Construct: ORF ccsbBroad304_07509

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016457.1_s304c1, BRDN0000402013
Derived from:
ccsbBroadEn_07509
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMCC2 (9911)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07509
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9911 TMCC2 transmembrane and coiled-co... NM_014858.4 99.9% 100% 945A>G;1200C>T
2 human 9911 TMCC2 transmembrane and coiled-co... NM_001242925.1 88.9% 88.9% 0_1ins234;711A>G;966C>T
3 human 9911 TMCC2 transmembrane and coiled-co... XM_005245684.2 88.9% 88.9% 0_1ins234;711A>G;966C>T
4 human 9911 TMCC2 transmembrane and coiled-co... XM_005245685.4 88.9% 88.9% 0_1ins234;711A>G;966C>T
5 human 9911 TMCC2 transmembrane and coiled-co... XM_005245686.3 85.5% 84.4% (many diffs)
6 human 9911 TMCC2 transmembrane and coiled-co... NM_001331034.1 69.2% 66.9% (many diffs)
7 human 9911 TMCC2 transmembrane and coiled-co... NM_001297611.1 66.7% 65.6% (many diffs)
8 human 9911 TMCC2 transmembrane and coiled-co... NM_001297613.1 65.2% 65.1% (many diffs)
9 mouse 68875 Tmcc2 transmembrane and coiled-co... NM_178874.3 88.2% 94.2% (many diffs)
10 mouse 68875 Tmcc2 transmembrane and coiled-co... NM_001311107.1 78.2% 84.5% (many diffs)
11 mouse 68875 Tmcc2 transmembrane and coiled-co... XM_006529852.3 77.7% 80% (many diffs)
12 mouse 68875 Tmcc2 transmembrane and coiled-co... NM_001311108.1 58.1% 63.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2196
ORF length:
2127
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaagaggtgc agatcggacg agctgcagca acaacagggc gaggaggatg
121gagctgggct ggaagatgcc gcttcccacc tgccgggcgc ggacctccgg cctggggaga
181ccacgggtgc taactctgct ggcgggccaa cttcagacgc cggcgctgcc gcggcgccca
241acccaggtcc ccgaagcaag cctcctgatt taaagaaaat ccagcagctg tcagagggct
301ccatgtttgg ccacggtctg aagcacctgt tccacagccg ccgtcggtct cgggaaaggg
361agcaccagac gtctcaggat tcccagcagc atcagcagca gcagggtatg tccgaccatg
421actccccaga tgagaaggag cgctctccgg agatgcatcg cgtctcctac gccatgtccc
481tgcacgacct gcccgcccgg cccaccgcct tcaaccgcgt gctgcagcag atccgctccc
541ggccctccat caagcggggc gccagcctgc acagcagcag tgggggcggc agcagcggga
601gcagcagccg gcgcaccaag agtagctccc tggagcccca gcgtggcagc cctcacctgc
661tgcgcaaggc cccccaggac agcagcctgg ccgccatcct gcaccagcac cagtgccgtc
721cccgctcttc ctccaccacc gacactgctc tgctgctggc cgacggcagc aacgtgtacc
781tcctggctga ggaggccgaa ggcatcgggg acaaggtcga taagggagac ctggtggccc
841tgagcctccc cgccggccat ggtgacaccg acggccccat cagcctggac gtgcccgatg
901gggcaccgga cccccagcgg accaaggccg ccattgacca cctgcaccag aagatcctga
961agatcaccga gcagatcaag attgagcagg aggctcgcga cgacaatgtg gcggagtatc
1021tgaaactggc caacaacgcg gacaagcagc aggtgtcacg catcaagcaa gtgttcgaga
1081agaagaacca gaagtcagcc cagaccatcg cccagctgca caagaagctg gagcactacc
1141gccggcgcct gaaggagatt gagcagaacg ggccctcgcg gcagcccaag gacgtgctgc
1201gggacatgca gcaggggctg aaggacgtgg gcgccaacgt gcgcgcaggc atcagcggct
1261ttgggggtgg cgtggtggag ggcgtcaagg gcagcctctc tggcctctca caggccaccc
1321acaccgccgt ggtgtccaag ccccgggagt ttgccagcct catccgcaac aagtttggca
1381gtgctgacaa catcgcccac ctgaaggacc ccctggaaga tgggccccct gaggaggcag
1441cccgggcact gagcggcagt gccacactcg tctccagccc caagtatggc agcgatgatg
1501agtgctccag cgccagcgcc agctcagccg gggcaggcag caactctggg gctgggcctg
1561gtggggcgct ggggagccct aagtccaatg cactgtatgg tgctcctgga aacctggatg
1621ctctgctgga agagctacgg gagatcaagg agggacagtc tcacctggag gactccatgg
1681aagacctgaa gactcagctg cagagggact acacctacat gacccagtgc ctgcaggagg
1741agcgctacag gtacgagcgg ctggaggagc agctcaacga cctgactgag cttcatcaga
1801acgagatgac gaacctgaag caggagctgg ccagcatgga ggagaaggtg gcctaccagt
1861cctatgagag ggcacgggac atccaggagg ccgtggagtc ctgcctgacc cgggtcacca
1921agctggagct gcagcagcaa cagcagcagg tggtacagct ggagggcgtg gagaatgcca
1981acgcgcgggc gctgctgggc aagttcatca acgtgatcct ggcgctcatg gccgtgctgc
2041tggtgttcgt gtccaccatc gccaacttca tcacgcccct catgaagaca cgcctgcgca
2101tcaccagcac caccctcctg gtcctcgtcc tgttcctcct ctggaagcac tgggactccc
2161tcacctacct cctggagcac gtgttgctgc ccagcttgcc aactttcttg tacaaagtgg
2221ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
2281ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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