Transcript: Human XM_017028026.1

PREDICTED: Homo sapiens SRC proto-oncogene, non-receptor tyrosine kinase (SRC), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SRC (6714)
Length:
4117
CDS:
445..2073

Additional Resources:

NCBI RefSeq record:
XM_017028026.1
NBCI Gene record:
SRC (6714)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001149457 GACCTGGAACGGTACCACCA pXPR_003 GGG 895 55% 11 1.1207 SRC SRC 75652
2 BRDN0001146185 TCAATGCAGAGAACCCGAGA pXPR_003 GGG 531 33% 8 0.83 SRC SRC 75651
3 BRDN0001145402 TGTCCTTCAAGAAAGGCGAG pXPR_003 CGG 324 20% 5 0.3248 SRC SRC 75650
Download CSV

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017028026.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196395 GCGTCCATATTTAACATGTAA pLKO.1 3610 3UTR 100% 5.625 4.500 N SRC n/a
2 TRCN0000199186 CTGACTGAGCTCACCACAAAG pLKO.1 1825 CDS 100% 10.800 7.560 N SRC n/a
3 TRCN0000038150 GACAGACCTGTCCTTCAAGAA pLKO.1 744 CDS 100% 4.950 3.465 N SRC n/a
4 TRCN0000333003 GACAGACCTGTCCTTCAAGAA pLKO_005 744 CDS 100% 4.950 3.465 N SRC n/a
5 TRCN0000038153 GCGGCTCCAGATTGTCAACAA pLKO.1 771 CDS 100% 4.950 3.465 N SRC n/a
6 TRCN0000363725 GCGGCTCCAGATTGTCAACAA pLKO_005 771 CDS 100% 4.950 3.465 N SRC n/a
7 TRCN0000038149 GCTCGGCTCATTGAAGACAAT pLKO.1 1693 CDS 100% 4.950 3.465 N SRC n/a
8 TRCN0000333004 GCTCGGCTCATTGAAGACAAT pLKO_005 1693 CDS 100% 4.950 3.465 N SRC n/a
9 TRCN0000199313 GCTGACAGTTTGTGGCATCTT pLKO.1 3536 3UTR 100% 4.950 3.465 N SRC n/a
10 TRCN0000038152 TCAGAGCGGTTACTGCTCAAT pLKO.1 943 CDS 100% 4.950 3.465 N SRC n/a
11 TRCN0000038151 GTCATGAAGAAGCTGAGGCAT pLKO.1 1408 CDS 100% 2.640 1.848 N SRC n/a
12 TRCN0000195339 CATCCTCAGGAACCAACAATT pLKO.1 2832 3UTR 100% 13.200 7.920 N SRC n/a
13 TRCN0000344609 CATCCTCAGGAACCAACAATT pLKO_005 2832 3UTR 100% 13.200 7.920 N SRC n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017028026.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06992 pDONR223 99.9% 98.8% 98.8% None 351_368del;1236G>C n/a
2 TRCN0000472109 TAACCCCTCCATGAGAGGGTGTGC pLX_317 30.5% 98.8% 98.8% V5 351_368del;1236G>C n/a
3 ccsbBroad304_06992 pLX_304 22.9% 86.2% 77.3% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_14845 pDONR223 0% 98.8% 98.8% None 351_368del;1236G>C n/a
5 ccsbBroad304_14845 pLX_304 30.4% 98.8% 98.8% V5 351_368del;1236G>C n/a
6 TRCN0000470836 TCAGTGGTTTCCGGGGAGTTCTCG pLX_317 23.1% 98.8% 98.8% V5 351_368del;1236G>C n/a
7 TRCN0000488838 TAATATCATACATGAAACAATGTT pLX_317 16.6% 98.7% 98.7% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV