Construct: ORF ccsbBroad304_14790
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018987.1_s304c1, BRDN0000459470
- Derived from:
- ccsbBroadEn_14790
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKCE (5581)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14790
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5581 | PRKCE | protein kinase C epsilon | NM_005400.3 | 99.5% | 72.4% | (many diffs) |
| 2 | human | 5581 | PRKCE | protein kinase C epsilon | XM_005264428.1 | 99.5% | 72.4% | (many diffs) |
| 3 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532971.3 | 85.6% | 56.7% | (many diffs) |
| 4 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532975.3 | 85.6% | 56.7% | (many diffs) |
| 5 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004486.2 | 85.6% | 56.7% | (many diffs) |
| 6 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532978.2 | 83.8% | 56.7% | (many diffs) |
| 7 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004487.2 | 83.8% | 56.7% | (many diffs) |
| 8 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004488.2 | 83.8% | 56.7% | (many diffs) |
| 9 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532980.3 | 79.1% | 52.1% | (many diffs) |
| 10 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532981.3 | 79.1% | 52.1% | (many diffs) |
| 11 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004489.2 | 79.1% | 52.1% | (many diffs) |
| 12 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004490.2 | 79.1% | 52.1% | (many diffs) |
| 13 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004491.2 | 79.1% | 52.1% | (many diffs) |
| 14 | human | 5581 | PRKCE | protein kinase C epsilon | XM_024452991.1 | 79.1% | 52.1% | (many diffs) |
| 15 | human | 5581 | PRKCE | protein kinase C epsilon | XM_024452992.1 | 79.1% | 52.1% | (many diffs) |
| 16 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532982.2 | 67.2% | 40.5% | (many diffs) |
| 17 | human | 5581 | PRKCE | protein kinase C epsilon | XM_005264431.4 | 65.5% | 86.2% | (many diffs) |
| 18 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004492.2 | 64.7% | 85.9% | (many diffs) |
| 19 | human | 5581 | PRKCE | protein kinase C epsilon | XM_006712050.3 | 62.3% | 35.8% | 0_1ins831;699G>T;784_785insT |
| 20 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532983.2 | 58.1% | 30.6% | (many diffs) |
| 21 | human | 5581 | PRKCE | protein kinase C epsilon | XR_939695.2 | 54.5% | (many diffs) | |
| 22 | human | 5581 | PRKCE | protein kinase C epsilon | XR_001738846.2 | 17.8% | (many diffs) | |
| 23 | mouse | 18754 | Prkce | protein kinase C, epsilon | NM_011104.3 | 90.7% | 70.9% | (many diffs) |
| 24 | mouse | 18754 | Prkce | protein kinase C, epsilon | XM_017317328.1 | 85.3% | 75.4% | (many diffs) |
| 25 | mouse | 18754 | Prkce | protein kinase C, epsilon | XM_006523833.2 | 74% | 52.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1716
- ORF length:
- 1647
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtagtgttc aatggccttc ttaagatcaa aatctgcgag gccgtgagct 121 tgaagcccac agcctggtcg ctgcgccatg cggtgggacc ccggccgcag actttccttc 181 tcgaccccta cattgccctc aatgtggacg actcgcgcat cggccaaacg gccaccaagc 241 agaagaccaa cagcccggcc tggcacgacg agttcgtcac cgatgtgtgc aacggacgca 301 agatcgagct ggctgtcttt cacgatgccc ccataggcta cgacgacttc gtggccaact 361 gcaccatcca gtttgaggag ctgctgcaga acgggagccg ccacttcgag gactggattg 421 atctggagcc agaaggaaga gtgtatgtga tcatcgatct ctcagggtcg tcgggtgaag 481 cccctaaaga caatgaagag cgtgtgttca gggaacgcat gcggccgagg aagcggcagg 541 gggccgtcag gcgcagggtc catcaggtca acggccacaa gttcatggcc acctatcttc 601 ggcagcccac ctactgctcc cattgcagag acttcatctg gggtgtcata ggaaagcagg 661 gataccagtg tcaagtctgc acctgcgtgg tccacaagcg gtgccacgag ctcataatca 721 caaagtgtgc tggggttaag aagcaggaaa cccccgaaca ggtgggctcc cagcggttca 781 accgtcacat ggccccaaag ttcggtatcc acaactacaa ggtccctacc ttctgcgatc 841 actgtgggtc cctgctctgg ggactcttgc ggcagggttt gcagtgtaaa gtctgcaaaa 901 tgaatgttca ccgtcgatgt gagaccaacg tggctcccaa ctgtggagtg gatgccagag 961 gaatcgccaa agtactggcc gacctgggcg ttaccccaga caaaatcacc aacagcggcc 1021 agagaaggaa aaagctcatt gctggtgccg agtccccgca gcctgcttct ggaagctcac 1081 catctgagga agatcgatcc aagtcagcac ccacctcccc ttgtgaccag gaaataaaag 1141 aacttgagaa caacattcgg aaagccttgt catttgacaa ccgaggagag gagcaccggg 1201 cagcatcgtc tcctgatggc cagctgatga gccccggtga gaatggcgaa gtccggcaag 1261 gccaggccaa gcgcctgggc ctggatgagt tcaacttcat caaggtgttg ggcaaaggca 1321 gctttggcaa ggtcatgttg gcagaactca agggcaaaga tgaagtatat gctgtgaagg 1381 tcttaaagaa ggacgtcatc cttcaggatg atgacgtgga ctgcacaatg acagagaaga 1441 ggattttggc tctggcacgg aaacacccgt accttaccca actctactgc tgcttccaga 1501 ccaaggaccg cctctttttc gtcatggaat atgtaaatgg tggagacctc atgtttcaga 1561 ttcagcgctc ccgaaaattc gacgagcctc gttcacgttt ctatgcTGCA GAGGTCACAT 1621 CGGCCCTCAT GTTCCTCCAC CAGCATGGAG TCATCTACAG GGATTTGAAA CTGGACAACA 1681 TCCTTTCTGG ATGCAGAAGG TCACTGCAAG CTGGCTGACT TCGGGATGTG CAAGGAAGGG 1741 ATTCTGAATG GTGTGACGAC CACCACGTTC TGTGGGACTC CTGACTACAT AGCTCCTGAG 1801 ATCCTGCAGG AGTTGGAGTA TGGCCCCTCC GTGGACTGGT GGGCCCTGGG GGTGCTGATG 1861 TACGAGATGA TGGCTGGACA GCCTCCCTTT GAGGCCGACA ATGAGGACGA CCTATTTGAG 1921 TCCATCCTCC ATGACGACGT GCTGTACCCA GTCTGGCTCA GCAAGGAGGC TGTCAGCATC 1981 TTGAAAGCTT TCATGACGAA GAATCCCCAC AAGCGCCTGG GCTGTGTGGC ATCGCAGAAT 2041 GGCGAGGACG CCATCAAGCA GCACCCATTC TTCAAAGAGA TTGACTGGGT GCTCCTGGAG 2101 CAGAAGAAGA TCAAGCCACC CTTCAAACCA CGCATTAAAA CCAAAAGAGA CGTCAATAAT 2161 TTTGACCAAG ACTTTACCCG GGAAGAGCCG GTACTCACCC TTGTGGACGA AGCAATTGTA 2221 AAGCAGATCA ACCAGGAGGA ATTCAAAGGT TTCTCCTACT TTGGTGAAGA CCTGATGCCC 2281 TTGCCAACTT TCTTGTACaa agtggttggt aagcctatcc ctaaccctct cctcggtctc 2341 gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc 2401 tggatta