Construct: ORF ccsbBroad304_16095
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005346.1_s304c1, BRDN0000411493
- Derived from:
- ccsbBroadEn_16095
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C9orf85 (138241)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_16095
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 138241 | C9orf85 | chromosome 9 open reading f... | NM_001365057.2 | 50.2% | 49.2% | 103_110delAAAATTAA;113_207delinsT |
| 2 | human | 138241 | C9orf85 | chromosome 9 open reading f... | NM_182505.5 | 22% | 21.6% | 103_110delAAAATTAA;113_471delinsT |
| 3 | human | 138241 | C9orf85 | chromosome 9 open reading f... | NM_001365053.2 | 19.3% | 18.9% | 103_110delAAAATTAA;113_537delinsT |
| 4 | human | 138241 | C9orf85 | chromosome 9 open reading f... | NR_157410.2 | 7.9% | 1_96del;198delG;203_1315del | |
| 5 | human | 138241 | C9orf85 | chromosome 9 open reading f... | NR_157408.2 | 2.7% | 1_96del;200C>G;202_3838del | |
| 6 | human | 138241 | C9orf85 | chromosome 9 open reading f... | NR_157409.2 | 2.6% | 1_96del;199_206delAAAATTAA;209_3925delinsT | |
| 7 | mouse | 66206 | 1110059E24Rik | RIKEN cDNA 1110059E24 gene | NM_025423.2 | 20.2% | 20.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 171
- ORF length:
- 105
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ctcccagaaa ggcaacgtgg ctcgttccag acctcagaag caccagaata 121 cgtttagctt caaaaatgac aagttcgata aaagtgtgca gaccaagtgt tnnccaactt 181 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 241 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta