Construct: ORF ccsbBroadEn_05013

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016389.1_s300c1, BRDN0000391229
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BROX (148362)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 148362 BROX BRO1 domain and CAAX motif ... NM_144695.4 100% 100%
2 human 148362 BROX BRO1 domain and CAAX motif ... XM_005273065.3 100% 100%
3 human 148362 BROX BRO1 domain and CAAX motif ... XM_011509214.3 100% 100%
4 human 148362 BROX BRO1 domain and CAAX motif ... XM_017000374.2 100% 100%
5 human 148362 BROX BRO1 domain and CAAX motif ... XM_006711173.4 96% 96% 1_51del
6 human 148362 BROX BRO1 domain and CAAX motif ... NM_001288579.1 92.2% 92.2% 305_306ins96
7 human 148362 BROX BRO1 domain and CAAX motif ... NM_001288580.1 92.2% 92.2% 305_306ins96
8 human 148362 BROX BRO1 domain and CAAX motif ... XM_011509213.3 90% 90.5% (many diffs)
9 human 148362 BROX BRO1 domain and CAAX motif ... XM_005273069.5 86.6% 86.6% 0_1ins165
10 human 148362 BROX BRO1 domain and CAAX motif ... XM_017000376.2 86.6% 86.6% 0_1ins165
11 human 148362 BROX BRO1 domain and CAAX motif ... XM_017000375.2 82.6% 83% (many diffs)
12 human 148362 BROX BRO1 domain and CAAX motif ... NM_001288581.1 82.2% 80.5% 988_989ins160;1014_1015ins59
13 human 148362 BROX BRO1 domain and CAAX motif ... XM_011509212.3 81.6% 81.6% 1_159del;464_465ins96
14 human 148362 BROX BRO1 domain and CAAX motif ... XR_001736986.1 32.4% 1_42del;711_712insGATCATACTTTATCCA;1260_3740del
15 mouse 71678 Brox BRO1 domain and CAAX motif ... NM_027861.2 85.8% 91.7% (many diffs)
16 mouse 71678 Brox BRO1 domain and CAAX motif ... XM_006535597.3 85.8% 91.7% (many diffs)
17 mouse 71678 Brox BRO1 domain and CAAX motif ... XM_006535598.3 85.8% 91.7% (many diffs)
18 mouse 71678 Brox BRO1 domain and CAAX motif ... XM_006535599.3 73.9% 79.5% (many diffs)
19 mouse 71678 Brox BRO1 domain and CAAX motif ... XM_006535600.3 61.6% 66.9% (many diffs)
20 mouse 71678 Brox BRO1 domain and CAAX motif ... XR_389720.3 30.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1302
ORF length:
1233
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GACCCATTGG TTTCATAGGA ACCCATTAAA AGCCACAGCT CCTGTGTCTT
121TTAATTACTA TGGTGTAGTC ACTGGCCCTT CTGCTTCAAA AATATGCAAT GACTTGAGGT
181CATCCAGGGC ACGACTCCTT GAACTGTTCA CTGATTTGAG CTGTAATCCA GAAATGATGA
241AGAATGCAGC AGATTCATAT TTCTCACTTT TACAAGGTTT CATAAATTCT TTGGATGAAT
301CTACCCAAGA AAGCAAGTTA CGATATATTC AAAATTTCAA GTGGACTGAT ACATTGCAAG
361GACAGGTTCC AAGTGCCCAG CAGGATGCTG TTTTTGAATT AATTTCCATG GGATTTAATG
421TAGCTTTATG GTATACCAAA TATGCTTCAA GACTGGCTGG AAAAGAAAAT ATAACAGAAG
481ATGAAGCAAA AGAAGTTCAT CGAAGCCTAA AGATTGCAGC TGGGATTTTT AAACATTTAA
541AGGAAAGTCA TCTCCCAAAA CTCATTACAC CTGCGGAAAA AGGAAGAGAT TTAGAGTCAC
601GACTCATAGA AGCATACGTT ATTCAATGTC AGGCTGAAGC TCAAGAAGTA ACAATTGCTC
661GAGCAATTGA ACTAAAACAT GCTCCTGGAC TAATTGCTGC ACTGGCGTAT GAAACAGCCA
721ATTTCTATCA AAAAGCTGAT CATACTTTAT CCAGTTTGGA GCCTGCATAT TCTGCCAAAT
781GGAGAAAATA TCTTCACTTG AAGATGTGTT TCTACACAGC TTATGCTTAC TGTTACCATG
841GTGAGACTTT ATTGGCTAGT GATAAATGCG GTGAAGCAAT CAGGTCTCTC CAAGAAGCAG
901AAAAATTGTA TGCAAAGGCA GAAGCACTGT GTAAAGAATA TGGAGAAACC AAAGGACCTG
961GACCAACAGT CAAACCTTCA GGACATCTGT TCTTTAGGAA ACTTGGAAAC CTTGTGAAGA
1021ACACCCTAGA AAAATGTCAG AGAGAAAATG GATTTATTTA CTTTCAAAAA ATTCCAACAG
1081AAGCCCCACA GCTGGAACTC AAAGCAAATT ATGGTCTCGT AGAGCCTATA CCTTTCGAAT
1141TTCCTCCTAC AAGTGTTCAG TGGACACCAG AAACATTGGC TGCATTTGAT CTCACCAAAA
1201GACCCAAGGA TGACAGTACT AAACCCAAAC CAGAAGAAGA AGTGAAACCT GTGAAAGAAC
1261CAGACATCAA ACCTCAAAAG GACACTGGGT GCTACATCTC CTTGCCAACT TTCTTGTACA
1321AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac

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