Construct: ORF ccsbBroad304_02664

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011440.1_s304c1, BRDN0000404283
Derived from:
ccsbBroadEn_02664
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX19B (11269)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02664
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11269 DDX19B DEAD-box helicase 19B NM_001014449.3 100% 100%
2 human 11269 DDX19B DEAD-box helicase 19B NM_001257173.2 100% 100%
3 human 11269 DDX19B DEAD-box helicase 19B NM_001257174.2 100% 100%
4 human 55308 DDX19A DEAD-box helicase 19A NM_001320525.1 91.8% 94% (many diffs)
5 human 11269 DDX19B DEAD-box helicase 19B NM_001257175.2 88.6% 88.6% 0_1ins126
6 human 55308 DDX19A DEAD-box helicase 19A NM_001320526.1 86.3% 87.5% (many diffs)
7 human 11269 DDX19B DEAD-box helicase 19B NM_001014451.3 81.6% 78.2% (many diffs)
8 human 11269 DDX19B DEAD-box helicase 19B NM_001257172.2 80.7% 77.4% (many diffs)
9 human 55308 DDX19A DEAD-box helicase 19A NM_001320522.1 77.3% 77.5% (many diffs)
10 human 11269 DDX19B DEAD-box helicase 19B NM_007242.7 77.2% 77.2% 1_327del
11 human 11269 DDX19B DEAD-box helicase 19B NM_001363938.1 76.4% 76.4% 1_342del
12 human 55308 DDX19A DEAD-box helicase 19A NM_001320527.1 75.3% 73.8% (many diffs)
13 human 55308 DDX19A DEAD-box helicase 19A NM_018332.5 74.5% 76.3% (many diffs)
14 human 55308 DDX19A DEAD-box helicase 19A NR_135296.1 34.5% (many diffs)
15 human 55308 DDX19A DEAD-box helicase 19A XR_002957830.1 27.1% (many diffs)
16 mouse 13680 Ddx19a DEAD (Asp-Glu-Ala-Asp) box ... XM_006530650.3 85.3% 92.7% (many diffs)
17 mouse 234733 Ddx19b DEAD (Asp-Glu-Ala-Asp) box ... NM_172284.5 69.9% 75.5% (many diffs)
18 mouse 13680 Ddx19a DEAD (Asp-Glu-Ala-Asp) box ... NM_007916.2 69.3% 75.3% (many diffs)
19 mouse 234733 Ddx19b DEAD (Asp-Glu-Ala-Asp) box ... NM_001190786.1 67.8% 73.2% (many diffs)
20 mouse 234733 Ddx19b DEAD (Asp-Glu-Ala-Asp) box ... NM_001190800.1 67.5% 71.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1176
ORF length:
1110
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg tttcaatcgt ccatccaaga tacaagagaa cgcattgcca ctgatgcttg
121ctgagccccc acagaactta attgcccaat ctcagtctgg tactggtaaa acagctgcct
181tcgtgctggc catgcttagc caagtagaac ctgcaaacaa atacccccag tgtctatgtc
241tctccccaac gtatgagctc gccctccaaa caggaaaagt gattgaacaa atgggcaaat
301tttaccctga actgaagcta gcttatgctg ttcgaggcaa taaattggaa agaggccaga
361agatcagtga gcagattgtc attggcaccc ctgggactgt gctggactgg tgctccaagc
421tcaagttcat tgatcccaag aaaatcaagg tgtttgttct ggatgaggct gatgtcatga
481tagccactca gggccaccaa gatcagagca tccgcatcca gaggatgctg cccaggaact
541gccagatgct gcttttctcc gccacctttg aagactctgt gtggaagttt gcccagaaag
601tggtcccaga cccaaacgtt atcaaactga agcgtgagga agagaccctg gacaccatca
661agcagtacta tgtcctgtgc agcagcagag acgagaagtt ccaggccttg tgtaacctct
721acggggccat caccattgct caagccatga tcttctgcca tactcgcaaa acagctagtt
781ggctggcagc agagctctca aaagaaggcc accaggtggc tctgctgagt ggggagatga
841tggtggaaca gagggctgca gtgattgagc gcttccgaga gggcaaagag aaggttttgg
901tgaccaccaa cgtgtgtgcc cgcggcattg atgttgaaca agtgtctgtc gtcatcaact
961ttgatcttcc cgtggacaag gacgggaatc ctgacaatga gacctacctg caccggatcg
1021ggcgcacggg ccgctttggc aagaggggcc tggcagtgaa catggtggac agcaagcaca
1081gcatgaacat cctgaacaga atccaggagc attttaataa gaagatagaa agattggaca
1141cagatgattt ggacgagatt gagaaaatag ccaactgccc aactttcttg tacaaagtgg
1201ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1261ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.