Construct: ORF ccsbBroad304_02663
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014020.1_s304c1, BRDN0000399134
- Derived from:
- ccsbBroadEn_02663
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX19B (11269)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02663
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11269 | DDX19B | DEAD-box helicase 19B | NM_007242.7 | 100% | 100% | |
| 2 | human | 11269 | DDX19B | DEAD-box helicase 19B | NM_001363938.1 | 96.4% | 95.2% | (many diffs) |
| 3 | human | 55308 | DDX19A | DEAD-box helicase 19A | NM_018332.5 | 95% | 96% | (many diffs) |
| 4 | human | 11269 | DDX19B | DEAD-box helicase 19B | NM_001014451.3 | 93.5% | 93.5% | 296_297ins93 |
| 5 | human | 11269 | DDX19B | DEAD-box helicase 19B | NM_001257172.2 | 90.1% | 88.9% | (many diffs) |
| 6 | human | 55308 | DDX19A | DEAD-box helicase 19A | NM_001320522.1 | 89% | 89.7% | (many diffs) |
| 7 | human | 11269 | DDX19B | DEAD-box helicase 19B | NM_001014449.3 | 77.2% | 77.2% | 0_1ins327 |
| 8 | human | 11269 | DDX19B | DEAD-box helicase 19B | NM_001257173.2 | 77.2% | 77.2% | 0_1ins327 |
| 9 | human | 11269 | DDX19B | DEAD-box helicase 19B | NM_001257174.2 | 77.2% | 77.2% | 0_1ins327 |
| 10 | human | 55308 | DDX19A | DEAD-box helicase 19A | NM_001320525.1 | 76.8% | 78.7% | (many diffs) |
| 11 | human | 11269 | DDX19B | DEAD-box helicase 19B | NM_001257175.2 | 68.4% | 68.4% | 0_1ins453 |
| 12 | human | 55308 | DDX19A | DEAD-box helicase 19A | NM_001320526.1 | 66.7% | 67.6% | (many diffs) |
| 13 | human | 55308 | DDX19A | DEAD-box helicase 19A | NM_001320527.1 | 58.5% | 57.3% | (many diffs) |
| 14 | human | 55308 | DDX19A | DEAD-box helicase 19A | NR_135296.1 | 39% | (many diffs) | |
| 15 | human | 55308 | DDX19A | DEAD-box helicase 19A | XR_002957830.1 | 34.6% | (many diffs) | |
| 16 | mouse | 234733 | Ddx19b | DEAD (Asp-Glu-Ala-Asp) box ... | NM_172284.5 | 90.2% | 97.2% | (many diffs) |
| 17 | mouse | 13680 | Ddx19a | DEAD (Asp-Glu-Ala-Asp) box ... | NM_007916.2 | 88.8% | 94.7% | (many diffs) |
| 18 | mouse | 234733 | Ddx19b | DEAD (Asp-Glu-Ala-Asp) box ... | NM_001190800.1 | 87.8% | 93.1% | (many diffs) |
| 19 | mouse | 234733 | Ddx19b | DEAD (Asp-Glu-Ala-Asp) box ... | NM_001190786.1 | 86.3% | 90.8% | (many diffs) |
| 20 | mouse | 13680 | Ddx19a | DEAD (Asp-Glu-Ala-Asp) box ... | XM_006530650.3 | 72.2% | 77.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1503
- ORF length:
- 1437
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cactgactca tgggccctgg cggtggacga gcaggaagct gcggctgagt 121 cgttgagcaa cttgcatctt aaggaagaga aaatcaaacc agataccaat ggtgctgttg 181 tcaagaccaa tgccaatgca gagaagacag atgaagaaga gaaagaggac agagctgccc 241 agtccttact caacaagctg atcagaagca accttgttga taacacaaac caagtggaag 301 tcctgcagcg ggatccaaac tcccctctgt actcggtgaa gtcttttgaa gagcttcggc 361 tgaaaccaca gcttctccaa ggagtctatg ccatgggttt caatcgtcca tccaagatac 421 aagagaacgc attgccactg atgcttgctg agcccccaca gaacttaatt gcccaatctc 481 agtctggtac tggtaaaaca gctgccttcg tgctggccat gcttagccaa gtagaacctg 541 caaacaaata cccccagtgt ctatgtctct ccccaacgta tgagctcgcc ctccaaacag 601 gaaaagtgat tgaacaaatg ggcaaatttt accctgaact gaagctagct tatgctgttc 661 gaggcaataa attggaaaga ggccagaaga tcagtgagca gattgtcatt ggcacccctg 721 ggactgtgct ggactggtgc tccaagctca agttcattga tcccaagaaa atcaaggtgt 781 ttgttctgga tgaggctgat gtcatgatag ccactcaggg ccaccaagat cagagcatcc 841 gcatccagag gatgctgccc aggaactgcc agatgctgct tttctccgcc acctttgaag 901 actctgtgtg gaagtttgcc cagaaagtgg tcccagaccc aaacgttatc aaactgaagc 961 gtgaggaaga gaccctggac accatcaagc agtactatgt cctgtgcagc agcagagacg 1021 agaagttcca ggccttgtgt aacctctacg gggccatcac cattgctcaa gccatgatct 1081 tctgccatac tcgcaaaaca gctagttggc tggcagcaga gctctcaaaa gaaggccacc 1141 aggtggctct gctgagtggg gagatgatgg tggaacagag ggctgcagtg attgagcgct 1201 tccgagaggg caaagagaag gttttggtga ccaccaacgt gtgtgcccgc ggcattgatg 1261 ttgaacaagt gtctgtcgtc atcaactttg atcttcccgt ggacaaggac gggaatcctg 1321 acaatgagac ctacctgcac cggatcgggc gcacgggccg ctttggcaag aggggcctgg 1381 cagtgaacat ggtggacagc aagcacagca tgaacatcct gaacagaatc caggagcatt 1441 ttaataagaa gatagaaaga ttggacacag atgatttgga cgagattgag aaaatagcca 1501 actgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1561 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1621 tctggatta