Construct: ORF ccsbBroad304_14796

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000888.2_s304c1, BRDN0000459640
Derived from:
ccsbBroadEn_14796
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKCZ (5590)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14796
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5590 PRKCZ protein kinase C zeta NM_002744.6 100% 100%
2 human 5590 PRKCZ protein kinase C zeta XM_017001790.1 98.6% 98.6% 974_997del
3 human 5590 PRKCZ protein kinase C zeta XM_011541773.1 91.2% 86.1% (many diffs)
4 human 5590 PRKCZ protein kinase C zeta XM_017001789.1 90.1% 85% (many diffs)
5 human 5590 PRKCZ protein kinase C zeta NM_001242874.2 82.2% 81.4% (many diffs)
6 human 5590 PRKCZ protein kinase C zeta XM_011541774.1 74.1% 68.5% (many diffs)
7 human 5590 PRKCZ protein kinase C zeta XR_002957131.1 73.8% 1_157del;1732_1814del;2017_2405del
8 human 5590 PRKCZ protein kinase C zeta XR_002957130.1 73.1% (many diffs)
9 human 5590 PRKCZ protein kinase C zeta NM_001033581.2 69% 69% 0_1ins549
10 human 5590 PRKCZ protein kinase C zeta NM_001033582.2 69% 69% 0_1ins549
11 human 5590 PRKCZ protein kinase C zeta XM_017001801.1 68.4% 68.4% 0_1ins561
12 human 5590 PRKCZ protein kinase C zeta NM_001350803.1 68.1% 68.1% 0_1ins549;425_448del
13 human 5590 PRKCZ protein kinase C zeta NM_001350804.1 68.1% 68.1% 0_1ins549;425_448del
14 human 5590 PRKCZ protein kinase C zeta NR_146911.1 66.6% (many diffs)
15 human 5590 PRKCZ protein kinase C zeta XM_011541775.3 66.5% 56.3% (many diffs)
16 human 5590 PRKCZ protein kinase C zeta XM_011541776.1 61.5% 56.8% (many diffs)
17 human 5590 PRKCZ protein kinase C zeta XM_024448396.1 61.5% 56.8% (many diffs)
18 human 5590 PRKCZ protein kinase C zeta XM_017001800.1 60.9% 56.2% (many diffs)
19 human 5590 PRKCZ protein kinase C zeta XM_017001792.1 60.7% 56.1% (many diffs)
20 human 5590 PRKCZ protein kinase C zeta XM_017001793.1 60.7% 56.1% (many diffs)
21 human 5590 PRKCZ protein kinase C zeta XM_024448391.1 60.7% 56.1% (many diffs)
22 human 5590 PRKCZ protein kinase C zeta XM_017001797.1 60.1% 55.5% (many diffs)
23 human 5590 PRKCZ protein kinase C zeta NM_001350805.1 59.9% 59.9% 0_1ins711
24 human 5590 PRKCZ protein kinase C zeta NM_001350806.1 59.9% 59.9% 0_1ins711
25 human 5590 PRKCZ protein kinase C zeta XM_017001803.2 58.1% 56.2% (many diffs)
26 human 5590 PRKCZ protein kinase C zeta XR_001737315.2 57.6% (many diffs)
27 human 5590 PRKCZ protein kinase C zeta XM_017001802.1 52.8% 48.2% (many diffs)
28 human 5590 PRKCZ protein kinase C zeta XM_024448397.1 52.1% 47.6% (many diffs)
29 mouse 18762 Prkcz protein kinase C, zeta NM_008860.3 88.8% 95.6% (many diffs)
30 mouse 18762 Prkcz protein kinase C, zeta XM_011250207.2 78% 84.6% (many diffs)
31 mouse 18762 Prkcz protein kinase C, zeta XM_006538655.3 72.7% 79.3% (many diffs)
32 mouse 18762 Prkcz protein kinase C, zeta NM_001039079.2 61% 67.7% (many diffs)
33 mouse 18762 Prkcz protein kinase C, zeta XM_006538656.2 61% 67.7% (many diffs)
34 mouse 18762 Prkcz protein kinase C, zeta XM_017320042.1 61% 67.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1842
ORF length:
1776
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc cagcaggacc ggccccaaga tggaagggag cggcggccgc gtccgcctca
121aggcgcatta cgggggggac atcttcatca ccagcgtgga cgccgccacg accttcgagg
181agctctgtga ggaagtgaga gacatgtgtc gtctgcacca gcagcacccg ctcaccctca
241agtgggtgga cagcgaaggt gacccttgca cggtgtcctc ccagatggag ctggaagagg
301ctttccgcct ggcccgtcag tgcagggatg aaggcctcat cattcatgtt ttcccgagca
361cccctgagca gcctggcctg ccatgtccgg gagaagacaa atctatctac cgccggggag
421ccagaagatg gaggaagctg taccgtgcca acggccacct cttccaagcc aagcgcttta
481acaggagagc gtactgcggt cagtgcagcg agaggatatg gggcctcgcg aggcaaggct
541acaggtgcat caactgcaaa ctgctggtcc ataagcgctg ccacggcctc gtcccgctga
601cctgcaggaa gcatatggat tctgtcatgc cttcccaaga gcctccagta gacgacaaga
661acgaggacgc cgaccttcct tccgaggaga cagatggaat tgcttacatt tcctcatccc
721ggaagcatga cagcattaaa gacgactcgg aggaccttaa gccagttatc gatgggatgg
781atggaatcaa aatctctcag gggcttgggc tgcaggactt tgacctaatc agagtcatcg
841ggcgcgggag ctacgccaag gttctcctgg tgcggttgaa gaagaatgac caaatttacg
901ccatgaaagt ggtgaagaaa gagctggtgc atgatgacga ggatattgac tgggtacaga
961cagagaagca cgtgtttgag caggcatcca gcaacccctt cctggtcgga ttacactcct
1021gcttccagac gacaagtcgg ttgttcctgg tcattgagta cgtcaacggc ggggacctga
1081tgttccacat gcagaggcag aggaagctcc ctgaggagca cgccaggttc tacgcggccg
1141agatctgcat cgccctcaac ttcctgcacg agagggggat catctacagg gacctgaagc
1201tggacaacgt cctcctggat gcggacgggc acatcaagct cacagacTAC GGCATGTGCA
1261AGGAAGGCCT GGGCCCTGGT GACACAACGA GCACTTTCTG CGGAACCCCG AATTACATCG
1321CCCCCGAAAT CCTGCGGGGA GAGGAGTACG GGTTCAGCGT GGACTGGTGG GCGCTGGGAG
1381TCCTCATGTT TGAGATGATG GCCGGGCGCT CCCCGTTCGA CATCATCACC GACAACCCGG
1441ACATGAACAC AGAGGACTAC CTTTTCCAAG TGATCCTGGA GAAGCCCATC CGGATCCCCC
1501GGTTCCTGTC CGTCAAAGCC TCCCATGTTT TAAAAGGATT TTTAAATAAG GACCCCAAAG
1561AGAGGCTCGG CTGCCGGCCA CAGACTGGAT TTTCTGACAT CAAGTCCCAC GCGTTCTTCC
1621GCAGCATAGA CTGGGACTTG CTGGAGAAGA AGCAGGCGCT CCCTCCATTC CAGCCACAGA
1681TCACAGACGA CTACGGTCTG GACAACTTTG ACACACAGTT CACCAGCGAG CCCGTGCAGC
1741TGACCCCAGA CGATGAGGAT GCCATAAAGA GGATCGACCA GTCAGAGTTC GAAGGCTTTG
1801AGTATATCAA CCCATTATTG CTGTCCACCG AGGAGTCGGT GTGCCCAACT TTCTTGTACa
1861aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1921ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)