Construct: ORF ccsbBroad304_14988

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018920.1_s304c1, BRDN0000459404
Derived from:
ccsbBroadEn_14988
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAP4K1 (11184)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14988
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11184 MAP4K1 mitogen-activated protein k... NM_001042600.3 99.2% 30.9% (many diffs)
2 human 11184 MAP4K1 mitogen-activated protein k... NM_007181.6 96.7% 30.4% (many diffs)
3 human 11184 MAP4K1 mitogen-activated protein k... XM_011526404.1 94.6% 29.5% (many diffs)
4 human 11184 MAP4K1 mitogen-activated protein k... XM_011526403.1 92.3% 29% (many diffs)
5 human 11184 MAP4K1 mitogen-activated protein k... XM_017026231.1 89.7% 25.5% (many diffs)
6 mouse 26411 Map4k1 mitogen-activated protein k... NM_008279.2 83.3% 28.8% (many diffs)
7 mouse 26411 Map4k1 mitogen-activated protein k... XM_006539995.2 82.7% 29.4% (many diffs)
8 mouse 26411 Map4k1 mitogen-activated protein k... XM_006539996.2 80.2% 29.9% (many diffs)
9 mouse 26411 Map4k1 mitogen-activated protein k... XM_006539997.3 80% 30% (many diffs)
10 mouse 26411 Map4k1 mitogen-activated protein k... XM_017322280.1 78.8% 23.9% (many diffs)
11 mouse 26411 Map4k1 mitogen-activated protein k... XM_006539998.2 76.9% 31.2% (many diffs)
12 mouse 26411 Map4k1 mitogen-activated protein k... XM_006539999.1 75.6% 24.8% (many diffs)
13 mouse 26411 Map4k1 mitogen-activated protein k... XM_006540000.2 73.6% 32.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
837
ORF length:
768
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggacgtcgtg gaccctgaca ttttcaatag agacccccgg gaccactatg
121acctgctaca gcggctgggt ggcggcacgt atggggaagt ctttaaggct cgagacaagg
181tgtcagggga cctggtggca ctgaagatgg tgaagatgga gcctgatgat gatgtctcca
241cccttcagaa ggaaatcctc atattgaaaa cttgccggca cgccaacatc gtggcctacc
301atgggagtta tctctggttg cagaaactct ggatctgcat ggaattctgt ggggctggtt
361ctctccagga catctaccaa gtgacaggct ccctgtcaga gctccagatt agctatgtct
421gccgggaagt gctccaggga ctggcctatt tgcactcaca gaagaagata cacagggaca
481tcaagggagc taacatcctc atcaatgatg ctggggaggt cagattggct gactttggca
541tctcggccca gattggggct acactggcca gacgcctctc tttcattggg acaccctact
601ggatggctcc ggaagtggca gctgtggccc tgaagggagg atacaatgag ctgtgtgaca
661tctggtccct gggcatcacg gccatcgaac tggccgagct acagccaccg ctctttgatg
721tgcaccctct cagagttctc ttcctcatga ccaagagtgg ctaccagcct ccccgactga
781aggaaaaagg caaatggtcg gctgccttcc acaacttcat caaagtcact tctgactaag
841agtcccaaga aacgacccag cgccaccaag atgctcagtc atcaactggt atcccagcct
901gggctgaatc gaggcctgat cctggatctt cttgacaaac tgaagaatcc cgggaaagga
961ccctccattg gggacattga ggatgaggag cccgagctac cccctgctat ccctcggcgg
1021atcagatcca cccaccgctc cagctctctg gggatcccag atgcagactg ctgtcggcgg
1081cacatggagt tcaggaagct ccgaggaatg gagaccagac ccccagccaa caccgctcgc
1141ctacagcctc ctcgagacct caggagcagc agccccagga agcaactgtc agagtcgtct
1201gacgatgact atgacgacgt ggacatcccc acccctgcag aggacacacc tcctccactt
1261ccccccaagc ccaagttccg ttctccatca gacgagggtc ctgggagcat gggggatgat
1321gggcagctga gcccgggggt gctggtccgg tgtgccagtg ggcccccacc aaacagcccc
1381cgtcctgggc ctcccccatc caccagcagc ccccacctca ccgcccattc agaaccctca
1441ctctggaacc caccctcccg ggagcttgac aagcccccac ttctgccccc caagaaggaa
1501aagatgaaga gaaagggatg tgcccttctc gtaaagttgt tcaatggctg ccccctccgg
1561atccacagca cggccgcctg gacacatccc tccaccaagg accagcacct gctcctgggg
1621gcagaggaag gcatcttcat cctgaaccgg aatgaccagg aggccacgct ggaaatgctc
1681tttcctagcc ggactacgtg ggtgtactcc atcaacaacg ttctcatgtc tctctcagga
1741aagacccccc ccttttattc tcatagcatc ccttgccctg ctggaacggg aaagagacca
1801gaagcaggaa acccccatcg gttcacatta gccccccacc gcctactgcn aanggagaaa
1861catggtttcc accaangatc caggacacca aagggctgcc gggcgtgctg tgtggcggag
1921ggtgcgagct ctgggggccc gttcctgtgc ggtgcattgg agacgtccgt tgtcctgctt
1981cagtggtacc agcccatgaa caaattcctg cttgtccggc aggtgctgtt cccactgccg
2041acgcctctgt ccgtgttcgc gctgctgacc gggccaggct ctgagctgcc cgctgtgtgc
2101atcggcgtga gccccgggcg gccggggaag tcggtgctct tccacacggt gcgctttggc
2161gcgctctctt gctggctggg cgagatgagc accgagcaca ggggacccgt gcaggtgacc
2221caggtagagg aagatatggt gatggtgttg atggatggct ctgtgaagct ggtgaccccg
2281gaggggtccc cagtccgggg acttcgcaca cctgagatcc ccatgaccga agcggtggag
2341gccgtggcta tggttggagg tcagcttcag gccttctgga agcatggagt gcaggtgtgg
2401gctctaggct cggatcagct gctacaggag ctgagagacc ctaccctcac tttccgtctg
2461cttggctccc ccaggcctgt agtggtggag acacgcccag tggatgatcc tactgctccc
2521agcaacctct acatccagga attgccaact ttcttgtaca aagtggttgg taagcctatc
2581cctaaccctc tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc
2641gttaagtcga caatcaacct ctggatta

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