Transcript: Mouse NM_001114087.2

Mus musculus PDZ and LIM domain 7 (Pdlim7), transcript variant b, mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Pdlim7 (67399)
Length:
1014
CDS:
92..760

Additional Resources:

NCBI RefSeq record:
NM_001114087.2
NBCI Gene record:
Pdlim7 (67399)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001114087.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000322470 GGAGACTGGGTACTGAATATT pLKO_005 230 CDS 100% 15.000 12.000 N Pdlim7 n/a
2 TRCN0000375191 GTTCATGCAAGACCCGGATGA pLKO_005 622 CDS 100% 4.050 3.240 N Pdlim7 n/a
3 TRCN0000178443 GCCTTTGAGCATGGAAATAAA pLKO.1 978 3UTR 100% 15.000 10.500 N Pdlim7 n/a
4 TRCN0000322543 TGCCTTTGAGCATGGAAATAA pLKO_005 977 3UTR 100% 15.000 10.500 N Pdlim7 n/a
5 TRCN0000322545 AGCTACAGAGCCCACGCTATA pLKO_005 681 CDS 100% 10.800 7.560 N Pdlim7 n/a
6 TRCN0000200458 GCTCTTGCCTACCACTTTCAT pLKO.1 836 3UTR 100% 5.625 3.938 N Pdlim7 n/a
7 TRCN0000375260 TCTTGGCCCTGTGGTACTACT pLKO_005 939 3UTR 100% 4.950 3.465 N Pdlim7 n/a
8 TRCN0000375190 CTGATGGAGGATACCGAAGAC pLKO_005 533 CDS 100% 4.050 2.835 N Pdlim7 n/a
9 TRCN0000181680 CTCTGCTTCCTTTCTCTGTTA pLKO.1 784 3UTR 100% 4.950 2.970 N Pdlim7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001114087.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02126 pDONR223 100% 88.6% 93.2% None (many diffs) n/a
2 ccsbBroad304_02126 pLX_304 0% 88.6% 93.2% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000466330 TCACCGTCTCGAGAAGACCACTGA pLX_317 54.5% 88.6% 93.2% V5 (not translated due to frame shift) (many diffs) n/a
4 ccsbBroadEn_11354 pDONR223 100% 75.4% 79.2% None (many diffs) n/a
5 ccsbBroad304_11354 pLX_304 0% 75.4% 79.2% V5 (many diffs) n/a
6 TRCN0000472937 ACAAGGGCGCAACGTACTCCCCAT pLX_317 57.8% 75.4% 79.2% V5 (many diffs) n/a
7 ccsbBroadEn_02125 pDONR223 100% 40.9% 39.8% None (many diffs) n/a
8 ccsbBroad304_02125 pLX_304 0% 40.9% 39.8% V5 (many diffs) n/a
9 TRCN0000474842 GCGCCAGGTAGACTTACACACAGT pLX_317 37.3% 40.9% 39.8% V5 (many diffs) n/a
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