Construct: ORF ccsbBroad304_06278

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002072.1_s304c1, BRDN0000400658
Derived from:
ccsbBroadEn_06278
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GK (2710)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06278
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2710 GK glycerol kinase NM_203391.3 99.8% 99.6% 237T>A;391C>A;501C>A
2 human 2710 GK glycerol kinase NM_000167.5 98.6% 98.4% (many diffs)
3 human 2710 GK glycerol kinase XM_011545492.1 94.8% 94.6% (many diffs)
4 human 2710 GK glycerol kinase NM_001205019.1 94.3% 94.2% (many diffs)
5 human 2710 GK glycerol kinase XM_006724484.2 93.7% 93.5% (many diffs)
6 human 2710 GK glycerol kinase NM_001128127.2 93.3% 93.2% (many diffs)
7 human 2710 GK glycerol kinase XM_011545491.2 89.8% 89.7% (many diffs)
8 human 2710 GK glycerol kinase XM_006724483.2 88.8% 88.7% (many diffs)
9 human 2712 GK2 glycerol kinase 2 NM_033214.3 82.5% 82.1% (many diffs)
10 human 2713 GK3P glycerol kinase 3 pseudogene NR_026575.1 68.1% (many diffs)
11 human 2710 GK glycerol kinase XM_017029410.1 61.3% 61.3% 0_1ins615
12 human 2710 GK glycerol kinase XM_017029411.1 60.1% 60.1% 0_1ins615;112_113insTGAAAATCTCTCATAGCG
13 human 2710 GK glycerol kinase XM_017029412.2 60.1% 60.1% 0_1ins615;112_113insTGAAAATCTCTCATAGCG
14 human 2710 GK glycerol kinase XM_006724485.2 57.9% 57.9% 0_1ins615;970_972delGACinsATT;975_1062delinsA
15 human 2710 GK glycerol kinase XM_006724486.3 57.9% 57.9% 0_1ins615;970_972delGACinsATT;975_1062delinsA
16 human 2710 GK glycerol kinase XM_011545493.2 57.9% 57.9% 0_1ins615;970_972delGACinsATT;975_1062delinsA
17 human 2710 GK glycerol kinase XM_011545494.2 57.9% 57.9% 0_1ins615;970_972delGACinsATT;975_1062delinsA
18 human 2710 GK glycerol kinase XM_017029409.1 57.9% 57.9% 0_1ins615;970_972delGACinsATT;975_1062delinsA
19 human 2710 GK glycerol kinase XM_005274488.4 56.8% 56.8% (many diffs)
20 mouse 14933 Gk glycerol kinase NM_001331046.1 91.4% 96.7% (many diffs)
21 mouse 14933 Gk glycerol kinase NM_008194.3 90.3% 95.8% (many diffs)
22 mouse 14933 Gk glycerol kinase XM_006527833.3 86.8% 91.9% (many diffs)
23 mouse 14933 Gk glycerol kinase NM_001294140.1 86.5% 91.5% (many diffs)
24 mouse 14933 Gk glycerol kinase NM_001294138.1 85.8% 91% (many diffs)
25 mouse 14933 Gk glycerol kinase NM_212444.2 85.5% 90.6% (many diffs)
26 mouse 14933 Gk glycerol kinase XM_006527829.3 82.3% 87.2% (many diffs)
27 mouse 14933 Gk glycerol kinase XM_006527831.3 81.4% 86.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1656
ORF length:
1590
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agcctcaaag aaggcagttt tggggccatt ggtgggggcg gtggaccagg
121gcaccagttc gacgcgcttt ttggttttca attcaaaaac agctgaacta cttagtcatc
181atcaagtaga aataaaacaa gagttcccaa gagaaggatg ggtggaacag gaccctaagg
241aaattctaca ttctgtctat gagtgtatag agaaaacatg tgagaaactt ggacagctca
301aaattgatat ttccaacata aaagctattg gtgtcagcaa ccagagggaa accactgtag
361tctgggacaa gataactgga gagcctctct acaatgctgt ggtgtggctt gatctaagaa
421cccagtctac cgttgagagt cttagtaaaa gaattacagg aaataataac tttgtcaagt
481ccaagacagg ccttccactt agcacttact tcagtgcagt gaaacttcgt tggctccttg
541acaatgtgag aaaagttcaa aaggcagttg aagaaaaacg agctcttttt gggactattg
601attcatggct tatttggagt ttgacaggag gagtcaatgg aggtgtccac tgtacagatg
661taacaaatgc aagtaggact atgcttttca acattcattc tttggaatgg gataaacaac
721tctgcgaatt ttttggaatt ccaatggaaa ttcttccaaa tgtccggagt tcttctgaga
781tctatggcct aatgaaaatc tctcatagcg tgaaagctgg ggccttggaa ggtgtgccaa
841tatctgggtg tttaggggac cagtctgctg cattggtggg acaaatgtgc ttccagattg
901gacaagccaa aaatacgtat ggaacaggat gtttcttact atgtaataca ggccataagt
961gtgtattttc tgatcatggc cttctcacca cagtggctta caaacttggc agagacaaac
1021cagtatatta tgctttggaa ggttctgtag ctatagctgg tgctgttatt cgctggctaa
1081gagacaatct tggaattata aagacctcag aagaaattga aaaacttgct aaagaagtag
1141gtacttctta tggctgctac ttcgtcccag cattttcggg gttatatgca ccttattggg
1201agcccagcgc aagagggata atctgtggac tcactcagtt caccaataaa tgccatattg
1261cttttgctgc attagaagct gtttgtttcc aaactcgaga gattttggat gccatgaatc
1321gagactgtgg aattccactc agtcatttgc aggtagatgg aggaatgacc agcaacaaaa
1381ttcttatgca gctacaagca gacattctgt atataccagt agtgaagccc tcaatgcccg
1441aaaccactgc actgggtgcg gctatggcgg caggggctgc agaaggagtc ggcgtatgga
1501gtctcgaacc cgaggatttg tctgccgtca cgatggagcg gtttgaacct cagattaatg
1561cggaggaaag tgaaattcgt tattctacat ggaagaaagc tgtgatgaag tcaatgggtt
1621gggttacaac tcaatctcca gaaagtggta ttccataccc aactttcttg tacaaagtgg
1681ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1741ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.