.5% (many diffs)
7 human 387273 KRTAP5-10 keratin associated protein ... NM_001012710.2 59.4% 59.9% (many diffs)
8 human 439915 KRTAP5-5 keratin associated protein 5-5 XM_006725534.2 53.3% 53.8% (many diffs)
9 human 387267 KRTAP5-4 keratin associated protein 5-4 NM_001347674.1 52% 54.8% (many diffs)
10 human 439915 KRTAP5-5 keratin associated protein 5-5 NM_001001480.2 50% 50.2% (many diffs)
11 human 387266 KRTAP5-3 keratin associated protein 5-3 NM_001012708.2 50% 50% (many diffs)
12 human 387264 KRTAP5-1 keratin associated protein 5-1 NM_001005922.1 42.3% 41.7% (many diffs)
13 human 387267 KRTAP5-4 keratin associated protein 5-4 NM_001012709.1 41.3% 43.4% (many diffs)
14 mouse 105244938 Gm40460 predicted gene, 40460 XM_017312478.1 46.4% 44.8% (many diffs)
15 mouse 114666 Krtap5-5 keratin associated protein 5-5 NM_001037822.1 46.1% 43.6% (many diffs)
16 mouse 100043617 Gm4553 predicted gene 4553 XM_030243137.1 37.5% 36.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
456
ORF length:
387
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggctgctgt ggctgctctg gaggctgtgg ctccggctgt gggggctgtg
121gctctggctg tgggggctgt gggtccagct gctgtgtgcc catctgctgc tgcaagcccg
181tgtgctgctg tgtgccagcc tgttcctgca ccagctgtgg ctcttgtggg ggctccaagg
241ggtgctgtgg ctcttgtggg ggctccaaag ggggctgtgg ctcttgtggg ggctccaagg
301gaggctgtgg ctcttgtggc tgctcccagt gcagttgctg caagccctgc tactgttcct
361caggctgtgg gtcatcctgc tgccagtcca gctgctgcaa gccctgctgt tcccaggcca
421gctgctgtgt ccccatttgc tgccagtgca aaatcttgcc aactttcttg tacaaagtgg
481ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
541ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)