Construct: ORF ccsbBroadEn_10033

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012955.1_s300c1, BRDN0000456729
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NEK5 (341676)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 341676 NEK5 NIMA related kinase 5 NM_199289.3 99.9% 99.8% 17T>C
2 human 341676 NEK5 NIMA related kinase 5 XM_017020557.1 90.7% 90.6% 1_216del;233T>C
3 human 341676 NEK5 NIMA related kinase 5 XM_017020555.1 87.5% 87.4% 1_216del;233T>C;1785_1786ins75
4 human 341676 NEK5 NIMA related kinase 5 XM_017020556.1 81.3% 79.7% (many diffs)
5 human 341676 NEK5 NIMA related kinase 5 XM_011535066.2 79.6% 79.2% (many diffs)
6 human 341676 NEK5 NIMA related kinase 5 NM_001365552.1 78.8% 77.1% (many diffs)
7 human 341676 NEK5 NIMA related kinase 5 XR_941575.2 72.8% (many diffs)
8 human 341676 NEK5 NIMA related kinase 5 XM_006719808.4 65% 61.8% (many diffs)
9 human 341676 NEK5 NIMA related kinase 5 XR_941574.2 63.3% (many diffs)
10 human 341676 NEK5 NIMA related kinase 5 XM_011535068.2 52.3% 51.7% (many diffs)
11 human 341676 NEK5 NIMA related kinase 5 XR_001749542.1 47.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2193
ORF length:
2124
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGATAAGTAC GATGCGATTA AGGCCATCGG GCAAGGTGCC TTCGGGAAAG
121CATACTTAGC TAAAGGGAAA TCAGATAGCA AGCACTGTGT CATAAAAGAG ATCAATTTTG
181AAAAGATGCC CATACAAGAA AAAGAAGCTT CAAAGAAAGA AGTGATTCTT CTGGAAAAGA
241TGAAACATCC CAACATTGTA GCCTTCTTCA ATTCATTTCA AGAGAATGGC AGGCTGTTTA
301TTGTAATGGA ATATTGTGAT GGAGGGGATC TCATGAAAAG GATCAATAGA CAACGGGGTG
361TGTTATTTAG TGAAGATCAG ATCCTCGGTT GGTTTGTACA GATTTCTCTA GGACTAAAAC
421ATATTCATGA CAGGAAGATA TTACACAGGG ACATAAAAGC TCAGAACATT TTTCTTAGCA
481AGAACGGAAT GGTGGCAAAG CTTGGGGACT TTGGTATAGC AAGAGTCCTG AATAATTCCA
541TGGAACTTGC TCGAACTTGT ATTGGAACAC CTTACTACCT GTCCCCAGAG ATCTGTCAGA
601ATAAACCCTA CAACAATAAA ACGGATATTT GGTCTCTTGG CTGTGTCTTA TATGAGCTCT
661GCACACTTAA ACATCCTTTT GAGGGTAACA ACTTACAGCA GCTGGTTCTG AAGATTTGTC
721AAGCACATTT TGCCCCAATA TCTCCGGGGT TTTCTCGTGA GCTCCATTCC TTGATATCTC
781AGCTCTTTCA AGTATCTCCT CGAGACCGAC CATCCATAAA TTCCATTTTG AAAAGGCCCT
841TTTTAGAGAA TCTTATTCCC AAATATTTGA CTCCTGAGGT CATTCAGGAA GAATTCAGTC
901ACATGCTTAT ATGCAGAGCA GGAGCGCCAG CTTCTCGACA TGCTGGGAAG GTGGTCCAGA
961AGTGTAAAAT ACAAAAAGTG AGATTCCAGG GAAAGTGCCC ACCAAGATCA AGGATATCTG
1021TGCCAATTAA AAGGAATGCT ATATTGCATA GAAATGAATG GAGACCACCA GCTGGAGCCC
1081AGAAGGCCAG ATCTATAAAA ATGATAGAAA GACCCAAAAT TGCTGCTGTC TGTGGACATT
1141ATGATTATTA TTATGCTCAA CTTGATATGC TGAGGAGGAG AGCCCACAAA CCAAGTTATC
1201ACCCTATTCC TCAAGAAAAT ACTGGAGTTG AGGATTACGG TCAGGAAACG AGGCATGGTC
1261CATCCCCAAG TCAATGGCCT GCTGAGTACC TTCAGAGAAA ATTTGAAGCT CAACAATATA
1321AGTTGAAAGT GGAGAAGCAA TTGGGTCTTC GTCCATCTTC TGCCGAGCCA AATTACAACC
1381AGAGACAAGA GCTAAGAAGT AATGGAGAAG AGCCTAGATT CCAGGAGCTG CCATTTAGGA
1441AAAACGAAAT GAAGGAACAG GAATATTGGA AGCAGTTAGA GGAAATACGC CAACAGTACC
1501ACAATGACAT GAAAGAAATT AGAAAGAAGA TGGGGAGAGA ACCAGAGGAG AACTCAAAAA
1561TAAGTCATAA AACCTATTTG GTGAAGAAGA GTAACCTGCC TGTCCATCAA GATGCATCTG
1621AGGGAGAAGC ACCTGTGCAG ATGGAATTTC GCTCTTGTTG CCCAGGCTGG AGTGCAATGG
1681CACGATCTTG GCTCACCGCA ACCTCCGCCT CCCAGGACAT TGAAAAAGAC TTGAAACAAA
1741TGAGGCTTCA GAACACAAAG GAAAGTAAAA ATCCAGAACA GAAATATAAA GCTAAGAAGG
1801GGGTAAAATT TGAAATTAAT TTAGACAAAT GTATTTCTGA TGAAAACATC CTCCAAGAGG
1861AAGAGGCAAT GGATATACCA AATGAAACTT TGACCTTTGA GGATGGCATG AAGTTTAAGG
1921AATATGAATG TGTAAAGGAG CATGGAGATT ATACAGACAA AGCATTTGAA AAACTTCACT
1981GCCCAGAAGC AGGGTTTTCC ACGCAGACTG TAGCTGCTGT GGGAAACAGG AGGCAGTGGG
2041ATGGAGGAGC GCCTCAGACT CTGCTGCAGA TGATGGCAGT GGCCGACATC ACCTCCACCT
2101GCCCCACGGG GCCTGACAGT GAGTCTGTGC TTAGCGTCAG TCGTCAGGAA GGGAAGACCA
2161AGGACCCGTA CAGCCCAGTG CTCATCCTGA TGTTGCCAAC TTTCTTGTAC AAAGTTGGca
2221ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.