Construct: ORF ccsbBroadEn_14790
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018987.1_s300c1, BRDN0000458283
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKCE (5581)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5581 | PRKCE | protein kinase C epsilon | NM_005400.3 | 99.4% | 69.9% | (many diffs) |
| 2 | human | 5581 | PRKCE | protein kinase C epsilon | XM_005264428.1 | 99.4% | 69.9% | (many diffs) |
| 3 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532971.3 | 85.5% | 54.3% | (many diffs) |
| 4 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532975.3 | 85.5% | 54.3% | (many diffs) |
| 5 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004486.2 | 85.5% | 54.3% | (many diffs) |
| 6 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532978.2 | 83.7% | 54.2% | (many diffs) |
| 7 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004487.2 | 83.7% | 54.2% | (many diffs) |
| 8 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004488.2 | 83.7% | 54.2% | (many diffs) |
| 9 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532980.3 | 79% | 49.6% | (many diffs) |
| 10 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532981.3 | 79% | 49.6% | (many diffs) |
| 11 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004489.2 | 79% | 49.6% | (many diffs) |
| 12 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004490.2 | 79% | 49.6% | (many diffs) |
| 13 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004491.2 | 79% | 49.6% | (many diffs) |
| 14 | human | 5581 | PRKCE | protein kinase C epsilon | XM_024452991.1 | 79% | 49.6% | (many diffs) |
| 15 | human | 5581 | PRKCE | protein kinase C epsilon | XM_024452992.1 | 79% | 49.6% | (many diffs) |
| 16 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532982.2 | 67.1% | 38.1% | (many diffs) |
| 17 | human | 5581 | PRKCE | protein kinase C epsilon | XM_005264431.4 | 65.5% | 86.2% | (many diffs) |
| 18 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004492.2 | 64.7% | 85.9% | (many diffs) |
| 19 | human | 5581 | PRKCE | protein kinase C epsilon | XM_006712050.3 | 62.2% | 33.4% | (many diffs) |
| 20 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532983.2 | 58% | 28.3% | (many diffs) |
| 21 | human | 5581 | PRKCE | protein kinase C epsilon | XR_939695.2 | 54.3% | (many diffs) | |
| 22 | human | 5581 | PRKCE | protein kinase C epsilon | XR_001738846.2 | 17.8% | (many diffs) | |
| 23 | mouse | 18754 | Prkce | protein kinase C, epsilon | NM_011104.3 | 90.6% | 68.4% | (many diffs) |
| 24 | mouse | 18754 | Prkce | protein kinase C, epsilon | XM_017317328.1 | 85.3% | 72.7% | (many diffs) |
| 25 | mouse | 18754 | Prkce | protein kinase C, epsilon | XM_006523833.2 | 73.9% | 50.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 106
- ORF end:
- 1753
- ORF length:
- 1647
- Sequence:
-
1 CCCCAAATAA GTGATTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCACA AATTGATGAG 61 CAATGCTTTT TTATAATGCC AACTTTGTAC AAAAAAGTTG GCACCATGGT AGTGTTCAAT 121 GGCCTTCTTA AGATCAAAAT CTGCGAGGCC GTGAGCTTGA AGCCCACAGC CTGGTCGCTG 181 CGCCATGCGG TGGGACCCCG GCCGCAGACT TTCCTTCTCG ACCCCTACAT TGCCCTCAAT 241 GTGGACGACT CGCGCATCGG CCAAACGGCC ACCAAGCAGA AGACCAACAG CCCGGCCTGG 301 CACGACGAGT TCGTCACCGA TGTGTGCAAC GGACGCAAGA TCGAGCTGGC TGTCTTTCAC 361 GATGCCCCCA TAGGCTACGA CGACTTCGTG GCCAACTGCA CCATCCAGTT TGAGGAGCTG 421 CTGCAGAACG GGAGCCGCCA CTTCGAGGAC TGGATTGATC TGGAGCCAGA AGGAAGAGTG 481 TATGTGATCA TCGATCTCTC AGGGTCGTCG GGTGAAGCCC CTAAAGACAA TGAAGAGCGT 541 GTGTTCAGGG AACGCATGCG GCCGAGGAAG CGGCAGGGGG CCGTCAGGCG CAGGGTCCAT 601 CAGGTCAACG GCCACAAGTT CATGGCCACC TATCTTCGGC AGCCCACCTA CTGCTCCCAT 661 TGCAGAGACT TCATCTGGGG TGTCATAGGA AAGCAGGGAT ACCAGTGTCA AGTCTGCACC 721 TGCGTGGTCC ACAAGCGGTG CCACGAGCTC ATAATCACAA AGTGTGCTGG GGTTAAGAAG 781 CAGGAAACCC CCGAACAGGT GGGCTCCCAG CGGTTCAACC GTCACATGGC CCCAAAGttc 841 ggtatccaca actacaaggt ccctaccttc tgcgatcact gtgggtccct gctctgggga 901 ctcttgcggc agggtttgca gtgtaaagtc tgcaaaatga atgttcaccg tcgatgtgag 961 accaacgtgg ctcccaactg tggagtggat gccagaggaa tcgccaaagt actggccgac 1021 ctgggcgtta ccccagacaa aatcaccaac agcggccaga gaaggaaaaa gctcattgct 1081 ggtgccgagt ccccgcagcc tgcttctgga agctcaccat ctgaggaaga tcgatccaag 1141 tcagcaccca cctccccttg tgaccaggaa ataaaagaac ttgagaacaa cattcggaaa 1201 gccttgtcat ttgacaaccg aggagaggag caccgggcag catcgtctcc tgatggccag 1261 ctgatgagcc ccggtgagaa tggcgaagtc cggcaaggcc aggccaagcg cctgggcctg 1321 gatgagttca acttcatcaa ggtgttgggc aaaggcagct ttggcaaggt catgttggca 1381 gaactcaagg gcaaagatga agtatatgct gtgaaggtct taaagaagga cgtcatcctt 1441 caggatgatg acgtggactg cacaatgaca gagaagagga ttttggctct ggcacggaaa 1501 cacccgtacc ttacccaact ctactgctgc ttccagacca aggaccgcct ctttttcgtc 1561 atggaatatg taaatggtgg agacctcatg tttcagattc agcgctcccg aaaattcgac 1621 gagCCTCGTT CACGTTTCTA TGCTGCAGAG GTCACATCGG CCCCTCATGT TCTTCCACCA 1681 GCATGGAGTC ATCTACAGGG ATTTGAAACT GGACAACATC TTTCTGGATG CAGAAGGTCA 1741 CTGCAAGCTG GCTGACTTCG GGATGTGCAA GGAAGGGATT CTGAATGGTG TGACGACCAC 1801 CACGTTCTGT GGGACTCCTG ACTACATAGC TCCTGAGATC CTGCAGGAGT TGGAGTATGG 1861 CCCCTCCGTG GACTGGTGGG CCCTGGGGGT GCTGATGTAC GAGATGATGG CTGGACAGCC 1921 TCCCTTTGAG GCCGACAATG AGGACGACCT ATTTGAGTCC ATCCTCCATG ACGACGTGCT 1981 GTACCCAGTC TGGCTCAGCA AGGAGGCTGT CAGCATCTTG AAAGCTTTCA TGACGAAGAA 2041 TCCCCACAAG CGCCTGGGCT GTGTGGCATC GCAGAATGGC GAGGACGCCA TCAAGCAGCA 2101 CCCATTCTTC AAAGAGATTG ACTGGGTGCT CCTGGAGCAG AAGAAGATCA AGCCACCCTT 2161 CAAACCACGC ATTAAAACCA AAAGAGACGT CAATAATTTT GACCAAGACT TTACCCGGGA 2221 AGAGCCGGTA CTCACCCTTG TGGACGAAGC AATTGTAAAG CAGATCAACC AGGAGGAATT 2281 CAAAGGTTTC TCCTACTTTG GTGAAGACCT GATGCCCTTG CCAACTTTCT TGTACAAAGT 2341 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 2401 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 2461 CATAGCTGTT TC