Construct: ORF ccsbBroad304_14033

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018246.1_s304c1, BRDN0000410445
Derived from:
ccsbBroadEn_14033
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
IQSEC1 (9922)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14033
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... NM_001330619.2 99.9% 99.8% 740A>N
2 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... XM_024453846.1 99.9% 99.8% 740A>N
3 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... NM_014869.7 84.4% 84.1% (many diffs)
4 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... XM_011534311.2 80.8% 80.5% 740A>N;2432_2481del;2493_3018del
5 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... XM_011534312.2 80.8% 80.5% 740A>N;2432_2481del;2493_3018del
6 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... XM_011534313.2 80.8% 80.5% 740A>N;2432_2481del;2493_3018del
7 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... XM_011534314.2 80.8% 80.5% 740A>N;2432_2481del;2493_3018del
8 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... XM_024453845.1 79.8% 79.5% (many diffs)
9 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... NM_001134382.3 73% 72.7% (many diffs)
10 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... XM_011534308.2 72.1% 71.8% (many diffs)
11 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... XM_011534306.3 72% 71.7% (many diffs)
12 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... XM_011534307.2 72% 71.7% (many diffs)
13 human 9922 IQSEC1 IQ motif and Sec7 domain Ar... XM_011534305.2 71.8% 71.5% (many diffs)
14 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_017321534.1 75.8% 81.9% (many diffs)
15 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 NM_001134383.1 74.7% 80.7% (many diffs)
16 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_006505992.2 72.4% 78.2% (many diffs)
17 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_017321532.1 72.4% 78.2% (many diffs)
18 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_017321533.1 72.4% 78.2% (many diffs)
19 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_006505991.3 70.1% 76% (many diffs)
20 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_006505990.3 68.3% 73.8% (many diffs)
21 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_011241302.2 67.3% 72.8% (many diffs)
22 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 NM_001134384.1 65.4% 70.6% (many diffs)
23 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_006505988.3 64.5% 69.7% (many diffs)
24 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_006505986.3 64.5% 69.6% (many diffs)
25 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_006505987.3 64.5% 69.6% (many diffs)
26 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_006505985.3 64.3% 69.4% (many diffs)
27 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_011241301.2 60.5% 65.3% (many diffs)
28 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_006505984.3 60.2% 65% (many diffs)
29 mouse 232227 Iqsec1 IQ motif and Sec7 domain 1 XM_006505983.3 59.7% 64.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2508
ORF length:
2442
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct agaacgaaag tatggggggc gcctggtaac ccgccatgcg gcccgcacca
121tccagacggc gtttcgccag taccagatga acaagaactt cgagcgcttg cgcagctcca
181tgtcagagaa ccgcatgtca cgccggattg tgctgtccaa catgaggatg cagttctcct
241ttgaggggcc tgagaaagtg cacagctcct acttcgaggg gaagcaggtc tcagtgacta
301acgacggctc ccagctggga gccctggtgt cccctgagtg tggtgacctc agcgagccca
361ccaccctcaa gtctccggcc ccctccagtg actttgcgga cgccatcacc gagctggagg
421acgccttctc taggcaagtg aaatcactgg ccgagtccat cgacgatgcc ctcaactgcc
481gcagcctgca cactgaggag gcaccggccc tggatgcggc gcgggcccgg gacaccgaac
541cccagacagc cctgcacggc atggaccacc gcaaactgga cgagatgacg gcctcgtaca
601gtgatgtcac cctgtacatc gatgaggagg agctgtcgcc ccctctgccc ctctcgcagg
661caggggaccg gccgtccagc accgagtcgg acctgcggct acgggctggg ggcgcagccc
721cagactactg ggccctggcc cacaaagagg acaaggctga cacggacacg agctgccgga
781gcacgccgtc gctggagcgg caggngcagc ggctgcgggt ggagcatctg ccgctgctca
841ccatcgagcc acccagcgac agctctgtgg accttagtga ccgctcggag cgggggtcac
901tcaagaggca gagtgcttac gagcgcagcc ttggcgggca gcagggcagt cccaagcatg
961gtccccacag cggcgccccc aagagcctcc cccgggagga gcctgagttg cggccccggc
1021cccccaggcc cctggacagc cacttggcca tcaatggctc agccaaccgg cagagcaagt
1081ctgagtcgga ctactcagac ggtgacaatg acagcatcaa cagcacgtcc aactccaacg
1141ataccatcaa ctgcagctcc gagtcatcgt cccgtgacag cctgcgggag cagacgctca
1201gcaagcagac ctaccacaag gaggcccgca acagctggga ctcgcctgcc tttagcaacg
1261atgtcatccg caagaggcac taccgcatcg gcctgaacct cttcaacaag aagcctgaga
1321agggagtcca gtacctcatc gagcgtggct ttgtgcccga cacgcccgtc ggggtggccc
1381acttcctgct gcagcgcaag ggcctcagcc ggcagatgat cggcgagttc ctgggcaacc
1441ggcagaagca gttcaaccgt gacgtgctcg actgcgtcgt ggacgagatg gacttctcta
1501ccatggagct ggatgaggcc ctcaggaaat tccaggcgca catccgtgtc caaggggagg
1561ctcagaaagt ggagcggctc atagaggcgt tcagccagcg ctactgcatc tgcaaccctg
1621gggtggtgcg gcaattccgg aacccagaca ccattttcat cctggccttc gccatcatcc
1681tgctgaacac cgacatgtac agccccaatg tcaagcccga gcggaaaatg aagctagagg
1741acttcatcaa gaacctccga ggtgtggacg atggtgagga cattccccgt gagatgctga
1801tggggatcta tgaacggatc cgtaagcgag agctaaagac caatgaggac catgtgtccc
1861aggtgcagaa ggtggagaag ctcattgtgg ggaaaaagcc gatcggatcc ctgcatcccg
1921ggctcggctg tgtgctctct ctgccccacc gtcggttggt ctgctactgc cggctctttg
1981aggttccaga cccaaacaag ccccagaaac tcggactaca ccagcgagaa atcttcctgt
2041tcaacgacct cctggtggtc accaagatct tccagaagaa gaagaactcg gtgacgtaca
2101gcttccgaca gtccttctcc ttgtacggca tgcaggtcct gctcttcgag aaccagtact
2161accccaatgg catccggctc acctcgtctg tccccggagc agatatcaaa gtgttaataa
2221acttcaacgc ccccaaccct caagaccgga agaaattcac cgatgacctg cgggagtcca
2281ttgcggaagt ccaagagatg gagaagcaca ggatagagtc ggagctcgag aagcagaaag
2341gcgtcgtgcg gcccagcatg tcccagtgct ctagcctcaa aaaggagtcg ggcaacggaa
2401cactgagccg ggcctgcctg gacgacagct atgccagcgg tgagggcctc aagcgcagcg
2461ccctcagcag ctccctgcgg gacctctcgg aagccggggt ccatcattac ccaactttct
2521tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
2581aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)