Construct: ORF ccsbBroad304_14010

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004244.1_s304c1, BRDN0000407460
Derived from:
ccsbBroadEn_14010
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSTPIP2 (9050)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14010
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9050 PSTPIP2 proline-serine-threonine ph... XM_011526254.2 88.2% 69% (many diffs)
2 human 9050 PSTPIP2 proline-serine-threonine ph... NM_024430.4 87.5% 67.8% 4A>C;742_838del;1002_1003ins31
3 human 9050 PSTPIP2 proline-serine-threonine ph... XM_011526253.2 79.9% 60.6% (many diffs)
4 human 9050 PSTPIP2 proline-serine-threonine ph... XM_011526256.1 69.2% 49.6% (many diffs)
5 human 9050 PSTPIP2 proline-serine-threonine ph... XR_935264.2 30.7% (many diffs)
6 mouse 19201 Pstpip2 proline-serine-threonine ph... NM_013831.4 75.1% 60.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1002
ORF length:
936
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc gcgctcactg ttcaagggaa acttttggag tgcagacatc ctcagcacca
121tcggctatga caacattatc caacatctga acaatggccg caagaactgc aaagagtttg
181aagactttct aaaagaaagg gcagcaattg aagagaggta tggcaaagat ctgctcaacc
241tctctaggaa gaagccgtgt ggacagtctg aaatcaacac cctgaagcgg gcccttgaag
301tcttcaagca gcaagtagac aatgtggcac aatgtcacat tcagcttgca cagagtttaa
361gagaagaggc caggaagatg gaagaattca gggaaaagca aaaactacaa cgaaaaaaga
421cagagctcat aatggatgct atccataaac aaaagagctt acaattcaag aaaaccatgg
481atgcaaagaa gaactatgag cagaaatgcc gggacaaaga tgaggcagaa caggccgtca
541gccggagtgc caacctggtg aacccgaagc aacaagaaaa gctttttgtg aaactggcaa
601cttcaaagac cgcagtagag gactcagaca aagcatacat gctgcacatc ggcaccctgg
661ataaggtccg agaagagtgg cagagtgagc acatcaaggc ctgcgaggca tttgaggctc
721aagaatgtga acgaataaac ttcttccgga atgcattgtg gttacatgtg aatcagctgt
781cacaacaatg tgtcaccagt gatgaacacc catcatgtat gagaatttct actcctccca
841gaagaatgca gtcccagcag gaaaggctac agggcctaac ttggcaagga gaggacccct
901cccaattcct aaaagctcac cagatgatcc caattactct ttggttgatg actacagttt
961gctctatcag taaaatcaat gaaaccagag ctttttccgg ctacccaact ttcttgtaca
1021aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1081ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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