Construct: ORF ccsbBroad304_14712
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003391.1_s304c1, BRDN0000407975
- Derived from:
- ccsbBroadEn_14712
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFIA (4774)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14712
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4774 | NFIA | nuclear factor I A | NM_005595.5 | 99.7% | 99.7% | 1011A>T;1476_1478delTGAinsCCT |
| 2 | human | 4774 | NFIA | nuclear factor I A | XM_017001362.2 | 97.9% | 97.9% | (many diffs) |
| 3 | human | 4774 | NFIA | nuclear factor I A | XM_011541515.3 | 96.2% | 96.3% | 1011A>T;1074_1127del;1530_1532delTGAinsCCT |
| 4 | human | 4774 | NFIA | nuclear factor I A | NM_001134673.4 | 94.3% | 93.9% | (many diffs) |
| 5 | human | 4774 | NFIA | nuclear factor I A | NM_001145511.2 | 92.7% | 92.1% | (many diffs) |
| 6 | human | 4774 | NFIA | nuclear factor I A | XM_011541512.3 | 91.1% | 90.7% | (many diffs) |
| 7 | human | 4774 | NFIA | nuclear factor I A | XM_011541514.3 | 89.5% | 88.9% | (many diffs) |
| 8 | human | 4774 | NFIA | nuclear factor I A | NM_001145512.2 | 86.8% | 86.2% | (many diffs) |
| 9 | human | 4774 | NFIA | nuclear factor I A | XM_017001363.1 | 43.2% | 42.1% | (many diffs) |
| 10 | mouse | 18027 | Nfia | nuclear factor I/A | XM_006502843.2 | 93.5% | 98.9% | (many diffs) |
| 11 | mouse | 18027 | Nfia | nuclear factor I/A | XM_006502841.3 | 88.8% | 93.2% | (many diffs) |
| 12 | mouse | 18027 | Nfia | nuclear factor I/A | NM_010905.3 | 88.5% | 93.1% | (many diffs) |
| 13 | mouse | 18027 | Nfia | nuclear factor I/A | XM_006502842.3 | 86.8% | 91.3% | (many diffs) |
| 14 | mouse | 18027 | Nfia | nuclear factor I/A | XM_006502845.2 | 85.4% | 90.7% | (many diffs) |
| 15 | mouse | 18027 | Nfia | nuclear factor I/A | NM_001122952.1 | 84.2% | 87.7% | (many diffs) |
| 16 | mouse | 18027 | Nfia | nuclear factor I/A | NM_001122953.1 | 80.6% | 85% | (many diffs) |
| 17 | mouse | 18027 | Nfia | nuclear factor I/A | XM_006502844.3 | 76.6% | 80% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1560
- ORF length:
- 1494
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgta ttctccgctc tgtctcaccc aggatgaatt tcatcctttc atcgaagcac 121 ttctgcccca cgtccgagcc tttgcctaca catggttcaa cctgcaggcc cgaaaacgaa 181 aatacttcaa aaaacatgaa aagcgtatgt caaaagaaga agagagagcc gtgaaggatg 241 aattgctaag tgaaaaacca gaggtcaagc agaagtgggc atctcgactt ctggcaaagt 301 tgcggaaaga tatccgaccc gaatatcgag aggattttgt tcttacagtt acagggaaaa 361 aacctccatg ttgtgttctt tccaacccag accagaaagg caagatgcga agaattgact 421 gcctccgcca ggcagataaa gtctggaggt tggaccttgt tatggtgatt ttgtttaaag 481 gtattccgct ggaaagtact gatggcgagc gccttgtaaa gtccccacaa tgctctaatc 541 cagggctctg tgtccaaccc catcacatag gggtttctgt taaggaactc gatttatatt 601 tggcatactt tgtgcatgca gcagattcaa gtcaatctga aagtcccagc cagccaagtg 661 acgctgacat taaggaccag ccagaaaatg gacatttggg cttccaggac agttttgtca 721 catcaggtgt ttttagtgtc actgagctag taagagtgtc acagacacca atagctgcag 781 gaactggccc aaatttttct ctctcagatt tggaaagttc ttcatactac agcatgagtc 841 caggagcaat gaggaggtct ttacccagca catcctctac gagctccaca aagcgcctca 901 agtctgtgga ggatgaaatg gacagtcctg gtgaggagcc attttataca ggccaagggc 961 gctccccagg aagtggcagt cagtcaagtg gatggcatga agtggagcca ggaatgccat 1021 ctccaaccac actgaagaag tcggagaagt ctggtttcag cagcccctcc ccttctcaga 1081 cctcctccct gggaacggcg ttcacacagc atcaccgacc tgtcattaca ggacccagag 1141 caagtccgca tgcaacacca tcgactcttc atttcccgac atcacccatt atccagcagc 1201 ctgggcctta cttctcacac ccagccatcc gctatcaccc tcaggagacg ctgaaagaat 1261 ttgtccaact tgtctgccct gatgctggtc agcaggctgg acaggtgggg ttcctcaatc 1321 ccaatgggag cagccaaggc aaggtgcaca acccattcct tcccacccca atgttgccac 1381 cgccaccgcc accaccgatg gccaggcctg tgcctctgcc ggtgccagac acaaagcctc 1441 caaccacgtc aacagaagga ggtgcagcct cccccacgtc accaatcctg gtacctggga 1501 taaaagttgc agcgtcccac catccaccag acagaccacc cctccccttc tcaactctgt 1561 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1621 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1681 ggatta