Construct: ORF ccsbBroad304_06992
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017976.1_s304c1, BRDN0000407574
- Derived from:
- ccsbBroadEn_06992
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SRC (6714)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06992
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6714 | SRC | SRC proto-oncogene, non-rec... | NM_005417.4 | 87.2% | 78.1% | (many diffs) |
| 2 | human | 6714 | SRC | SRC proto-oncogene, non-rec... | NM_198291.2 | 87.2% | 78.1% | (many diffs) |
| 3 | human | 6714 | SRC | SRC proto-oncogene, non-rec... | XM_011529013.2 | 87.2% | 78.1% | (many diffs) |
| 4 | human | 6714 | SRC | SRC proto-oncogene, non-rec... | XM_017028024.1 | 86.2% | 77.3% | (many diffs) |
| 5 | human | 6714 | SRC | SRC proto-oncogene, non-rec... | XM_017028025.1 | 86.2% | 77.3% | (many diffs) |
| 6 | human | 6714 | SRC | SRC proto-oncogene, non-rec... | XM_017028026.1 | 86.2% | 77.3% | (many diffs) |
| 7 | human | 6714 | SRC | SRC proto-oncogene, non-rec... | XM_017028027.2 | 86.2% | 77.3% | (many diffs) |
| 8 | mouse | 20779 | Src | Rous sarcoma oncogene | NM_001025395.2 | 77.5% | 77% | (many diffs) |
| 9 | mouse | 20779 | Src | Rous sarcoma oncogene | NM_009271.3 | 76.7% | 76.1% | (many diffs) |
| 10 | mouse | 20779 | Src | Rous sarcoma oncogene | XM_006499063.3 | 75.2% | 74.6% | (many diffs) |
| 11 | mouse | 20779 | Src | Rous sarcoma oncogene | XM_006499065.3 | 75.2% | 74.6% | (many diffs) |
| 12 | mouse | 20779 | Src | Rous sarcoma oncogene | XM_006499066.2 | 75.2% | 74.6% | (many diffs) |
| 13 | mouse | 20779 | Src | Rous sarcoma oncogene | XM_011239402.2 | 75.2% | 74.6% | (many diffs) |
| 14 | mouse | 20779 | Src | Rous sarcoma oncogene | XM_011239403.2 | 75.2% | 74.6% | (many diffs) |
| 15 | mouse | 20779 | Src | Rous sarcoma oncogene | XM_011239404.1 | 75.2% | 74.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1401
- ORF length:
- 1335
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg tagcaacaag agcaagccca aggatgccag ccagcggcgc cgcagcctgg 121 agcccgccga gaacgtgcac ggcgctggcg ggggcgcttt ccccgcctcg cagaccccca 181 gcaagccagc ctcggccgac ggccaccgcg gccccagcgc ggccttcgcc cccgcggccg 241 ccgagcccaa gctgttcgga ggcttcaact cctcggacac cgtcacctcc ccgcagaggg 301 cgggcccgct ggccggtgga gtgaccacct ttgtggccct ctatgactat gagtctagga 361 cggagacaga cctgtccttc aagaaaggcg agcggctcca gattgtcaac aacacagagg 421 gagactggtg gctggcccac tcgctcagca caggacagac aggctacatc cccagcaact 481 acgtggcgcc ctccgactcc atccaggctg aggagtggta ttttggcaag atcaccagac 541 gggagtcaga gcggttactg ctcaatgcag agaacccgag agggaccttc ctcgtgcgag 601 aaagtgagac cacgaaaggt gcctactgcc tctcagtgtc tgacttcgac aacgccaagg 661 gcctcaacgt gaagcactac aagatccgca agctggacag cggcggcttc tacatcacct 721 cccgcaccca gttcaacagc ctgcagcagc tggtggccta ctactccaaa cacgccgatg 781 gcctgtgcca ccgcctcacc accgtgtgcc ccacgtccaa gccgcagact cagggcctgg 841 ccaaggatgc ctgggagatc cctcgggagt cgctgcggct ggaggtcaag ctgggccagg 901 gctgctttgg cgaggtgtgg atggggacct ggaacggtac caccagggtg gccatcaaaa 961 ccctgaagcc tggcacgatg tctccagagg ccttcctgca ggaggcccag gtcatgaaga 1021 agctgaggca tgagaagctg gtgcagttgt atgctgtggt ttcagaggag cccatttaca 1081 tcgtcacgga gtacatgagc aaggggagtt tgctggactt tctcaagggg gagacaggca 1141 agtacctgcg gctgcctcag ctggtggaca tggctgctca gatcgcctca ggcatggcgt 1201 acgtggagcg gatgaactac gtccaccggg accttcgtgc agccaacatc ctggtgggag 1261 agaacctggt gtgcaaagtg gccgactttg ggctggctcg gctCTTAGAC GAACCACACA 1321 TGGAGTTAAG GACATGCAAG GTGTCGATTC CACAAACTAG TGCAAGTTCC AGAACCTGCC 1381 CTATATTTGA AATCAGCATC TAGTAGGAAC AGGGGTCTTT CTAGATCTTG TAAATGATTA 1441 AAGAAAAATA AAGGTTCCAG CGCATCAGAA AGGACGCAAA AGGTAGGAAC CCATAACAAG 1501 AAGAGCCAAC CTATATGGCC GAAAAAAATA CCGGAGTGCG CGCCCGCCCT CATGTGAGAT 1561 AAGTTGGAAG GAACCAGAGG AACGACCCAT TGGAAGATTA CCAGTCTTCG GTTAGTAGAA 1621 ATGCATTCAA AACCCCTCAT CCCAAGCGAT GGGGAACCTC TGGTAACCAA TTTTTTTGGA 1681 AGAGAGGGGA GGGCATCCCT ATCCCTATCT CTTTCTTCTT TTTTCTTTAT AGTTATAAGC 1741 AAGCGAGCGC CAACGGCGGG AGAAGTAGTC AATCAACCAC CCTATTACTT ACAAAGTTCT 1801 TTAAAACAAT ATTCATAAAA TGTATTCCTC CACATACATG TATATAGACA CCTTTAAACT 1861 TGTTATTAGT TATTTCTTTC CT