Construct: ORF ccsbBroadEn_07509
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016457.1_s300c1, BRDN0000395248
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMCC2 (9911)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9911 | TMCC2 | transmembrane and coiled-co... | NM_014858.4 | 99.9% | 100% | 945A>G;1200C>T |
| 2 | human | 9911 | TMCC2 | transmembrane and coiled-co... | NM_001242925.1 | 88.9% | 88.9% | 0_1ins234;711A>G;966C>T |
| 3 | human | 9911 | TMCC2 | transmembrane and coiled-co... | XM_005245684.2 | 88.9% | 88.9% | 0_1ins234;711A>G;966C>T |
| 4 | human | 9911 | TMCC2 | transmembrane and coiled-co... | XM_005245685.4 | 88.9% | 88.9% | 0_1ins234;711A>G;966C>T |
| 5 | human | 9911 | TMCC2 | transmembrane and coiled-co... | XM_005245686.3 | 85.5% | 84.4% | (many diffs) |
| 6 | human | 9911 | TMCC2 | transmembrane and coiled-co... | NM_001331034.1 | 69.2% | 66.9% | (many diffs) |
| 7 | human | 9911 | TMCC2 | transmembrane and coiled-co... | NM_001297611.1 | 66.7% | 65.6% | (many diffs) |
| 8 | human | 9911 | TMCC2 | transmembrane and coiled-co... | NM_001297613.1 | 65.2% | 65.1% | (many diffs) |
| 9 | mouse | 68875 | Tmcc2 | transmembrane and coiled-co... | NM_178874.3 | 88.2% | 94.2% | (many diffs) |
| 10 | mouse | 68875 | Tmcc2 | transmembrane and coiled-co... | NM_001311107.1 | 78.2% | 84.5% | (many diffs) |
| 11 | mouse | 68875 | Tmcc2 | transmembrane and coiled-co... | XM_006529852.3 | 77.7% | 80% | (many diffs) |
| 12 | mouse | 68875 | Tmcc2 | transmembrane and coiled-co... | NM_001311108.1 | 58.1% | 63.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2196
- ORF length:
- 2127
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GAAGAGGTGC AGATCGGACG AGCTGCAGCA ACAACAGGGC GAGGAGGATG 121 GAGCTGGGCT GGAAGATGCC GCTTCCCACC TGCCGGGCGC GGACCTCCGG CCTGGGGAGA 181 CCACGGGTGC TAACTCTGCT GGCGGGCCAA CTTCAGACGC CGGCGCTGCC GCGGCGCCCA 241 ACCCAGGTCC CCGAAGCAAG CCTCCTGATT TAAAGAAAAT CCAGCAGCTG TCAGAGGGCT 301 CCATGTTTGG CCACGGTCTG AAGCACCTGT TCCACAGCCG CCGTCGGTCT CGGGAAAGGG 361 AGCACCAGAC GTCTCAGGAT TCCCAGCAGC ATCAGCAGCA GCAGGGTATG TCCGACCATG 421 ACTCCCCAGA TGAGAAGGAG CGCTCTCCGG AGATGCATCG CGTCTCCTAC GCCATGTCCC 481 TGCACGACCT GCCCGCCCGG CCCACCGCCT TCAACCGCGT GCTGCAGCAG ATCCGCTCCC 541 GGCCCTCCAT CAAGCGGGGC GCCAGCCTGC ACAGCAGCAG TGGGGGCGGC AGCAGCGGGA 601 GCAGCAGCCG GCGCACCAAG AGTAGCTCCC TGGAGCCCCA GCGTGGCAGC CCTCACCTGC 661 TGCGCAAGGC CCCCCAGGAC AGCAGCCTGG CCGCCATCCT GCACCAGCAC CAGTGCCGTC 721 CCCGCTCTTC CTCCACCACC GACACTGCTC TGCTGCTGGC CGACGGCAGC AACGTGTACC 781 TCCTGGCTGA GGAGGCCGAA GGCATCGGGG ACAAGGTCGA TAAGGGAGAC CTGGTGGCCC 841 TGAGCCTCCC CGCCGGCCAT GGTGACACCG ACGGCCCCAT CAGCCTGGAC GTGCCCGATG 901 GGGCACCGGA CCCCCAGCGG ACCAAGGCCG CCATTGACCA CCTGCACCAG AAGATCCTGA 961 AGATCACCGA GCAGATCAAG ATTGAGCAGG AGGCTCGCGA CGACAATGTG GCGGAGTATC 1021 TGAAACTGGC CAACAACGCG GACAAGCAGC AGGTGTCACG CATCAAGCAA GTGTTCGAGA 1081 AGAAGAACCA GAAGTCAGCC CAGACCATCG CCCAGCTGCA CAAGAAGCTG GAGCACTACC 1141 GCCGGCGCCT GAAGGAGATT GAGCAGAACG GGCCCTCGCG GCAGCCCAAG GACGTGCTGC 1201 GGGACATGCA GCAGGGGCTG AAGGACGTGG GCGCCAACGT GCGCGCAGGC ATCAGCGGCT 1261 TTGGGGGTGG CGTGGTGGAG GGCGTCAAGG GCAGCCTCTC TGGCCTCTCA CAGGCCACCC 1321 ACACCGCCGT GGTGTCCAAG CCCCGGGAGT TTGCCAGCCT CATCCGCAAC AAGTTTGGCA 1381 GTGCTGACAA CATCGCCCAC CTGAAGGACC CCCTGGAAGA TGGGCCCCCT GAGGAGGCAG 1441 CCCGGGCACT GAGCGGCAGT GCCACACTCG TCTCCAGCCC CAAGTATGGC AGCGATGATG 1501 AGTGCTCCAG CGCCAGCGCC AGCTCAGCCG GGGCAGGCAG CAACTCTGGG GCTGGGCCTG 1561 GTGGGGCGCT GGGGAGCCCT AAGTCCAATG CACTGTATGG TGCTCCTGGA AACCTGGATG 1621 CTCTGCTGGA AGAGCTACGG GAGATCAAGG AGGGACAGTC TCACCTGGAG GACTCCATGG 1681 AAGACCTGAA GACTCAGCTG CAGAGGGACT ACACCTACAT GACCCAGTGC CTGCAGGAGG 1741 AGCGCTACAG GTACGAGCGG CTGGAGGAGC AGCTCAACGA CCTGACTGAG CTTCATCAGA 1801 ACGAGATGAC GAACCTGAAG CAGGAGCTGG CCAGCATGGA GGAGAAGGTG GCCTACCAGT 1861 CCTATGAGAG GGCACGGGAC ATCCAGGAGG CCGTGGAGTC CTGCCTGACC CGGGTCACCA 1921 AGCTGGAGCT GCAGCAGCAA CAGCAGCAGG TGGTACAGCT GGAGGGCGTG GAGAATGCCA 1981 ACGCGCGGGC GCTGCTGGGC AAGTTCATCA ACGTGATCCT GGCGCTCATG GCCGTGCTGC 2041 TGGTGTTCGT GTCCACCATC GCCAACTTCA TCACGCCCCT CATGAAGACA CGCCTGCGCA 2101 TCACCAGCAC CACCCTCCTG GTCCTCGTCC TGTTCCTCCT CTGGAAGCAC TGGGACTCCC 2161 TCACCTACCT CCTGGAGCAC GTGTTGCTGC CCAGCTTGCC AACTTTCTTG TACAAAGTtg 2221 gcattataag aaagcattgc ttatcaattt gttgcaacga ac