Construct: ORF ccsbBroad304_10663

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013556.1_s304c1, BRDN0000460776
Derived from:
ccsbBroadEn_10663
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NME1-NME2 (654364)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10663
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 654364 NME1-NME2 NME1-NME2 readthrough NM_001018136.3 91.4% 91.4% 0_1ins75
2 human 654364 NME1-NME2 NME1-NME2 readthrough NR_037149.2 68.8% 1_253del;1130_1272del
3 human 4830 NME1 NME/NM23 nucleoside diphosp... NM_198175.1 57.7% 58.2% (many diffs)
4 human 4831 NME2 NME/NM23 nucleoside diphosp... NM_001018137.3 52% 52% 0_1ins420
5 human 4831 NME2 NME/NM23 nucleoside diphosp... NM_001018138.1 52% 52% 0_1ins420
6 human 4831 NME2 NME/NM23 nucleoside diphosp... NM_001018139.2 52% 52% 0_1ins420
7 human 4831 NME2 NME/NM23 nucleoside diphosp... NM_002512.4 52% 52% 0_1ins420
8 human 4830 NME1 NME/NM23 nucleoside diphosp... NM_000269.2 49.2% 45.8% (many diffs)
9 human 4831 NME2 NME/NM23 nucleoside diphosp... NM_001198682.2 28% 26.1% 0_1ins420;227_228ins113;246_247ins97
10 mouse 18103 Nme2 NME/NM23 nucleoside diphosp... NM_001077529.2 47% 51% (many diffs)
11 mouse 18103 Nme2 NME/NM23 nucleoside diphosp... NM_008705.5 47% 51% (many diffs)
12 mouse 18102 Nme1 NME/NM23 nucleoside diphosp... NM_008704.2 43.1% 46.9% (many diffs)
13 mouse 433968 Gm5566 predicted pseudogene 5566 XR_868740.1 42.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
945
ORF length:
876
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggtgctactg tctactttag ggatcgtctt tcaaggcgag gggcctccta
121tctcaagctg tgatacagga accatggcca actgtgagcg taccttcatt gcgatcaaac
181cagatggggt ccagcggggt cttgtgggag agattatcaa gcgttttgag cagaaaggat
241tccgccttgt tggtctgaaa ttcatgcaag cttccgaaga tcttctcaag gaacactacg
301ttgacctgaa ggaccgtcca ttctttgccg gcctggtgaa atacatgcac tcagggccgg
361tagttgccat ggtctgggag gggctgaatg tggtgaagac gggccgagtc atgctcgggg
421agaccaaccc tgcagactcc aagcctggga ccatccgtgg agacttctgc atacaagttg
481gcaggaccat ggccaacctg gagcgcacct tcatcgccat caagccggac ggcgtgcagc
541gcggcctggt gggcgagatc atcaagcgct tcgagcagaa gggattccgc ctcgtggcca
601tgaagttcct ccgggcctct gaagaacacc tgaagcagca ctacattgac ctgaaagacc
661gaccattctt ccctgggctg gtgaagtaca tgaactcagg gccggttgtg gccatggtct
721gggaggggct gaacgtggtg aagacaggcc gagtgatgct tggggagacc aatccagcag
781attcaaagcc aggcaccatt cgtggggact tctgcattca ggttggcagg aacatcattc
841atggcagtga ttcagtaaaa agtgctgaaa aagaaatcag cctatggttt aagcctgaag
901aactggttga ctacaagtct tgtgctcatg actgggtcta tgaattgcca actttcttgt
961acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1021gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)