Construct: ORF ccsbBroadEn_14715
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018828.1_s300c1, BRDN0000458124
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NME2 (4831)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018137.3 | 100% | 100% | |
| 2 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018138.1 | 100% | 100% | |
| 3 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018139.2 | 100% | 100% | |
| 4 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_002512.4 | 100% | 100% | |
| 5 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_000269.2 | 81.4% | 88.1% | (many diffs) |
| 6 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_198175.1 | 69.9% | 75.7% | (many diffs) |
| 7 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NM_001018136.3 | 56.9% | 56.9% | 1_345del |
| 8 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001198682.2 | 53.9% | 49.6% | 227_228ins113;246_247ins97 |
| 9 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NR_037149.2 | 35.8% | 1_673del;1130_1272del | |
| 10 | mouse | 18103 | Nme2 | NME/NM23 nucleoside diphosp... | NM_001077529.2 | 90.3% | 98% | (many diffs) |
| 11 | mouse | 18103 | Nme2 | NME/NM23 nucleoside diphosp... | NM_008705.5 | 90.3% | 98% | (many diffs) |
| 12 | mouse | 18102 | Nme1 | NME/NM23 nucleoside diphosp... | NM_008704.2 | 82.7% | 90.1% | (many diffs) |
| 13 | mouse | 18102 | Nme1 | NME/NM23 nucleoside diphosp... | XM_030245646.1 | 75.7% | 82.5% | (many diffs) |
| 14 | mouse | 433968 | Gm5566 | predicted pseudogene 5566 | XR_868740.1 | 69.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 245
- ORF end:
- 701
- ORF length:
- 456
- Sequence:
-
1 NNNNNANTTN NNNNNTNNNT NNNNNNNNCN NATTCAAACG AGAAATCTTA TCTTCTCGAA 61 TACTCAGTGA CACTCTTTTT TTTTCTTTTT TTCCTCCCAG TCCCCGCGTT GTAAAACGAC 121 GGCCAGTCTT AAGCTCGGGC CCCAAATAAT GATTTTATTT TGACTGATAG TGACCTGTTC 181 GTTGCAACAA ATTGATGAGC AATGCTTTTT TATAATGCCA ACTTTGTACA AAAAAGTTGG 241 CACCATGGCC AACCTGGAGC GCACCTTCAT CGCCATCAAG CCGGACGGCG TGCAGCGCGG 301 CCTGGTGGGC GAGATCATCA AGCGCTTCGA GCAGAAGGGA TTCCGCCTCG TGGCCATGAA 361 GTTCCTCCGG GCCTCTGAAG AACACCTGAA GCAGCACTAC ATTGACCTGA AAGACCGACC 421 ATTCTTCCCT GGGCTGGTGA AGTACATGAA CTCAGGGCCG GTTGTGGCCA TGGTCTGGGA 481 GGGGCTGAAC GTGGTGAAGA CAGGCCGAGT GATGCTTGGG GAGACCAATC CAGCAGATTC 541 AAAGCCAGGC ACCATTCGTG GGGACTTCTG CATTCAGGTT GGCAGGAACA TCATTCATGG 601 CAGTGATTCA GTAAAAAGTG CTGAAAAAGA AATCAGCCTA TGGTTTAAGC CTGAAGAACT 661 GGTTGACTAC AAGTCTTGTG CTCATGACTG GGTCTATGAA TTGCCAACTT TCTTGTACAA 721 AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG 781 TCAAAATAAA ATCATTATTN NCNNNCNNNN NNNTNNCNNC TATGTTGAGT CGTATTACAT 841 GGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AACAAAAAAA ATTTTAAAAT 901 ACTGGGGGTG AGTGGTTTGA GAGCCCGGGT TTGAACGAAT CCTAGGTACG AGACCATGTA 961 TAACTGCAGT GAAAGGTCTC TCTATCGCTG CGTACATCCT GCGTCGATGA AGCTAATAGT 1021 TCTTATCGTT CGATT