Construct: ORF TRCN0000476937

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015946.1_s317c1
Derived from:
ccsbBroadEn_03845
DNA Barcode:
CTTTTGCCGTTTTACCGCAAAGCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MIER1 (57708)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476937
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57708 MIER1 MIER1 transcriptional regul... NM_001146113.1 100% 100%
2 human 57708 MIER1 MIER1 transcriptional regul... NM_001077704.3 85.4% 85.4% 1_189del
3 human 57708 MIER1 MIER1 transcriptional regul... NM_001077702.3 82.2% 82.2% 1_240del
4 human 57708 MIER1 MIER1 transcriptional regul... XM_017001932.1 81% 75.8% (many diffs)
5 human 57708 MIER1 MIER1 transcriptional regul... XM_017001933.1 81% 75.8% (many diffs)
6 human 57708 MIER1 MIER1 transcriptional regul... XM_017001934.1 81% 75.8% (many diffs)
7 human 57708 MIER1 MIER1 transcriptional regul... NM_001146111.1 78.7% 78.7% 1_240del;1280_1339del
8 human 57708 MIER1 MIER1 transcriptional regul... NM_001077703.3 76.1% 76.1% 1_348del
9 human 57708 MIER1 MIER1 transcriptional regul... NM_001146112.2 75% 70.3% (many diffs)
10 human 57708 MIER1 MIER1 transcriptional regul... XM_017001931.1 75% 70.3% (many diffs)
11 human 57708 MIER1 MIER1 transcriptional regul... XM_017001927.1 71.2% 66.8% (many diffs)
12 human 57708 MIER1 MIER1 transcriptional regul... XM_017001928.2 71.2% 66.8% (many diffs)
13 human 57708 MIER1 MIER1 transcriptional regul... XM_017001929.1 71.2% 66.8% (many diffs)
14 human 57708 MIER1 MIER1 transcriptional regul... XM_017001930.1 71.2% 66.8% (many diffs)
15 human 57708 MIER1 MIER1 transcriptional regul... NM_001077701.3 71.1% 66.7% (many diffs)
16 human 57708 MIER1 MIER1 transcriptional regul... NM_001146110.2 68.8% 64.6% (many diffs)
17 human 57708 MIER1 MIER1 transcriptional regul... NM_020948.4 68.8% 64.6% (many diffs)
18 human 57708 MIER1 MIER1 transcriptional regul... XM_005271076.3 68.8% 64.6% (many diffs)
19 human 57708 MIER1 MIER1 transcriptional regul... XM_017001924.1 68.8% 64.6% (many diffs)
20 human 57708 MIER1 MIER1 transcriptional regul... XM_017001925.1 68.8% 64.6% (many diffs)
21 human 57708 MIER1 MIER1 transcriptional regul... XM_017001926.1 68.8% 64.6% (many diffs)
22 human 57708 MIER1 MIER1 transcriptional regul... XM_017001923.1 67.3% 63.2% (many diffs)
23 human 57708 MIER1 MIER1 transcriptional regul... XM_017001921.1 66.8% 62.7% (many diffs)
24 human 57708 MIER1 MIER1 transcriptional regul... XM_017001922.1 66.8% 62.7% (many diffs)
25 human 57708 MIER1 MIER1 transcriptional regul... XM_017001919.1 66.4% 62.4% (many diffs)
26 human 57708 MIER1 MIER1 transcriptional regul... XM_017001920.1 66.4% 62.4% (many diffs)
27 human 57708 MIER1 MIER1 transcriptional regul... NM_001077700.3 64.4% 60.5% (many diffs)
28 human 57708 MIER1 MIER1 transcriptional regul... XM_011541865.2 61.8% 58.1% (many diffs)
29 human 57708 MIER1 MIER1 transcriptional regul... NM_001350530.2 60.5% 56.9% (many diffs)
30 human 57708 MIER1 MIER1 transcriptional regul... XR_001737345.1 20% (many diffs)
31 mouse 71148 Mier1 MEIR1 treanscription regulator XM_017320386.1 68.2% 67.7% (many diffs)
32 mouse 71148 Mier1 MEIR1 treanscription regulator NM_001286223.1 64.7% 64.3% (many diffs)
33 mouse 71148 Mier1 MEIR1 treanscription regulator NM_001039081.2 64.6% 64.2% (many diffs)
34 mouse 71148 Mier1 MEIR1 treanscription regulator NM_001286222.1 62.5% 62.2% (many diffs)
35 mouse 71148 Mier1 MEIR1 treanscription regulator NM_027696.3 62.5% 62.2% (many diffs)
36 mouse 71148 Mier1 MEIR1 treanscription regulator XM_006503401.3 62.5% 62.2% (many diffs)
37 mouse 71148 Mier1 MEIR1 treanscription regulator XM_006503402.1 62.5% 62.2% (many diffs)
38 mouse 71148 Mier1 MEIR1 treanscription regulator NM_001286221.1 59.4% 59.1% (many diffs)
39 mouse 71148 Mier1 MEIR1 treanscription regulator XM_006503399.2 58.8% 58.5% (many diffs)
40 mouse 71148 Mier1 MEIR1 treanscription regulator XM_006503398.2 57.2% 57% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1179
ORF length:
1110
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gccaattcat gaacttctca gcctttatgg ttatggtagt actgttcgac
121tacctgaaga agatgaggaa gaggaagaag aggaagaaga aggtgaagat gatgaagatg
181ctgataatga tgacaacagt ggctgtagtg gggaaaataa agaggagaat ataaaggatt
241catcaggtca ggaggatgaa actcagtctt ccaatgatga tccatcacaa tctgttgctt
301ctcaagatgc ccaggaaata atccgcccac gtcgatgtaa atattttgat acaaatagtg
361aagtagaaga agaatctgaa gaagatgaag attatattcc atcagaagac tggaaaaagg
421agattatggt gggctccatg tttcaagcag aaattccagt tggcatttgt agatacaaag
481aaaatgaaaa agtatatgaa aatgatgatc agctcctgtg ggaccctgag tacttaccag
541aagataaagt gattatattt cttaaagatg catctagaag aacaggtgat gagaagggtg
601tagaagcaat tcctgaagga tctcacataa aagacaatga acaggcttta tatgaattgg
661ttaaatgcaa ttttgataca gaagaagcat tgagaagatt aagatttaat gtaaaagcag
721ctagagagga attaTCTGTT TGGACAGAGG AAGAGTGTAG AAATTTTGAA CAAGGGCTGA
781AGGCCTATGG AAAGGATTTT CATTTGATTC AGGCTAATAA AGTCCGAACA AGGTCAGTTG
841GTGAATGTGT AGCATTCTAT TACATGTGGA AAAAATCTGA ACGTTATGAT TTCTTTGCTC
901AGCAAACACG ATTTGGAAAG AAGAAATATA ATCTTCATCC TGGTGTAACG GATTACATGG
961ATCGTCTTCT AGACGAAAGT GAAAGTGCTG CATCTAGTCG AGCACCATCC CCTCCCCCAA
1021CTGCATCAAA CAGTAGTAAC AGCCAGTCTG AGAAAGAAGA TGGCACTGTA AGCACTGCTA
1081ATCAAAATGG AGTGTCATCT AATGGACCAG GCATACTCCA AATGCTTCTT CCAGTTCATT
1141TTTCAGCCAT CAGTTCAAGA GCCAATGCCT TTTTAAAATT GCCAACTTTC TTGTACAAAG
1201TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1261GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1321ACGACTTTTG CCGTTTTACC GCAAAGCAAC GCGTTAAGTC gacaatcaac ctctggatta
1381caaaatttgt gaaagatt

Download FASTA (ORF) (Full)