Construct: ORF ccsbBroad304_12925
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000986.1_s304c1, BRDN0000408546
- Derived from:
- ccsbBroadEn_12925
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLCZ1 (89869)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12925
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | NM_001330769.1 | 100% | 100% | |
| 2 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_024449255.1 | 100% | 100% | |
| 3 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_017020183.1 | 88.7% | 88.1% | (many diffs) |
| 4 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_024449254.1 | 85.1% | 81.4% | (many diffs) |
| 5 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_017020182.1 | 83% | 78.9% | (many diffs) |
| 6 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_017020186.1 | 81.7% | 81.1% | (many diffs) |
| 7 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_017020184.1 | 79.1% | 78.5% | (many diffs) |
| 8 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | NM_001330774.2 | 78% | 74.6% | (many diffs) |
| 9 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_017020185.2 | 74.5% | 74% | (many diffs) |
| 10 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_024449256.1 | 74.5% | 74% | (many diffs) |
| 11 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_017020179.1 | 70.7% | 67.1% | (many diffs) |
| 12 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_017020180.1 | 70.7% | 67.1% | (many diffs) |
| 13 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_017020181.1 | 70.7% | 67.1% | (many diffs) |
| 14 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_024449253.1 | 70.7% | 67.1% | (many diffs) |
| 15 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | NM_033123.4 | 68.2% | 68.2% | 135_713del |
| 16 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_017020177.1 | 66.9% | 66.5% | (many diffs) |
| 17 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_017020178.1 | 65.8% | 62.2% | (many diffs) |
| 18 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_005253519.2 | 63.9% | 63.9% | 135_713del;949_1071del |
| 19 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XM_017020176.1 | 62.7% | 62.3% | (many diffs) |
| 20 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XR_001748912.1 | 49.7% | (many diffs) | |
| 21 | human | 89869 | PLCZ1 | phospholipase C zeta 1 | XR_001748913.1 | 49.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1314
- ORF length:
- 1245
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaatgaga tggtttttgt caaagattca ggatgacttc agaggtggaa 121 aaattaacct agaaaaaact cagaggttac ttgaaaaatt agatattcgg tgcagttata 181 ttcatgtgaa acagattttt aagacatctg actacccagt ggtgctctct ttagaaaatc 241 actgctccac tgcccaacaa gaagtaatgg cagacaattt gcaggctact tttggagagt 301 ccttgctttc tgatatgctt gatgattttc ctgatactct accatcacca gaggcactaa 361 aattcaaaat attagttaaa aataagaaaa taggaacctt aaaggaaacc catgaaagaa 421 aaggttctga taagcgtgga gacaatcaag acaaggaaac aggggtaaaa aagttacctg 481 gagtaatgct tttcaagaaa aagaagacca ggaagctaaa aattgctctg gccttatctg 541 atcttgtcat ttatacgaaa gctgagaaat tcaaaagctt tcaacattca agattatatc 601 agcaatttaa tgaaaataat tctattgggg agacacaagc ccgaaaactt tcaaaattgc 661 gagtccatga gtttattttt cacaccagga agttcattac cagaatatat cccaaagcaa 721 caagagcaga ctcttctaat tttaatcccc aagaattttg gaatataggt tgtcaaatgg 781 tggctttaaa tttccagacc cctggtctgc ccatggatct gcaaaatggg aaatttttgg 841 ataatggtgg ttctggatat attttgaaac cacatttctt aagagagagt aaatcatact 901 ttaacccaag taacataaaa gagggtatgc caattacact tacaataagg ctcatcagtg 961 gtatccagtt gcctcttact cattcatcat ctaacaaagg tgattcatta gtaattatag 1021 aagtttttgg tgttccaaat gatcaaatga agcagcagac tcgtgtaatt aaaaaaaatg 1081 cttttagtcc aagatggaat gaaacattca catttattat tcatgtccca gaattggcat 1141 tgatacgttt tgttgttgaa ggtcaaggtt taatagcagg aaatgaattt cttgggcaat 1201 atactttgcc acttctatgc atgaacaaag gttatcgtcg tattcctctg ttttccagaa 1261 tgggtgagag ccttgagcct gcttcactgt ttgtttatgt ttggtacgtc agattgccaa 1321 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1381 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta