Construct: ORF ccsbBroadEn_14797
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018849.1_s300c1, BRDN0000458145
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKG1 (5592)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | NM_006258.4 | 99.8% | 63.4% | (many diffs) |
| 2 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | NM_001098512.3 | 91.6% | 52.5% | (many diffs) |
| 3 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_017016412.1 | 85.7% | 48.5% | (many diffs) |
| 4 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_017016413.1 | 85% | 48.2% | (many diffs) |
| 5 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_011539952.2 | 57.4% | 81.8% | (many diffs) |
| 6 | human | 5592 | PRKG1 | protein kinase cGMP-depende... | XM_024448078.1 | 41.1% | 4.8% | (many diffs) |
| 7 | mouse | 19091 | Prkg1 | protein kinase, cGMP-depend... | NM_011160.3 | 93% | 63.1% | (many diffs) |
| 8 | mouse | 19091 | Prkg1 | protein kinase, cGMP-depend... | NM_001013833.3 | 85.6% | 52.3% | (many diffs) |
| 9 | mouse | 19091 | Prkg1 | protein kinase, cGMP-depend... | XM_006526768.3 | 85.6% | 52.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 1454
- ORF length:
- 1356
- Sequence:
-
1 ATGGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GGCACCTTGC GGGATTTACA 121 GTACGCGCTC CAGGAGAAGA TCGAGGAGCT GAGGCAGCGG GATGCTCTCA TCGACGAGCT 181 GGAGCTGGAG TTGGATCAGA AGGACGAACT GATCCAGAAG CTGCAGAACG AGCTGGACAA 241 GTACCGCTCG GTGATCCGAC CAGCCACCCA GCAGGCGCAG AAGCAGAGCG CGAGCACCTT 301 GCAGGGCGAG CCGCGCACCA AGCGGCAGGC GATCTCCGCC GAGCCCACCG CCTTCGACAT 361 CCAGGATCTC AGCCATGTGA CCCTGCCCTT CTACCCCAAG AGCCCACAGT CCAAGGATCT 421 TATAAAGGAA GCTATCCTTG ACAATGACTT TATGAAGAAC TTGGAGCTGT CGCAGATCCA 481 GGAGATTGTG GATTGTATGT ACCCGGTGGA GTATGGCAAG GACAGTTGCA TCATCAAAGA 541 AGGAGACGTG GGGTCACTGG TGTATGTCAT GGAAGATGGT AAGGTTGAAG TTACAAAAGA 601 AGGTGTGAAG TTGTGTACCA TGGGTCCAGG AAAAGTGTTT GGGGAATTGG CTATTCTTTA 661 CAACTGTACC CGGACAGCGA CCGTCAAGAC TCTTGTAAAT GTAAAACTCT GGGCCATTGA 721 TCGACAATGT TTTCAAACAA TAATGATGAG GACAGGACTC ATCAAGCATA CCGAGTATAT 781 GGAATTTTTA AAAAGCGTTC CAATATTCCA GAGCCTTCCT GAAGAGATCC TCAGCAAGCt 841 tgctgatgtc cttgaagaga cccactatga aaatggagaa tatattatca ggcaaggtgc 901 aagaggggac accttcttta tcatcagcaa aggaacggta aatgtcactc gtgaagactc 961 accgagtgaa gacccagtct ttcttagaac tttaggaaaa ggagactggt ttggagagaa 1021 agccttgcag ggggaagatg tgagaacagc aaacgtaatt gctgcagaag ctgtaacctg 1081 ccttgtgatt gacagagact cttttaaaca tttgattgga gggctggatg atgtttctaa 1141 taaagcatat gaagatgcag aagctaaagc aaaatatgaa gctgaagcgg ctttcttcgc 1201 caacctgaag ctgtctgatt tcaacatcat tgataccctt ggagttggag gtttcggacg 1261 agtagaactg gtccagttga aaagtgaaga atccaaaacg tttgcaatga agattctcaa 1321 gaaacgtcac attgtggaca caagacagca ggagcacatc cgctcagaga agcagatcat 1381 gcagggggct cATTCCGATT TCATAAGTGA GACTGTACAA GAACATTTAA GGACAGCAAA 1441 TATTTGTATA TGTTGATGGA AGCTGTCTAG GTGGAGAGCT CTGGACCATT CTCAGGGATA 1501 GAGGTTCGTT TGAAGATTCT ACAACCAGAT TTTACACAGC ATGTGTGGTA GAAGCTTTTG 1561 CCTATCTGCA TTCCAAAGGA ATCATTTACA GGGACCTCAA GCCAGAAAAT CTCATCCTAG 1621 ATCACCGAGG TTATGCCAAA CTGGTTGATT TTGGCTTTGC AAAGAAAATA GGATTTGGAA 1681 AGAAAACATG GACTTTTTGT GGGACTCCAG AGTATGTAGC CCCAGAGATC ATCCTGAACA 1741 AAGGCCATGA CATTTCAGCC GACTACTGGT CACTGGGAAT CCTAATGTAT GAACTCCTGA 1801 CTGGCAGCCC ACCTTTCTCA GGCCCAGATC CTATGAAAAC CTATAACATC ATATTGAGGG 1861 GGATTGACAT GATAGAATTT CCAAAGAAGA TTGCCAAAAA TGCTGCTAAT TTAATTAAAA 1921 AACTATGCAG GGACAATCCA TCAGAAAGAT TAGGGAATTT GAAAAATGGA GTAAAAGACA 1981 TTCAAAAGCA CAAATGGTTT GAGGGCTTTA ACTGGGAAGG CTTAAGAAAA GGTACCTTGA 2041 CACCTCCTAT AATACCAAGT GTTGCATCAC CCACAGACAC AAGTAATTTT GACAGTTTCC 2101 CTGAGGACAA CGATGAACCA CCACCTGATG ACAACTCAGG ATGGGATATA GACTTCTTGC 2161 CAACTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT TGTTGCAACG 2221 AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTGCCAT CCAGCTGT