Transcript: Human NM_001257173.2

Homo sapiens DEAD-box helicase 19B (DDX19B), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-09-26
Taxon:
Homo sapiens (human)
Gene:
DDX19B (11269)
Length:
3322
CDS:
512..1624

Additional Resources:

NCBI RefSeq record:
NM_001257173.2
NBCI Gene record:
DDX19B (11269)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001257173.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000365061 GTGCTGTTGTCAAGACCAATG pLKO_005 291 5UTR 100% 6.000 8.400 N DDX19B n/a
2 TRCN0000369971 CATGGGTTTCAATCGTCCATC pLKO_005 511 5UTR 100% 4.050 5.670 N DDX19B n/a
3 TRCN0000051128 GCCATGCTTAGCCAAGTAGAA pLKO.1 635 CDS 100% 4.950 3.960 N DDX19B n/a
4 TRCN0000377419 ACGCATTGCCACTGATGCTTG pLKO_005 546 CDS 100% 4.050 3.240 N DDX19B n/a
5 TRCN0000051129 CCTGAACTGAAGCTAGCTTAT pLKO.1 752 CDS 100% 10.800 7.560 N DDX19B n/a
6 TRCN0000365062 CTGAACTGAAGCTAGCTTATG pLKO_005 753 CDS 100% 10.800 7.560 N DDX19B n/a
7 TRCN0000051132 GCAGAGAAGACAGATGAAGAA pLKO.1 317 5UTR 100% 4.950 3.465 N DDX19B n/a
8 TRCN0000377369 TATGAGCTCGCCCTCCAAACA pLKO_005 698 CDS 100% 4.950 3.465 N DDX19B n/a
9 TRCN0000051130 CACAGAACTTAATTGCCCAAT pLKO.1 576 CDS 100% 4.050 2.835 N DDX19B n/a
10 TRCN0000365063 AGCCAAGTAGAACCTGCAAAC pLKO_005 644 CDS 100% 6.000 3.600 N DDX19B n/a
11 TRCN0000365060 CACAGGAGACAAGTGCGTTCA pLKO_005 1668 3UTR 100% 4.050 2.430 N DDX19B n/a
12 TRCN0000050220 CGCGGCATTGATGTTGAACAA pLKO.1 1367 CDS 100% 4.950 2.475 Y DDX19A n/a
13 TRCN0000151004 GATTTGGACGAGATTGAGAAA pLKO.1 1592 CDS 100% 4.950 2.475 Y DDX19A n/a
14 TRCN0000155545 CCAGAAGATCAGTGAGCAGAT pLKO.1 802 CDS 100% 4.050 2.025 Y DDX19A n/a
15 TRCN0000155474 GCACAGCATGAACATCCTGAA pLKO.1 1522 CDS 100% 4.050 2.025 Y DDX19A n/a
16 TRCN0000152824 GAATCCTGACAATGAGACCTA pLKO.1 1432 CDS 100% 2.640 1.320 Y DDX19A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001257173.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02664 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02664 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000492319 TCACCATTCCTGCGTCCACGACGC pLX_317 2.8% 100% 100% V5 n/a
4 ccsbBroadEn_02663 pDONR223 100% 77.2% 77.2% None 0_1ins327 n/a
5 ccsbBroad304_02663 pLX_304 0% 77.2% 77.2% V5 0_1ins327 n/a
6 TRCN0000472192 ATGCACTATTTATGCCGATATGCA pLX_317 32.3% 77.2% 77.2% V5 0_1ins327 n/a
7 ccsbBroadEn_03576 pDONR223 100% 74.5% 76.3% None (many diffs) n/a
8 ccsbBroad304_03576 pLX_304 0% 74.5% 76.3% V5 (many diffs) n/a
9 TRCN0000472365 CATCTACGCACTTAACAACACTCT pLX_317 29.8% 74.5% 76.3% V5 (many diffs) n/a
Download CSV

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