Construct: ORF ccsbBroad304_14638
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019036.1_s304c1, BRDN0000459835
- Derived from:
- ccsbBroadEn_14638
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGFR1 (2260)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14638
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174065.2 | 100% | 100% | |
| 2 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_015850.4 | 100% | 100% | |
| 3 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_023110.3 | 99.7% | 99.7% | 442_447delCGTATG |
| 4 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013224.2 | 99.7% | 99.7% | 1272_1273insGTAACA |
| 5 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013225.2 | 99.7% | 99.7% | 1272_1273insGTAACA |
| 6 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174063.2 | 99.5% | 99.5% | 442_447delCGTATG;1278_1279insGTAACA |
| 7 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174064.2 | 97.7% | 95.6% | (many diffs) |
| 8 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174067.1 | 96.1% | 96.1% | 1_99del |
| 9 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544446.2 | 95.9% | 95.9% | 1_99del;541_546delCGTATG |
| 10 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013220.1 | 95.8% | 95.8% | 1_99del;1371_1372insGTAACA |
| 11 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544447.2 | 95.6% | 95.6% | 1_99del;541_546delCGTATG;1377_1378insGTAACA |
| 12 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001354367.1 | 94.6% | 91% | (many diffs) |
| 13 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716307.1 | 94.6% | 91% | (many diffs) |
| 14 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716303.3 | 94.4% | 90.8% | (many diffs) |
| 15 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716304.1 | 94.4% | 90.8% | (many diffs) |
| 16 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013221.1 | 94.4% | 90.8% | (many diffs) |
| 17 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001354369.1 | 94.4% | 90.8% | (many diffs) |
| 18 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013222.2 | 94.1% | 90.6% | (many diffs) |
| 19 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_024447097.1 | 92.6% | 87.2% | (many diffs) |
| 20 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544444.1 | 91% | 87.6% | (many diffs) |
| 21 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544443.2 | 90.8% | 87.4% | (many diffs) |
| 22 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013219.1 | 90.8% | 87.4% | (many diffs) |
| 23 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544445.2 | 90.6% | 87.2% | (many diffs) |
| 24 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_023106.3 | 89.1% | 89.1% | 89_90ins267 |
| 25 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001174066.2 | 88.9% | 88.9% | 89_90ins267;175_180delCGTATG |
| 26 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_023105.3 | 88.9% | 88.9% | 89_90ins267;175_180delCGTATG |
| 27 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001354368.2 | 88.9% | 88.9% | 89_90ins267;1005_1006insGTAACA |
| 28 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544450.2 | 85.6% | 85.6% | 1_99del;188_189ins267 |
| 29 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013227.1 | 85.4% | 85.4% | 1_99del;188_189ins267;1104_1105insGTAACA |
| 30 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013226.1 | 85.2% | 85.2% | (many diffs) |
| 31 | human | 2260 | FGFR1 | fibroblast growth factor re... | NM_001354370.1 | 84% | 80.6% | (many diffs) |
| 32 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716314.2 | 84% | 80.6% | (many diffs) |
| 33 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716310.3 | 83.8% | 80.4% | (many diffs) |
| 34 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716311.1 | 83.8% | 80.4% | (many diffs) |
| 35 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_006716312.2 | 83.8% | 80.4% | (many diffs) |
| 36 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544451.1 | 82.2% | 76.9% | (many diffs) |
| 37 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544449.1 | 80.8% | 77.6% | (many diffs) |
| 38 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544448.1 | 80.7% | 77.4% | (many diffs) |
| 39 | human | 2260 | FGFR1 | fibroblast growth factor re... | XR_001745495.1 | 67.5% | (many diffs) | |
| 40 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013230.1 | 60.7% | 60.7% | 0_1ins960;312_313insGTAACA |
| 41 | human | 2260 | FGFR1 | fibroblast growth factor re... | XR_001745496.1 | 58.9% | (many diffs) | |
| 42 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013229.2 | 56.3% | 53.2% | (many diffs) |
| 43 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_011544452.2 | 47.1% | 37.5% | (many diffs) |
| 44 | human | 2260 | FGFR1 | fibroblast growth factor re... | XM_017013231.1 | 47% | 37.5% | (many diffs) |
| 45 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | NM_001079908.2 | 89.8% | 98.5% | (many diffs) |
| 46 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_006509013.1 | 89.8% | 98.5% | (many diffs) |
| 47 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | NM_010206.3 | 89.6% | 98.2% | (many diffs) |
| 48 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_006509010.2 | 89.6% | 98.2% | (many diffs) |
| 49 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_006509012.1 | 89.6% | 98.2% | (many diffs) |
| 50 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_011242120.1 | 89.4% | 98% | (many diffs) |
| 51 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | XM_011242121.1 | 80% | 88.2% | (many diffs) |
| 52 | mouse | 14182 | Fgfr1 | fibroblast growth factor re... | NM_001079909.2 | 79.8% | 88% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2529
- ORF length:
- 2460
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggagctgg aagtgcctcc tcttctgggc tgtgctggtc acagccacac 121 tctgcaccgc taggccgtcc ccgaccttgc ctgaacaagc ccagccctgg ggagcccctg 181 tggaagtgga gtccttcctg gtccaccccg gtgacctgct gcagcttcgc tgtcggctgc 241 gggacgatgt gcagagcatc aactggctgc gggacggggt gcagctggcg gaaagcaacc 301 gcacccgcat cacaggggag gaggtggagg tgcaggactc cgtgcccgca gactccggcc 361 tctatgcttg cgtaaccagc agcccctcgg gcagtgacac cacctacttc tccgtcaatg 421 tttcagatgc tctcccctcc tcggaggatg atgatgatga tgatgactcc tcttcagagg 481 agaaagaaac agataacacc aaaccaaacc ccgtagctcc atattggaca tccccagaaa 541 agatggaaaa gaaattgcat gcagtgccgg ctgccaagac agtgaagttc aaatgccctt 601 ccagtgggac cccaaacccc acactgcgct ggttgaaaaa tggcaaagaa ttcaaacctg 661 accacagaat tggaggctac aaggtccgtt atgccacctg gagcatcata atggactctg 721 tggtgccctc tgacaagggc aactacacct gcattgtgga gaatgagtac ggcagcatca 781 accacacata ccagctggat gtcgtggagc ggtcccctca ccggcccatc ctgcaagcag 841 ggttgcccgc caacaaaaca gtggccctgg gtagcaacgt ggagttcatg tgtaaggtgt 901 acagtgaccc gcagccgcac atccagtggc taaagcacat cgaggtgaat gggagcaaga 961 ttggcccaga caacctgcct tatgtccaga tcttgaagac tgctggagtt aataccaccg 1021 acaaagagat ggaggtgctt cacttaagaa atgtctcctt tgaggacgca ggggagtata 1081 cgtgcttggc gggtaactct atcggactct cccatcactc tgcatggttg accgttctgg 1141 aagccctgga agagaggccg gcagtgatga cctcgcccct gtacctggag atcatcatct 1201 attgcacagg ggccttcctc atctcctgca tggtggggtc ggtcatcgtc tacaagatga 1261 agagtggtac caagaagagt gacttccaca gccagatggc tgtgcacaag ctggccaaga 1321 gcatccctct gcgcagacag gtaacagtgt ctgctgactc cagtgcatcc atgaactctg 1381 gggttcttct ggttcggcca tcacggctct cctccagtgg gactcccatg ctagcagggg 1441 tctctgagta tgagcttccc gaagaccctc gctgggagct gcctcgggac agactggtct 1501 taggcaaacc cctgggagag ggctgctttg ggcaggtggt gttggcagag gctatcgggc 1561 tggacaagga caaacccaac cgtgtgacca aagtggctgt gaagatgttg aagtcggacg 1621 caacagagaa agacttgtca gacctgatct cagaaatgga gatgatgaag atgatcggga 1681 agcataagaa tatcatcaac ctgctggggg cctgcacgca ggatggtccc ttgtatgtca 1741 tcgtggagta tgcctccaag ggcaacctgc gggagtacct gcaggcccgg aggcccccag 1801 ggctggaata ctgctacaac cccagccaca acccagagga gcagctctcc tccaaggacc 1861 tggtgtcctg cgcctaccag gtggcccgag gcatggagta tctggcctcc aagaagtgca 1921 tacaccgaga cctggcagcc aggaatgtcc tggtgacaga ggacaatgtg atgaagatag 1981 cagactttgg cctcgcacgg gacattcacc acatcgacta ctataaaaag acaaccaacg 2041 gccgactgcc tgtgaagtgg atggcacccg aggcattatt tgaccggatc tacacccacc 2101 agagtgatgt gtggtctttc ggggtgctcc tgtgggagat cttcactctg ggcggctccc 2161 cataccccgg tgtgcctgtg gaggaacttt tcaagctgct gaaggagggt caccgcatgg 2221 acaagcccag taactgcacc aacgagctgt acatgatgat gcgggactgc tggcatgcag 2281 tgccctcaca gagacccacc ttcaagcagc tggtggaaga cctggaccgc atcgtggcct 2341 tgacctccaa ccaggagtac ctggacctgt ccatgcccct ggaccagtac tcccccagct 2401 ttcccgacac ccggagctct acgtgctcct caggggagga ttccgtcttc tctcatgagc 2461 cgctgcccga ggagccctgc ctgccccgac acccagccca gcttgccaat ggcggactca 2521 aacgccgctt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 2581 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 2641 tcaacctctg gatta