Construct: ORF TRCN0000492319

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011440.1_s317c1
Derived from:
ccsbBroadEn_02664
DNA Barcode:
TCACCATTCCTGCGTCCACGACGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX19B (11269)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492319
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11269 DDX19B DEAD-box helicase 19B NM_001014449.3 100% 100%
2 human 11269 DDX19B DEAD-box helicase 19B NM_001257173.2 100% 100%
3 human 11269 DDX19B DEAD-box helicase 19B NM_001257174.2 100% 100%
4 human 55308 DDX19A DEAD-box helicase 19A NM_001320525.1 91.8% 94% (many diffs)
5 human 11269 DDX19B DEAD-box helicase 19B NM_001257175.2 88.6% 88.6% 0_1ins126
6 human 55308 DDX19A DEAD-box helicase 19A NM_001320526.1 86.3% 87.5% (many diffs)
7 human 11269 DDX19B DEAD-box helicase 19B NM_001014451.3 81.6% 78.2% (many diffs)
8 human 11269 DDX19B DEAD-box helicase 19B NM_001257172.2 80.7% 77.4% (many diffs)
9 human 55308 DDX19A DEAD-box helicase 19A NM_001320522.1 77.3% 77.5% (many diffs)
10 human 11269 DDX19B DEAD-box helicase 19B NM_007242.7 77.2% 77.2% 1_327del
11 human 11269 DDX19B DEAD-box helicase 19B NM_001363938.1 76.4% 76.4% 1_342del
12 human 55308 DDX19A DEAD-box helicase 19A NM_001320527.1 75.3% 73.8% (many diffs)
13 human 55308 DDX19A DEAD-box helicase 19A NM_018332.5 74.5% 76.3% (many diffs)
14 human 55308 DDX19A DEAD-box helicase 19A NR_135296.1 34.5% (many diffs)
15 human 55308 DDX19A DEAD-box helicase 19A XR_002957830.1 27.1% (many diffs)
16 mouse 13680 Ddx19a DEAD (Asp-Glu-Ala-Asp) box ... XM_006530650.3 85.3% 92.7% (many diffs)
17 mouse 234733 Ddx19b DEAD (Asp-Glu-Ala-Asp) box ... NM_172284.5 69.9% 75.5% (many diffs)
18 mouse 13680 Ddx19a DEAD (Asp-Glu-Ala-Asp) box ... NM_007916.2 69.3% 75.3% (many diffs)
19 mouse 234733 Ddx19b DEAD (Asp-Glu-Ala-Asp) box ... NM_001190786.1 67.8% 73.2% (many diffs)
20 mouse 234733 Ddx19b DEAD (Asp-Glu-Ala-Asp) box ... NM_001190800.1 67.5% 71.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1176
ORF length:
1110
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg tttcaatcgt ccatccaaga tacaagagaa cgcattgcca ctgatgcttg
121ctgagccccc acagaactta attgcccaat ctcagtctgg tactggtaaa acagctgcct
181tcgtgctggc catgcttagc caagtagaac ctgcaaacaa atacccccag tgtctatgtc
241tctccccaac gtatgagctc gccctccaaa caggaaaagt gattgaacaa atgggcaaat
301tttaccctga actgaagcta gcttatgctg ttcgaggcaa taaattggaa agaggccaga
361agatcagtga gcagattgtc attggcaccc ctgggactgt gctggactgg tgctccaagc
421tcaagttcat tgatcccaag aaaatcaagg tgtttgttct ggatgaggct gatgtcatga
481tagccactca gggccaccaa gatcagagca tccgcatcca gaggatgctg cccaggaact
541gccagatgct gcttttctcc gccacctttg aagactctgt gtggaagttt gcccagaaag
601tggtcccaga cccaaacgtt atcaaactga agcgtgagga agagaccctg gacaccatca
661agcagtacta tgtcctgtgc agcagcagag acgagaagtt ccaggccttg tgtaacctct
721acggggccat caccattgct caagccatga tcttctgcca tactcgcaaa acagctagtt
781ggctggcagc agagctctca aaagaaggcc accaggtggc tctgctgagt ggggagatga
841tggtggaaca gagggctgca gtgattgagc gcttccgaga gggcaaagag aaggttttgg
901tgaccaccaa cgtgtgtgcc cgcggcattg atgttgaaca agtgtctgtc gtcatcaact
961ttgatcttcc cgtggacaag gacgggaatc ctgacaatga gacctacctg caccggatcg
1021ggcgcacggg ccgctttggc aagaggggcc tggcagtgaa catggtggac agcaagcaca
1081gcatgaacat cctgaacaga atccaggagc attttaataa gaagatagaa agattggaca
1141cagatgattt ggacGAGATT GAGAAAATAG CCAACTGCCC AACTTTCTTG TACAAAGTGG
1201TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1261CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1321ATCACCATTC CTGCGTCCAC GACGCACGCG TTAAGTCgac aatcaacctc tggattacaa
1381aatttgtgaa agatt

Download FASTA (ORF) (Full)