Construct: ORF TRCN0000474290

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010545.1_s317c1
Derived from:
ccsbBroadEn_14754
DNA Barcode:
CTTATGACAAAAGCCCCCCGACTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PHKB (5257)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474290
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5257 PHKB phosphorylase kinase regula... NM_001031835.3 99.9% 100% 297T>C;2154C>T
2 human 5257 PHKB phosphorylase kinase regula... NM_001363837.1 98.1% 98.2% (many diffs)
3 human 5257 PHKB phosphorylase kinase regula... NM_000293.3 96.8% 96.7% (many diffs)
4 mouse 102093 Phkb phosphorylase kinase beta NM_199446.2 88.3% 93% (many diffs)
5 mouse 102093 Phkb phosphorylase kinase beta XM_006530532.4 88.3% 93% (many diffs)
6 mouse 102093 Phkb phosphorylase kinase beta XM_006530533.4 88.3% 93% (many diffs)
7 mouse 102093 Phkb phosphorylase kinase beta XM_006530534.4 88.3% 93% (many diffs)
8 mouse 102093 Phkb phosphorylase kinase beta NM_001364412.1 87% 91.9% (many diffs)
9 mouse 102093 Phkb phosphorylase kinase beta XM_006530531.4 85.7% 90.5% (many diffs)
10 mouse 102093 Phkb phosphorylase kinase beta NM_001364411.1 83.9% 88.8% (many diffs)
11 mouse 102093 Phkb phosphorylase kinase beta XM_006530537.4 70% 73.5% (many diffs)
12 mouse 102093 Phkb phosphorylase kinase beta XR_878767.3 35.1% (many diffs)
13 mouse 102093 Phkb phosphorylase kinase beta XR_001778386.2 33.7% (many diffs)
14 mouse 102093 Phkb phosphorylase kinase beta XR_001778385.2 33.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3324
ORF length:
3258
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ctgctcacct gatgcagtcg tctctccgtc ttccgctttc ttaaggtctg
121gctcagttta tgaacctctt aaaagcatta atcttccaag acctgataat gaaactctct
181gggataagtt ggaccattat tacagaattg tcaagtcaac attgctgctg tatcaaagtc
241caactaccgg tctctttccc actaaaacat gcggtggtga ccagaaggcc aagatccagg
301acagcctata ctgcgctgct ggggcctggg ctttggctct tgcatacagg cgaattgatg
361acgacaaggg aaggacccat gagctggagc actcagctat aaaatgcatg agaggaattc
421tctactgcta tatgcgtcag gccgataagg tccagcagtt taagcaggat ccacgcccaa
481caacatgtct tcactctgtt ttcaatgtgc atacaggaga tgagttgctt tcctatgagg
541aatatggtca tcttcagata aatgcagtgt cactttatct cctttacctt gtggaaatga
601tttcctcagg actccagatt atctacaaca ctgatgaggt ctcttttatt caaaaccttg
661tattttgtgt ggaaagagtt taccgtgtgc ctgactttgg tgtctgggaa agaggaagca
721aatataataa tggcagcaca gagctacatt cgagctcggt tggtttagca aaagcagctc
781tagaagcaat taatggattc aacctttttg gcaaccaggg ctgttcgtgg tcagttatat
841ttgtggatct cgatgctcac aatcgcaaca ggcaaacttt gtgctcgctg ttacccagag
901aatcaagatc acataataca gatgctgccc tgctcccctg catcagttat cctgcatttg
961ccctggatga tgaagttctt tttagccaga cacttgataa agtggttaga aaattaaaag
1021gaaaatatgg atttaaacgt ttcttgagag atgggtatag aacatcattg gaagatccca
1081acagatgcta ctacaagcca gctgaaatta agctatttga tggcattgaa tgtgaatttc
1141ccatattttt cctttatatg atgattgatg gagtttttag aggcaatcct aagcaagtac
1201aggaatatca ggatcttttg actccagtac ttcatcatac cacagaagga tatcctgttg
1261taccaaagta ctattatgtg ccagctgact ttgtagaata tgaaaaaaat aaccctggta
1321gtcaaaaacg atttcctagc aactgtggcc gtgatggaaa actgtttctt tggggacaag
1381cactttatat catcgcaaaa ctcctggctg atgaacttat tagtcctaaa gacattgatc
1441ctgtccagcg ctatgtccca ctaaaggatc aacgtaacgt gagcatgagg ttttccaatc
1501agggcccact ggaaaatgac ttggtagttc atgtggcact tatagcagaa agccaaagac
1561ttcaagtttt tctgaacaca tatggtattc aaactcaaac tcctcaacaa gtagaaccca
1621ttcagatatg gcctcagcag gagcttgtga aagcttattt gcagctgggt atcaatgaaa
1681agttaggact ctctggaagg ccagacaggc ccattggctg cctcgggaca tcaaagattt
1741atcgcattct aggaaagact gtggtttgtt acccgattat tttcgaccta agtgatttct
1801acatgtctca ggatgttttc ctgctgatag atgacataaa gaatgcgctg cagttcatta
1861aacaatattg gaaaatgcat ggacgtccac ttttccttgt tctcatccgg gaagacaata
1921taagaggtag ccggttcaac cccatattag atatgctggc agcccttaaa aaaggaataa
1981ttggaggagt caaagttcat gtggatcgtc tacagacact aatatctgga gctgtggtag
2041aacaacttga tttcctacga atcagtgaca cagaagagct tccagaattt aagagttttg
2101aggaactaga acctcccaaa cattcaaaag tcaaacggca aagcagcacc cctagtgctc
2161ctgaactggg acagcagccg gatgtcaaca ttagtgaatg gaaggacaaa cccacccatg
2221aaattcttca aaaactgaat gattgcagtt gtctggctag ccaagccatc ctgctgggta
2281tactgctcaa aagagaaggc cccaacttca tcacaaagga aggtaccgtt tctgatcaca
2341ttgagagagt ctatagaaga gctggcagcc aaaaactttg gtcggttgta cgccgtgcag
2401caagtctttt aagtaaagta gtggacagcc tggccccatc cattactaat gttttagtgc
2461agggcaaaca ggtaactctg ggtgcctttg ggcatgaaga agaagttatc tctaatcctt
2521tgtctccaag agtgattcaa aacatcatct attataagtg taacacccat gatgagaggg
2581aagcggtcat tcagcaagaa ctggtcatcc atattggctg gatcatctcc aataaccctg
2641agttattcag tggcatgctg aaaatacgaa tcgggtggat catccatgcc atggagtatg
2701aacttcagat ccgtggcgga gacaagccag ccttggactt gtatcagctg tcacctagtg
2761aagttaaaca gcttctgctg gatattctgc agcctcaaca gaatggaaga tgttggctga
2821acaggcgtca gatcgatggg tctttgaata gaactcccac cgggttctat gaccgagtgt
2881ggCAGATTCT GGAGCGCACG CCCAATGGGA TCATTGTTGC TGGGAAGCAT TTGCCTCAGC
2941AACCAACCCT GTCAGATATG ACCATGTATG AGATGAATTT CTCTCTCCTT GTTGAAGACA
3001CGTTGGGAAA TATTGACCAG CCACAGTACA GACAGATCGT TGTAGAGTTA CTTATGGTTG
3061TATCCATTGT ACTGGAAAGA AACCCCGAGC TAGAATTTCA AGACAAAGTA GATCTAGACA
3121GACTGGTCAA AGAAGCATTT AATGAATTTC AAAAAGATCA GAGTCGGCTA AAGGAAATTG
3181AAAAACAAGA TGACATGACT TCCTTTTACA ACACTCCTCC CCTGGGAAAA AGAGGAACAT
3241GCAGCTATTT GACAAAGGCG GTGATGAATC TGCTGCTGGA AGGAGAAGTC AAGCCAAACA
3301ATGATGACCC GTGTCTGATT AGCTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
3361AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
3421TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC TTATGACAAA
3481AGCCCCCCGA CTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
3541att

Download FASTA (ORF) (Full)

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