Construct: ORF ccsbBroadEn_09375
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017802.1_s300c1, BRDN0000393813
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBA3E (112714)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112714 | TUBA3E | tubulin alpha 3e | NM_207312.3 | 99.7% | 99.7% | 849C>T;867C>T;1346C>A |
| 2 | human | 113457 | TUBA3D | tubulin alpha 3d | NM_080386.4 | 98.7% | 98.8% | (many diffs) |
| 3 | human | 7278 | TUBA3C | tubulin alpha 3c | NM_006001.3 | 96.2% | 98.8% | (many diffs) |
| 4 | human | 10376 | TUBA1B | tubulin alpha 1b | NM_006082.3 | 84.7% | 96% | (many diffs) |
| 5 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_001270399.1 | 84.1% | 96.4% | (many diffs) |
| 6 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_006009.4 | 84.1% | 96.4% | (many diffs) |
| 7 | human | 7277 | TUBA4A | tubulin alpha 4a | NM_006000.3 | 83.4% | 93.1% | (many diffs) |
| 8 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_032704.5 | 82.9% | 95.1% | (many diffs) |
| 9 | human | 7277 | TUBA4A | tubulin alpha 4a | NM_001278552.1 | 80.6% | 90% | (many diffs) |
| 10 | human | 51807 | TUBA8 | tubulin alpha 8 | NM_018943.3 | 80.1% | 89.5% | (many diffs) |
| 11 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_001270400.1 | 77.5% | 88.6% | (many diffs) |
| 12 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303115.2 | 76.2% | 87.3% | (many diffs) |
| 13 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303116.2 | 76.2% | 87.3% | (many diffs) |
| 14 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303117.2 | 76.2% | 87.3% | (many diffs) |
| 15 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303114.1 | 71.7% | 82.1% | (many diffs) |
| 16 | human | 51807 | TUBA8 | tubulin alpha 8 | NM_001193414.2 | 70.1% | 78.4% | (many diffs) |
| 17 | human | 7277 | TUBA4A | tubulin alpha 4a | XM_017004824.1 | 55.7% | 62.4% | (many diffs) |
| 18 | mouse | 22147 | Tuba3b | tubulin, alpha 3B | NM_009449.3 | 90.3% | 98.8% | (many diffs) |
| 19 | mouse | 22144 | Tuba3a | tubulin, alpha 3A | NM_009446.3 | 90% | 98.8% | (many diffs) |
| 20 | mouse | 22143 | Tuba1b | tubulin, alpha 1B | NM_011654.2 | 85.3% | 96% | (many diffs) |
| 21 | mouse | 22142 | Tuba1a | tubulin, alpha 1A | NM_011653.2 | 85% | 96.4% | (many diffs) |
| 22 | mouse | 22146 | Tuba1c | tubulin, alpha 1C | NM_009448.4 | 84.9% | 95.3% | (many diffs) |
| 23 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_009447.4 | 82.8% | 93.1% | (many diffs) |
| 24 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | XM_006496478.1 | 79.9% | 90% | (many diffs) |
| 25 | mouse | 53857 | Tuba8 | tubulin, alpha 8 | NM_017379.2 | 78.8% | 89.7% | (many diffs) |
| 26 | mouse | 22143 | Tuba1b | tubulin, alpha 1B | XM_006520756.1 | 77.9% | 87.4% | (many diffs) |
| 27 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_001313724.1 | 54.3% | 62.4% | (many diffs) |
| 28 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_001313723.1 | 45.1% | 52% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1416
- ORF length:
- 1350
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCG CGAGTGTATC TCTATCCACG TGGGGCAGGC GGGTGTCCAG ATCGGCAATG 121 CCTGCTGGGA ACTGTACTGC CTTGAACATG GAATTCAGCC CGATGGTCAA ATGCCAAGTG 181 ATAAAACCAT TGGTGGCGGG GACGACTCCT TCAACACGTT CTTCAGTGAG ACTGGAGCTG 241 GCAAGCACGT GCCCAGAGCA GTGTTTGTGG ACCTGGAGCC CACTGTGGTC GATGAAGTGC 301 GCACAGGGAC CTACAGGCAG CTCTTCCACC CAGAGCAGCT GATCACCGGG AAGGAAGATG 361 CAGCCAGTAA TTACGCCAGG GGCCATTACA CCATCGGCAA GGAGATTGTT GACCTAGTCC 421 TGGACCGGAT CCGCAAACTG GCGGATCTGT GCACAGGACT GCAGGGCTTC CTCATCTTCC 481 ACAGCTTTGG GGGCGGCACT GGCTCTGGGT TCGCATCTCT GCTCATGGAG CGGCTCTCAG 541 TGGATTACAG CAAGAAGTCC AAGCTAGAGT TTGCCATTTA CCCAGCCCCC CAGGTCTCCA 601 CAGCCGTGGT GGAGCCCTAC AACTCCATCC TAACCACCCA CACGACCCTG GAACATTCTG 661 ACTGTGCCTT CATGGTCGAC AATGAAGCCA TCTATGACAT ATGTCGGCGC AACCTGGACA 721 TTGAACGTCC CACGTACACC AACCTCAATC GCCTGATTGG GCAGATCGTG TCCTCCATCA 781 CGGCCTCCCT GCGATTTGAT GGGGCCCTGA ATGTGGACTT GACGGAATTC CAGACCAACC 841 TCGTGCCGTA CCCCCGCATC CACTTCCCCC TGGCCACCTA CGCCCCAGTC ATCTCAGCTG 901 AGAAGGCCTA CCATGAGCAG CTGTCTGTGG CTGAGATCAC CAATGCCTGC TTCGAGCCAG 961 CCAATCAGAT GGTCAAGTGT GACCCTCGCC ATGGCAAGTA CATGGCCTGC TGCATGTTGT 1021 ACAGGGGGGA CGTGGTCCCC AAAGACGTCA ATGCGGCCAT CGCCACCATC AAGACCAAGC 1081 GCACTATCCA GTTTGTGGAT TGGTGCCCGA CTGGATTTAA GGTGGGCATT AACTACCAGC 1141 CCCCCACAGT GGTCCCCGGG GGAGACCTGG CCAAGGTGCA GCGGGCCGTG TGCATGCTGA 1201 GCAACACCAC GGCCATTGCG GAGGCCTGGG CCCGCCTGGT CCATAAGTTC GATCTCATGT 1261 ATGCCAAGTG GGCCTTTGTG CACTGGTACG TGGGCGAAGG CATGGAAGAG GGAGAGTTCT 1321 CTGAGGCCCG CGAGGACCTG GCAGCTCTAG AGAAGGATTG TGAAGAGGTG GGCGTGGATT 1381 CCGTGGAAGC TGAGGCTGAA GAAGGCGAAG AATACTGCCC AACTTTCTTG TACAAAGTtg 1441 gcattataag aaagcattgc ttatcaattt gttgcaacga ac