Construct: ORF ccsbBroad304_09038
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007262.1_s304c1, BRDN0000410174
- Derived from:
- ccsbBroadEn_09038
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC19A3 (80704)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09038
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80704 | SLC19A3 | solute carrier family 19 me... | NM_001371411.1 | 99.9% | 100% | 99A>G |
| 2 | human | 80704 | SLC19A3 | solute carrier family 19 me... | NM_001371412.1 | 99.9% | 100% | 99A>G |
| 3 | human | 80704 | SLC19A3 | solute carrier family 19 me... | NM_025243.4 | 99.9% | 100% | 99A>G |
| 4 | human | 80704 | SLC19A3 | solute carrier family 19 me... | XM_011511933.1 | 99.9% | 100% | 99A>G |
| 5 | human | 80704 | SLC19A3 | solute carrier family 19 me... | NM_001371413.1 | 91.7% | 89.9% | (many diffs) |
| 6 | human | 80704 | SLC19A3 | solute carrier family 19 me... | NM_001371414.1 | 91.7% | 89.9% | (many diffs) |
| 7 | human | 80704 | SLC19A3 | solute carrier family 19 me... | XM_017005032.1 | 87.8% | 87.9% | 99A>G;149_352del |
| 8 | human | 80704 | SLC19A3 | solute carrier family 19 me... | XM_017005033.1 | 87.8% | 87.9% | 99A>G;149_352del |
| 9 | human | 80704 | SLC19A3 | solute carrier family 19 me... | XM_017005034.2 | 87.8% | 87.9% | 99A>G;149_352del |
| 10 | human | 80704 | SLC19A3 | solute carrier family 19 me... | XM_017005031.1 | 87.1% | 87.1% | 1_15del;114A>G;164_367del |
| 11 | human | 80704 | SLC19A3 | solute carrier family 19 me... | XM_017005030.1 | 86% | 86.1% | 1_36del;135A>G;185_388del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1554
- ORF length:
- 1488
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ttgttacaga acttcactaa gcagttcctg gatttacccc actgtgatcc 121 tctgcttatt tggttttttc tccatgatga gaccctcaga accgttcctt atcccatatt 181 tatctggacc agataaaaac ctgaccagtg cagagataac aaatgagatc ttccccgttt 241 ggacatactc ctacctggtg ctgctgctgc ctgtgtttgt cctcaccgat tatgtccgct 301 acaagccagt catcatcttg caaggtatca gtttcatcat tacctggctg ctgctgttgt 361 ttggccaagg agtgaagacc atgcaggttg tagagttctt ctatgggatg gtcaccgccg 421 ccgaggtggc ctactacgcc tacatataca gcgtggtcag ccccgagcac taccagagag 481 tgagcggcta ctgcaggagc gtcacgctgg ccgcctacac agcagggtcg gtgctggctc 541 aactcttggt atccctggcg aacatgtcgt acttttacct caacgtcata tccttggcct 601 ctgtctccgt ggctttcctt ttctcacttt tcctaccaat gcccaagaaa agcatgtttt 661 ttcatgcaaa acccagcaga gaaataaaga agtcatcaag cgtgaatcca gtattagagg 721 aaactcacga aggtgaagca ccaggctgtg aagagcagaa acccacatca gaaatactca 781 gcacttcagg gaagctgaat aagggccagc tgaacagcct gaaaccaagc aatgtgactg 841 tggacgtttt tgtgcagtgg ttccaagatt tgaaggagtg ctactcctca aaacgtcttt 901 tctactggtc tctatggtgg gctttcgcca cagcaggttt taaccaggtt ttgaactatg 961 ttcaaatcct gtgggattac aaggcgccat cccaagattc ttccatctat aatggggccg 1021 tagaagctat tgcaaccttt ggaggggctg tggctgcctt tgcagtgggt tatgtgaaag 1081 tcaactggga ccttctggga gagctggctc tggtggtctt ctcagttgtc aatgccggtt 1141 ctttatttct catgcattac acagccaata tctgggcgtg ctatgctggc tatttgatat 1201 tcaagtccag ctatatgctt cttataacca tagcagtatt tcagattgca gttaatctga 1261 atgtggaacg ctatgccttg gtatttggaa tcaacacctt tattgccttg gtgattcaga 1321 ccatcatgac tgtgattgta gtagatcaga gagggctcaa cttgccagtc agcattcagt 1381 ttttagttta tgggagctat tttgcagtaa ttgctggaat tttcctaatg agaagcatgt 1441 atattaccta ctcaaccaaa tcccagaagg atgtacagag ccctgctcca agtgagaatc 1501 cagatgtgtc tcacccagag gaagagagta atatcatcat gtcaacaaaa ctctacccaa 1561 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1621 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta