Construct: ORF ccsbBroad304_07792
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006185.1_s304c1, BRDN0000402461
- Derived from:
- ccsbBroadEn_07792
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL2 (11275)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07792
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11275 | KLHL2 | kelch like family member 2 | NM_007246.4 | 99.9% | 99.8% | 1024A>G |
| 2 | human | 11275 | KLHL2 | kelch like family member 2 | NM_001161521.1 | 98.7% | 97.9% | (many diffs) |
| 3 | human | 11275 | KLHL2 | kelch like family member 2 | XM_011531572.2 | 93.6% | 93.5% | 151_270del;1144A>G |
| 4 | human | 11275 | KLHL2 | kelch like family member 2 | XM_017007674.1 | 93.5% | 93.4% | 0_1ins114;910A>G |
| 5 | human | 11275 | KLHL2 | kelch like family member 2 | XM_024453881.1 | 93.5% | 93.4% | 0_1ins114;910A>G |
| 6 | human | 11275 | KLHL2 | kelch like family member 2 | NM_001161522.1 | 85.1% | 84.9% | 0_1ins264;760A>G |
| 7 | human | 11275 | KLHL2 | kelch like family member 2 | XM_017007676.1 | 85.1% | 84.9% | 0_1ins264;760A>G |
| 8 | human | 11275 | KLHL2 | kelch like family member 2 | NM_001331024.1 | 83.5% | 79.5% | 0_1ins169;85_86ins122;733A>G |
| 9 | human | 11275 | KLHL2 | kelch like family member 2 | XM_011531576.3 | 83.5% | 79.5% | 0_1ins169;85_86ins122;733A>G |
| 10 | human | 11275 | KLHL2 | kelch like family member 2 | NM_001331023.1 | 71.9% | 71.8% | 0_1ins498;526A>G |
| 11 | human | 11275 | KLHL2 | kelch like family member 2 | XM_017007675.2 | 69.7% | 69.1% | (many diffs) |
| 12 | human | 11275 | KLHL2 | kelch like family member 2 | XR_001741102.1 | 66.9% | (many diffs) | |
| 13 | human | 11275 | KLHL2 | kelch like family member 2 | XR_002959703.1 | 56.9% | (many diffs) | |
| 14 | human | 11275 | KLHL2 | kelch like family member 2 | XR_001741101.2 | 55% | (many diffs) | |
| 15 | human | 11275 | KLHL2 | kelch like family member 2 | XR_002959704.1 | 54.3% | (many diffs) | |
| 16 | human | 11275 | KLHL2 | kelch like family member 2 | XR_002959705.1 | 53.1% | (many diffs) | |
| 17 | mouse | 77113 | Klhl2 | kelch-like 2, Mayven | NM_178633.3 | 88.7% | 98.6% | (many diffs) |
| 18 | mouse | 77113 | Klhl2 | kelch-like 2, Mayven | XM_017313012.1 | 86.8% | 95.8% | (many diffs) |
| 19 | mouse | 77113 | Klhl2 | kelch-like 2, Mayven | XM_017313011.1 | 84.1% | 93.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1845
- ORF length:
- 1779
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gacgccgccg ctgcctcccg catgcacaaa gcagggtcat cagaagcctc 121 tcgattcaaa agatgataat accgaaaaac actgcccagt gacagtgaat ccttggcata 181 tgaagaaagc tttcaaagtc atgaacgaat taagaagtca aaatttgctg tgcgatgtca 241 caattgtggc agaagacatg gaaatttctg ctcatagagt ggtgctggcc gcctgtagtc 301 cttattttca tgccatgttt acaggtgaga tgagtgagag ccgagcaaag agagttagaa 361 taaaagaggt agatggctgg accctgagga tgctaattga ttatgtttac actgcagaaa 421 ttcaggttac agaagaaaat gtacaggtac ttctcccagc agctggtctc ttacagttac 481 aggatgtgaa gaagacttgt tgtgaatttt tggaatccca gcttcaccct gtcaactgct 541 taggaatccg ggcttttgct gatatgcatg catgtaccga ccttctgaac aaggccaaca 601 cctatgcaga gcaacatttt gcagatgttg tacttagtga agaatttctc aatcttggca 661 tcgaacaagt gtgcagctta atctcaagtg acaaacttac catttcttca gaagagaagg 721 tatttgaagc agtaatagca tgggtgaacc atgacaagga tgtgaggcaa gagtttatgg 781 cccgactgat ggaacatgta cggttacctt tgcttcctcg ggaatattta gttcagaggg 841 ttgaagagga agcattggtc aagaatagca gtgcttgcaa agattacctc attgaagcaa 901 tgaagtacca tttgctgcca acagagcagc gtatattaat gaagagtgtc cggacccggc 961 tgaggacacc catgaacctt cccaaattga tggtggtggt tgggggccaa gcaccaaagg 1021 ctatccggag tgtggaatgc tatgacttta aagaagaaag gtggcaccaa gtagcagagt 1081 tgccttccgg gaggtgcagg gcaggcatgg tctacatggc tggacttgtt tttgctgttg 1141 gtggctttaa tggctcatta agagttcgca ctgtagattc ctacgaccct gtgaaggacc 1201 agtggaccag cgttgctaac atgagagacc ggagaagcac tttgggagct gctgtgttaa 1261 atggattatt atacgctgtg ggaggctttg atgggagtac aggtttgtca tctgtggaag 1321 catacaacat aaagtctaat gagtggtttc atgtagctcc catgaataca aggaggagca 1381 gtgttggtgt gggtgttgtt ggaggtttgc tctatgctgt aggaggttat gatggagcat 1441 cacgtcagtg tcttagcaca gtagaatgct ataatgctac aacaaatgag tggacctata 1501 tagcagaaat gagcaccagg cggagtggag caggtgttgg tgtgttaaac aatttattgt 1561 atgctgtagg aggtcatgat ggccctttag tacgaaaaag tgttgaagta tatgatccca 1621 ccactaacgc atggagacag gttgcagata tgaacatgtg cagaagaaat gcaggagttt 1681 gtgcagttaa tggtctgtta tatgttgttg gaggggatga tggttcctgt aacttggcgt 1741 cagtagaata ttataaccca acaaccgata aatggacagt tgtgtcatcg tgtatgagca 1801 cagggagaag ttatgcaggg gtcacagtta ttgataaacc attatgccca actttcttgt 1861 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1921 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a