Construct: ORF ccsbBroadEn_07807
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015247.1_s300c1, BRDN0000456956
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF44 (22838)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22838 | RNF44 | ring finger protein 44 | NM_014901.5 | 99.9% | 100% | 894A>T |
| 2 | human | 22838 | RNF44 | ring finger protein 44 | XM_005265840.2 | 99.9% | 100% | 894A>T |
| 3 | human | 22838 | RNF44 | ring finger protein 44 | XM_005265841.4 | 99.9% | 100% | 894A>T |
| 4 | human | 22838 | RNF44 | ring finger protein 44 | XM_011534466.3 | 99.9% | 100% | 894A>T |
| 5 | human | 22838 | RNF44 | ring finger protein 44 | XM_006714831.2 | 99.2% | 99.3% | 464_465insGCTTATCCA;885A>T |
| 6 | human | 22838 | RNF44 | ring finger protein 44 | XM_006714832.2 | 89% | 89.1% | 464_465ins141;753A>T |
| 7 | human | 22838 | RNF44 | ring finger protein 44 | XM_005265842.5 | 81.1% | 81.2% | 0_1ins243;651A>T |
| 8 | human | 22838 | RNF44 | ring finger protein 44 | XM_005265843.2 | 81.1% | 81.2% | 0_1ins243;651A>T |
| 9 | human | 22838 | RNF44 | ring finger protein 44 | XM_005265844.3 | 81.1% | 81.2% | 0_1ins243;651A>T |
| 10 | human | 22838 | RNF44 | ring finger protein 44 | XM_005265845.4 | 81.1% | 81.2% | 0_1ins243;651A>T |
| 11 | human | 22838 | RNF44 | ring finger protein 44 | XM_011534467.2 | 81.1% | 81.2% | 0_1ins243;651A>T |
| 12 | human | 22838 | RNF44 | ring finger protein 44 | XM_024446002.1 | 70.2% | 70.3% | 0_1ins243;221_222ins141;510A>T |
| 13 | human | 22838 | RNF44 | ring finger protein 44 | XR_941091.2 | 33.3% | (many diffs) | |
| 14 | mouse | 105239 | Rnf44 | ring finger protein 44 | XM_006517020.1 | 89.1% | 91.4% | (many diffs) |
| 15 | mouse | 105239 | Rnf44 | ring finger protein 44 | NM_001146025.1 | 88.9% | 91.2% | (many diffs) |
| 16 | mouse | 105239 | Rnf44 | ring finger protein 44 | XM_006517018.3 | 88.9% | 91.2% | (many diffs) |
| 17 | mouse | 105239 | Rnf44 | ring finger protein 44 | XM_006517019.3 | 88.9% | 91.2% | (many diffs) |
| 18 | mouse | 105239 | Rnf44 | ring finger protein 44 | XM_011244446.1 | 88.9% | 91.2% | (many diffs) |
| 19 | mouse | 105239 | Rnf44 | ring finger protein 44 | XM_011244447.1 | 88.9% | 91.2% | (many diffs) |
| 20 | mouse | 105239 | Rnf44 | ring finger protein 44 | NM_001146026.1 | 83.9% | 87% | (many diffs) |
| 21 | mouse | 105239 | Rnf44 | ring finger protein 44 | NM_134064.2 | 73.3% | 77.7% | (many diffs) |
| 22 | mouse | 105239 | Rnf44 | ring finger protein 44 | XM_011244451.1 | 73.3% | 77.7% | (many diffs) |
| 23 | mouse | 105239 | Rnf44 | ring finger protein 44 | NM_001146027.1 | 73.2% | 77.5% | (many diffs) |
| 24 | mouse | 105239 | Rnf44 | ring finger protein 44 | XM_011244449.1 | 73.2% | 77.5% | (many diffs) |
| 25 | mouse | 105239 | Rnf44 | ring finger protein 44 | XM_011244450.1 | 73.2% | 77.5% | (many diffs) |
| 26 | mouse | 105239 | Rnf44 | ring finger protein 44 | XM_017315336.1 | 73.2% | 77.5% | (many diffs) |
| 27 | mouse | 105239 | Rnf44 | ring finger protein 44 | NR_027396.1 | 28% | (many diffs) | |
| 28 | mouse | 105239 | Rnf44 | ring finger protein 44 | NR_027395.1 | 28% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1365
- ORF length:
- 1296
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GCGACCATGG GCTCTGGCAG TGACTAGGTG GCCACCCTCC GCCCCCGTGG 121 GCCAGCGGCG ATTCTCTGCG GGACCTGGCA GCACCCCGGG CCAGCTCTGG GGAAGCCCTG 181 GCCTCGAGGG CCCCCTGGCC AGCCCGCCTG CCCGGGATGA GCGCTTACCC TCCCAGCAGC 241 CGCCGTCCCG ACCTCCACAC CTCCCCGTAG AGGAGCGCCG AGCCTCGGCT CCTGCCGGCG 301 GGAGCCCCCG AATGCTGCAC CCAGCCACCC AGCAGAGCCC GTTCATGGTT GATCTCCACG 361 AGCAGGTGCA CCAGGGACCT GTCCCTCTGT CCTACACGGT CACCACAGTG ACGACCCAAG 421 GCTTCCCCTT GCCTACAGGC CAGCACATCC CTGGCTGCAG TGCCCAGCAG CTCCCAGCAT 481 GCTCCGTGAT GTTCAGTGGG CAGCATTACC CCCTCTGCTG CCTCCCGCCC CCGCTTATCC 541 AGGCGTGCAC CATGCAGCAG CTGCCTGTGC CCTATCAGGC CTACCCCCAC CTCATCTCCA 601 GTGACCACTA CATCCTGCAC CCCCCACCAC CGGCCCCACC CCCCCAGCCC ACCCACATGG 661 CGCCCCTGGG GCAGTTTGTG TCTCTGCAGA CCCAGCACCC TCGGATGCCC CTCCAGCGGC 721 TCGACAACGA CGTGGACCTG CGTGGGGACC AGCCCTCCCT GGGCAGCTTC ACCTACTCCA 781 CCTCTGCGCC TGGCCCAGCC CTTTCCCCGT CGGTGCCCCT GCACTACCTG CCCCACGATC 841 CGCTGCACCA GGAGCTGTCC TTTGGTGTGC CATATTCTCA CATGATGCCA CGGAGACTGA 901 GCACCCAGAG ATACCGCCTG CAGCAGCCAC TGCCCCCGCC GCCCCCACCC CCACCCCCAC 961 CTCCCTACTA CCCCAGCTTC CTGCCCTACT TCCTCTCGAT GCTGCCAATG TCACCAACAG 1021 CAATGGGGCC CACCATCAGC CTGGACCTGG ACGTGGATGA TGTGGAGATG GAGAACTATG 1081 AGGCCCTCCT GAACCTGGCC GAGCGGCTGG GAGATGCCAA GCCCCGGGGT CTCACCAAAG 1141 CAGACATAGA GCAGCTCCCG TCGTACCGCT TTAACCCGGA CAGCCATCAG TCGGAGCAGA 1201 CGCTGTGTGT GGTCTGCTTC AGTGACTTCG AGGCGCGGCA GCTGCTCCGA GTCCTCCCCT 1261 GCAACCATGA GTTCCACACC AAGTGTGTTG ACAAGTGGTT GAAGGCCAAC CGGACGTGTC 1321 CCATCTGCCG GGCCGACGCC TCCGAGGTGC CCAGGGAGGC TGAGTTGCCA ACTTTCTTGT 1381 ACAAAGTTGG cattataaga aagcattgct tatcaatttg ttgcaacgaa c