Construct: ORF TRCN0000472365

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000533.1_s317c1
Derived from:
ccsbBroadEn_03576
DNA Barcode:
CATCTACGCACTTAACAACACTCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX19A (55308)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472365
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55308 DDX19A DEAD-box helicase 19A NM_018332.5 100% 100%
2 human 11269 DDX19B DEAD-box helicase 19B NM_007242.7 95% 96% (many diffs)
3 human 55308 DDX19A DEAD-box helicase 19A NM_001320522.1 93.5% 93.5% 293_294ins93
4 human 11269 DDX19B DEAD-box helicase 19B NM_001363938.1 92.9% 91.8% (many diffs)
5 human 11269 DDX19B DEAD-box helicase 19B NM_001014451.3 89% 89.7% (many diffs)
6 human 11269 DDX19B DEAD-box helicase 19B NM_001257172.2 87% 85.6% (many diffs)
7 human 55308 DDX19A DEAD-box helicase 19A NM_001320525.1 80.1% 79.9% (many diffs)
8 human 11269 DDX19B DEAD-box helicase 19B NM_001014449.3 74.5% 76.3% (many diffs)
9 human 11269 DDX19B DEAD-box helicase 19B NM_001257173.2 74.5% 76.3% (many diffs)
10 human 11269 DDX19B DEAD-box helicase 19B NM_001257174.2 74.5% 76.3% (many diffs)
11 human 55308 DDX19A DEAD-box helicase 19A NM_001320526.1 68.6% 68.6% 0_1ins450
12 human 11269 DDX19B DEAD-box helicase 19B NM_001257175.2 66.8% 67.7% (many diffs)
13 human 55308 DDX19A DEAD-box helicase 19A NM_001320527.1 59.1% 57.4% (many diffs)
14 human 55308 DDX19A DEAD-box helicase 19A NR_135296.1 40.3% 1_163del;549_550ins218;1380_2796del
15 human 55308 DDX19A DEAD-box helicase 19A XR_002957830.1 36.4% 1_121del;1304_2289del;2542_3939del
16 mouse 13680 Ddx19a DEAD (Asp-Glu-Ala-Asp) box ... NM_007916.2 90.9% 97.4% (many diffs)
17 mouse 234733 Ddx19b DEAD (Asp-Glu-Ala-Asp) box ... NM_172284.5 88.3% 93.3% (many diffs)
18 mouse 234733 Ddx19b DEAD (Asp-Glu-Ala-Asp) box ... NM_001190800.1 85.8% 89.1% (many diffs)
19 mouse 234733 Ddx19b DEAD (Asp-Glu-Ala-Asp) box ... NM_001190786.1 85.1% 87.4% (many diffs)
20 mouse 13680 Ddx19a DEAD (Asp-Glu-Ala-Asp) box ... XM_006530650.3 72.9% 78.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1500
ORF length:
1434
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61tctgcatggc caccgactcg tgggccctgg cggtggacga gcaggaagcg gctgtcaagt
121cgatgaccaa tttgcagatc aaggaagaga aagtcaaagc agataccaat ggtattatca
181aaaccagtac cactgccgag aaaacagatg aagaggagaa agaggacaga gctgcccagt
241ccttactcaa caagctgatc agaagcaacc ttgttgataa cacaaaccaa gtggaagtcc
301tgcaacggga tccaaactcc cctctgtact cggtgaagtc gtttgaagag cttcggctga
361aaccacagct tctccaggga gtctatgcca tgggcttcaa tcgaccctcc aagatacaag
421agaacgcatt acccatgatg cttgctgaac ccccacagaa tctgattgcc cagtctcagt
481ctggcactgg taaaacagct gcctttgtct tagccatgct cagccgagtg gagccatcag
541acagataccc ccagtgtctg tgcctctccc caacatatga gctggcgctt caaacaggaa
601aagtgattga gcagatgggc aaattttacc cagaactgaa gcttgcctat gccgttcgag
661gcaataaatt ggaaagaggc cagaagatca gtgagcagat tgtcattggc acccctggga
721ccgtgctgga ctggtgctcc aagctcaagt tcattgatcc caagaaaatc aaggtgtttg
781ttctggatga ggctgatgtc atgatagcca ctcagggcca ccaagatcag agcatccgca
841tccagaggat gctgcccagg aactgccaga tgctgctttt ctccgccacc tttgaagact
901ctgtgtggaa gtttgcccag aaagtggtcc cagacccaaa tgttatcaaa ctgaagcgtg
961aggaagagac cctggatacc atcaagcagt actatgtcct gtgcagcagc agagacgaga
1021agttccaggc cttgtgtaac ctctacgggg ccatcaccat tgctcaagcc atgatcTTCT
1081GCCATACTCG CAAAACAGCT AGTTGGCTGG CAGCAGAGCT CTCAAAAGAA GGCCACCAGG
1141TGGCTCTGCT GAGTGGGGAG ATGATGGTGG AGCAGAGGGC TGCGGTGATT GAGCGCTTCC
1201GAGAGGGCAA AGAGAAGGTT TTGGTGACCA CCAACGTGTG TGCCCGCGGC ATTGATGTTG
1261AACAAGTGTC TGTCGTCATC AACTTTGATC TTCCCGTGGA CAAGGACGGG AATCCTGACA
1321ATGAGACCTA CCTGCACCGG ATCGGGCGCA CGGGCCGCTT TGGCAAGAGG GGCCTGGCAG
1381TGAACATGGT GGACAGCAAG CACAGCATGA ACATCCTGAA CAGAATCCAG GAGCATTTTA
1441ATAAGAAGAT AGAAAGATTG GACACAGATG ATTTGGACGA GATTGAGAAA ATAGCCAACT
1501GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1561GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1621TTTATATATC TTGTGGAAAG GACGACATCT ACGCACTTAA CAACACTCTA CGCGTTAAGT
1681Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)