Construct: ORF ccsbBroadEn_11198

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010523.1_s300c1, BRDN0000390786
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPM3 (7170)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7170 TPM3 tropomyosin 3 NM_001278191.2 87% 87.3% (many diffs)
2 human 7170 TPM3 tropomyosin 3 NM_001278188.2 70.1% 67.5% (many diffs)
3 human 7170 TPM3 tropomyosin 3 NM_001349679.2 63.7% 63.7% 1_270del
4 human 7170 TPM3 tropomyosin 3 NM_001278189.2 59.1% 57.6% (many diffs)
5 human 7170 TPM3 tropomyosin 3 NM_001364683.1 59.1% 57.6% (many diffs)
6 human 7170 TPM3 tropomyosin 3 NM_001043353.2 57.1% 56% (many diffs)
7 human 7170 TPM3 tropomyosin 3 NM_001043351.2 56.6% 55.6% (many diffs)
8 human 7170 TPM3 tropomyosin 3 NM_152263.4 55.4% 55.4% 1_381del
9 human 7170 TPM3 tropomyosin 3 NM_001043352.2 52.7% 50% (many diffs)
10 human 7170 TPM3 tropomyosin 3 NM_153649.4 52.3% 49.5% (many diffs)
11 human 7170 TPM3 tropomyosin 3 NM_001364682.1 51.5% 50.1% (many diffs)
12 human 7170 TPM3 tropomyosin 3 NM_001364681.2 49.8% 48.7% (many diffs)
13 human 7170 TPM3 tropomyosin 3 NM_001364679.2 49.5% 48.4% (many diffs)
14 human 7170 TPM3 tropomyosin 3 NM_001364680.2 45.7% 43.1% (many diffs)
15 human 7170 TPM3 tropomyosin 3 NM_001278190.1 44.7% 41.1% (many diffs)
16 human 147804 TPM3P9 tropomyosin 3 pseudogene 9 NR_003148.3 13.2% (many diffs)
17 human 7170 TPM3 tropomyosin 3 NR_103461.2 12.7% (many diffs)
18 mouse 59069 Tpm3 tropomyosin 3, gamma XM_006501841.3 66.6% 67.5% (many diffs)
19 mouse 59069 Tpm3 tropomyosin 3, gamma XM_017319678.1 60.8% 63.3% (many diffs)
20 mouse 59069 Tpm3 tropomyosin 3, gamma XM_006501838.1 60.7% 62.9% (many diffs)
21 mouse 59069 Tpm3 tropomyosin 3, gamma XM_006501836.2 59.4% 61.8% (many diffs)
22 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001271764.1 56.1% 57.2% (many diffs)
23 mouse 59069 Tpm3 tropomyosin 3, gamma XM_017319677.1 56.1% 57.2% (many diffs)
24 mouse 59069 Tpm3 tropomyosin 3, gamma XM_006501835.2 54.8% 55.9% (many diffs)
25 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001293749.1 54.4% 55.7% (many diffs)
26 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001253740.1 54.2% 55.6% (many diffs)
27 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001293748.1 52.9% 55% (many diffs)
28 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164254.1 51.6% 58% (many diffs)
29 mouse 59069 Tpm3 tropomyosin 3, gamma NM_001253738.1 49.7% 49.5% (many diffs)
30 mouse 22003 Tpm1 tropomyosin 1, alpha NM_001164248.1 45.1% 50.7% (many diffs)
31 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511042.3 45.1% 50.7% (many diffs)
32 mouse 22003 Tpm1 tropomyosin 1, alpha XM_006511041.1 45% 50.3% (many diffs)
33 mouse 59069 Tpm3 tropomyosin 3, gamma NM_022314.3 43.5% 43.1% (many diffs)
34 mouse 59069 Tpm3 tropomyosin 3, gamma XR_001783732.1 13.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
540
ORF length:
474
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA GGTTATTGAA AACCGGGCCT TAAAAGATGA AGAAAAGATG GAACTCCAGG
121AAATCCAACT CAAAGAAGCT AAGCACATTG CAGAAGAGGC AGATAGGAAG TATGAAGAGG
181TGGCTCGTAA GTTGGTGATC ATTGAAGGAG ACTTGGAACG CACAGAGGAA CGAGCTGAGC
241TGGCAGAGTC TAAGTGTTCT GAGCTGGAGG AGGAGCTGAA GAATGTCACC AACAACCTCA
301AGTCTCTTGA GGCTCAGGCG GAGAAGTACT CTCAAAAAGA AGATAAATAT GAGGAAGAAA
361TCAAGATTCT TACTGATAAA CTCAAGGAGG CAGAGACCCG TGCTGAGTTT GCTGAGAGAT
421CGGTAGCCAA GCTGGAAAAG ACAATTGATG ACCTGGAAGA TGAGCTCTAT GCCCAGAAAC
481TGAAGTACAA GGCCATTAGC GAGGAGCTGG ACCACGCCCT CAATGACATG ACCTCTATAT
541ACCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
601acgaac

Download FASTA (ORF) (Full)