Transcript: Human NM_001242925.1

Homo sapiens transmembrane and coiled-coil domain family 2 (TMCC2), transcript variant 2, mRNA.

Source:
NCBI, updated 2018-06-25
Taxon:
Homo sapiens (human)
Gene:
TMCC2 (9911)
Length:
3357
CDS:
226..2121

Additional Resources:

NCBI RefSeq record:
NM_001242925.1
NBCI Gene record:
TMCC2 (9911)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001242925.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000431214 ACCGAGCAGATCAAGATTGAG pLKO_005 889 CDS 100% 4.950 6.930 N TMCC2 n/a
2 TRCN0000127974 GAACGAGATGACGAACCTGAA pLKO.1 1722 CDS 100% 4.050 5.670 N TMCC2 n/a
3 TRCN0000128931 CCTAAGTCCAATGCACTGTAT pLKO.1 1501 CDS 100% 4.950 3.465 N TMCC2 n/a
4 TRCN0000130626 CCTGACTGAGCTTCATCAGAA pLKO.1 1704 CDS 100% 4.950 3.465 N TMCC2 n/a
5 TRCN0000127687 GACGACAATGTGGCAGAGTAT pLKO.1 922 CDS 100% 4.950 3.465 N TMCC2 n/a
6 TRCN0000130364 GCAAGTGTTCGAGAAGAAGAA pLKO.1 990 CDS 100% 4.950 3.465 N TMCC2 n/a
7 TRCN0000127778 GTTTGGCAGTGCTGACAACAT pLKO.1 1296 CDS 100% 4.950 3.465 N TMCC2 n/a
8 TRCN0000127999 GCACCAGAAGATCCTGAAGAT pLKO.1 867 CDS 100% 4.950 2.970 N TMCC2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001242925.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07509 pDONR223 100% 88.9% 88.9% None 0_1ins234;711A>G;966C>T n/a
2 ccsbBroad304_07509 pLX_304 0% 88.9% 88.9% V5 0_1ins234;711A>G;966C>T n/a
3 ccsbBroadEn_15683 pDONR223 0% 44.3% 32.7% None (many diffs) n/a
4 ccsbBroad304_15683 pLX_304 0% 44.3% 32.7% V5 (many diffs) n/a
5 TRCN0000465348 TAGGGTCCGCGCCGTGCGTGGTAC pLX_317 32.9% 44.3% 32.7% V5 (many diffs) n/a
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